IL23

The Binding Activity of IL12B&IL12A with Anti-IL12/IL23 monoclonal antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human IL12B&IL12A (CSB-MP4155HU) at 1 μg/mL can bind Anti-IL12/IL23 recombinant antibody, the EC₅₀ is 1.042-1.545 ng/mL.

IHC image of CSB-RA011587MA1HU diluted at 1:50 and staining in paraffin-embedded human lymphnode tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-human polymer IgG labeled by HRP and visualized using 0.05% DAB.