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Ms4a1

MS4A1基因编码CD20蛋白,该抗原为一种疏水性4次跨膜蛋白,分子量大约为35kDa。CD20也叫B淋巴细胞表面抗原B1、Bp35、白细胞表面抗原Leu-16等等。CD20只在B淋巴细胞上表达,参与调节B细胞活化和增殖,作为钙离子通道发挥作用。除在正常B细胞中表达外,CD20还在B细胞来源的淋巴瘤、白血病等的肿瘤细胞表达,以及涉及免疫疾病和炎症疾病的B细胞中表达,所以CD20抗原成为淋巴癌、白血病和某些自体免疫等疾病治疗的目标靶点。目前,针对这些疾病的CD20治疗主要有三种方式,包括抗CD20单克隆抗体、CAR-T和双特异性抗体。自1997年全球首款抗CD20单抗——罗氏的利妥昔单抗获批以来,全球已经批准多款抗CD20单抗。相关数据统计显示,目前有高达90多种针对CD20的生物制剂处于临床开发阶段。

CUSABIO-哺乳包膜VLPs平台成功开发MS4A1全长膜蛋白(4次跨膜),展示了完整生物活性。

热销产品

Recombinant Human B-lymphocyte antigen CD20(MS4A1)-VLPs (Active) CSB-MP015007HU

验证数据

High Specificity Validated by Western blot (WB)

CSB-MP015007HU is detected by Mouse anti-6*His monoclonal antibody.

Excellent Bioactivity Validated by Functional ELISA

Measured by its binding ability in a functional ELISA. Immobilized human CD20 at 2 μg/ml can bind Anti-CD20 recombinant antibody (CSB-RA015007A1HU), the EC50 is 3.243-7.085 ng/mL.

Correct Structure Validated
by TEM

The presence of VLP-like structures was confirmed by TEM.

MS4A1 Recombinant Monoclonal Antibody CSB-RA015007MA4HU

验证数据

WB
CSB-RA015007MA4HU WB

IHC image of CSB-RA015007MA4HU diluted at 1:200 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB

IHC
CSB-RA015007MA4HU IF

Immunofluorescence staining of Hela cell with CSB-RA015007MA4HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

IF
CSB-RA015007MA4HU IF

Immunofluorescence staining of MCF7 cell with CSB-RA015007MA4HU at 1:150, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L)

Ms4a1 Antibodies

Ms4a1 for Mus musculus (Mouse)

Ms4a1 for Homo sapiens (Human)

Ms4a1 for H, M, R

Ms4a1 Proteins

Ms4a1 Proteins for Canis familiaris (Dog) (Canis lupus familiaris)

Ms4a1 Proteins for Mus musculus (Mouse)

Ms4a1 Proteins for Homo sapiens (Human)

Ms4a1 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)

Ms4a1 ELISA Kit

Ms4a1 ELISA Kit for Homo sapiens (Human)