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  4. Horse Hepcidin(HAMP) ELISA kit

Horse Hepcidin(HAMP) ELISA kit

  • 中文名称：
    马铁调素(HAMP)酶联免疫试剂盒
  • 货号：
    CSB-EL010124HO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Horse HAMP ELISA Kit was designed for the quantitative measurement of Horse HAMP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL.

  • 别名：
    HEPC, HEPCIDIN, HFE2B, LEAP-1, LEAP1, PLTR, liver-expressed antimicrobial peptide|putative liver tumor regressor
  • 缩写：
    HAMP
  • 种属：
    Equus caballus (Horse)
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    7.8 ng/mL-500 ng/mL
  • 灵敏度：
    1.95 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%        
    Three samples of known concentration were tested twenty times on one plate to assess.    
    Inter-assay Precision (Precision between assays): CV%<10%        
    Three samples of known concentration were tested in twenty assays to assess.      
                   
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of horse HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
    　 Sample Serum(n=4)    
    1:1 Average % 91    
    Range % 86-95    
    1:2 Average % 102    
    Range % 97-107    
    1:4 Average % 91    
    Range % 85-97    
    1:8 Average % 97    
    Range % 91-103    
  • 回收率：
    The recovery of horse HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
     
    Sample Type Average % Recovery Range    
    Serum (n=5) 95 89-98    
    EDTA plasma (n=4) 97 90-100    
                   
                   
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
     
    ng/ml OD1 OD2 Average Corrected    
    500 2.556 2.530 2.543 2.441    
    250 2.065 2.100 2.083 1.981    
    125 1.621 1.569 1.595 1.493    
    62.5 1.078 1.098 1.088 0.986    
    31.2 0.676 0.698 0.687 0.585    
    15.6 0.377 0.398 0.388 0.286    
    7.8 0.230 0.244 0.237 0.135    
    0 0.101 0.102 0.102 　    
  • 数据处理：
  • 货期：
    3-5 working days

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