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Human Gremlin-2(GREM2) ELISA kit

  • 中文名称：
    人Gremlin-2(GREM2)酶联免疫试剂盒
  • 货号：
    CSB-EL009893HU
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human GREM2 ELISA Kit was designed for the quantitative measurement of Human GREM2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 6.25 pg/mL-2500 pg/mL and the sensitivity is 6.25 pg/mL.

  • 别名：
    BMP antagonist 2 ELISA Kit; CKTSF1B2 ELISA Kit; Cysteine knot superfamily 1 ELISA Kit; Cysteine knot superfamily 1 BMP antagonist 2 ELISA Kit; DAN domain family member 3 ELISA Kit; DAND 3 ELISA Kit; DAND3 ELISA Kit; GREM 2 ELISA Kit; Grem2 ELISA Kit; GREM2_HUMAN ELISA Kit; Gremlin 2 ELISA Kit; Gremlin 2 cysteine knot superfamily homolog ELISA Kit; Gremlin-2 ELISA Kit; Gremlin2 ELISA Kit; PRDC ELISA Kit; Protein related to DAN and cerberus ELISA Kit
  • 缩写：
    GREM2
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    6.25 pg/mL-2500 pg/mL
  • 灵敏度：
    6.25 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Signal Transduction
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human GREM2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 88-100  
    1:2 Average % 95  
    Range % 84-101  
    1:4 Average % 93  
    Range % 87-100  
    1:8 Average % 90  
    Range % 87-101  
  • 回收率：
    The recovery of human GREM2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 88 85-93  
    EDTA plasma (n=4) 95 90-99  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average    
    2500 0.155 0.149 0.152    
    469 0.342 0.352 0.347    
    78 0.559 0.569 0.564    
    22 1.001 0.963 0.982    
    6.25 1.543 1.510 1.527    
    0 2.184 2.086 2.135    
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Cytokine that inhibits the activity of BMP2 and BMP4 in a dose-dependent manner, and thereby modulates signaling by BMP family members. Contributes to the regulation of embryonic morphogenesis via BMP family members. Antagonizes BMP4-induced suppression of progesterone production in granulosa cells.
  • 基因功能参考文献：
    1. TT genotype of the rs4454537 polymorphism protective against the development of osteoporosis PMID: 30014930
    2. Our study confirmed that GREM2 is a candidate gene for tooth agenesis, which mutations can explain, however, only a small fraction of the genetic contribution to the pathogenesis of this anomaly. PMID: 28992378
    3. Data show that the GREMLIN 2 (GREM2) expression during Induced Pluripotent Stem Cell (hiPS) cell cardiac differentiation follows the expression pattern of cardiac-specific genes. PMID: 28125926
    4. The structure of Grem2-GDF5 complex has revealed a number of key findings for DAN-family mediated BMP2 inhibition. PMID: 27524626
    5. This study showed that si-Grem2 increased the BMP-2-induced osteogenic differentiation of hBMSCs via the BMP-2/Smad/Runx2 pathway. PMID: 27335248
    6. The present study shows that the Grem2 heparin/HS and BMP2-binding epitopes are unique and independent, where the Grem2-BMP2 complex exhibits a significant increase in binding affinity toward heparin moieties that appear to be partially independent of the Grem2 heparin/HS-binding epitope. PMID: 28104757
    7. Gremlin 2 inhibits adipocyte differentiation through activation of Wnt/beta-catenin signaling PMID: 26239165
    8. mutations associated with tooth malformations PMID: 26416033
    9. GREM2 variants have been identified in atrial fibrillation cohort studies, demonstrating abnormalities in cardiac excitation - supra ventricular tachycardia and atrial fibrillation. PMID: 25443231
    10. The minor allele of rs4454537 is significantly associated with low bone density at the total hip of southern Chinese people. Our study further suggests GREM2 as a novel susceptibility gene for osteoporosis. PMID: 23902946
    11. Through regulation of bone morphogenetic protein signaling, GREM2 is required for cardiac laterality and atrial differentiation during embryonic development. PMID: 23223679
    12. and that a genetic variant in the FMN2/GREM2 locus influences GREM2 expression in osteoblasts and thereby trabecular number and thickness as well as fracture risk. PMID: 23437003

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  • 相关疾病：
    Tooth agenesis, selective, 9 (STHAG9)
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    DAN family
  • 数据库链接：

    HGNC: 17655

    OMIM: 608832

    KEGG: hsa:64388

    STRING: 9606.ENSP00000318650

    UniGene: Hs.98206