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Dog Hepcidin(HAMP) ELISA kit

  • 中文名称：
    犬铁调素(HAMP)酶联免疫试剂盒
  • 货号：
    CSB-EL010124DO
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Dog HAMP ELISA Kit was designed for the quantitative measurement of Dog HAMP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL.

  • 别名：
    HAMP ELISA Kit; Hepcidin ELISA Kit
  • 缩写：
    HAMP
  • Uniprot No.：
  • 种属：
    Canis lupus familiaris (Dog) (Canis familiaris)
  • 样本类型：
    serum, plasma
  • 检测范围：
    3.12 ng/mL-200 ng/mL
  • 灵敏度：
    0.78 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of dog HAMP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 107
    Range % 96-111
    1:2 Average % 100
    Range % 90-104
    1:4 Average % 97
    Range % 90-101
    1:8 Average % 91
    Range % 82-95
  • 回收率：
    The recovery of dog HAMP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 91 84-95
    EDTA plasma (n=4) 107 99-111
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected
    200 1.971 1.912 1.942 1.810
    100 1.482 1.477 1.480 1.348
    50 0.976 0.988 0.982 0.850
    25 0.546 0.556 0.551 0.419
    12.5 0.402 0.378 0.390 0.258
    6.25 0.263 0.259 0.261 0.129
    3.12 0.178 0.172 0.175 0.043
    0 0.131 0.133 0.132 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

问答及客户评论

■ 常见问题解答
Q:

My name is Thitapa, student of veterinary medicine, Chulalongkorn university from Thailand.
Recently we are working on a study about canine hepcidin and I would like to know how much it is for 1 dog hepcidin ELISA test kit 96-well (I couldn't find it on the website ,only found the 24-T one) Moreover, if you have any evidence about any studies that your product had been used to evaluate hepcidin level please attach the information. We would be really appreciated.

A:
Thanks for your concern on Cusabio and your email!We can supply both size of Dog Hepcidin(HAMP) ELISA kit ,24T & 96T.
#CSB-EL010124DO,Dog Hepcidin(HAMP) ELISA kit -96T
Regarding this kit,we tested the normal dog serum ,plasma samples with 0 dilutions and we got the results falling in 12.5--100ng/ml.You can take this for your reference.As long as the content of the sample falls in the detection range of this kit,it can be detected out.And I will send you manual reference by email later.
If you are interested to order it,pls kindly contact and enquire local distributor.

靶点详情

  • 功能：
    Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of ferroportin/SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes.; Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
  • 基因功能参考文献：
    1. The objectives of this study were to clone and sequence the canine hepcidin gene and to gather preliminary data about tissue expression of hepcidin in dogs. PMID: 15570559
  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Hepcidin family
  • 数据库链接：