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  4. Human Interleukin-23 subunit alpha(IL-23A) Elisa kit

Human Interleukin-23 subunit alpha(IL-23A) Elisa kit

  • 中文名称：
    人白介素23A(IL-23A)酶联免疫试剂盒
  • 货号：
    CSB-E17339h
  • 规格：
    96T/48T
  • 价格：
    ￥3600/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Human IL23A ELISA Kit was designed for the quantitative measurement of Human IL23A protein in serum, plasma, cell culture supernates, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.

  • 别名：
    IL 23 A ELISA Kit; IL 23 ELISA Kit; IL 23 subunit alpha ELISA Kit; IL 23A ELISA Kit; IL 23p19 ELISA Kit; IL-23 subunit alpha ELISA Kit; IL-23-A ELISA Kit; IL-23p19 ELISA Kit; IL12B ELISA Kit; IL23 ELISA Kit; Il23a ELISA Kit; IL23A_HUMAN ELISA Kit; IL23P19 ELISA Kit; interleukin 12B ELISA Kit; Interleukin 23 alpha subunit p19 ELISA Kit; Interleukin 23 p19 subunit ELISA Kit; interleukin 23 subunit alpha ELISA Kit; interleukin 23 subunit p19 ELISA Kit; interleukin six; G CSF related factor ELISA Kit; Interleukin-23 subunit alpha ELISA Kit; Interleukin-23 subunit p19 ELISA Kit; JKA3 induced upon T cell activation ELISA Kit; MGC79388 ELISA Kit; P19 ELISA Kit; SGRF ELISA Kit
  • 缩写：
  • Uniprot No.：
  • 种属：
    Homo sapiens (Human)
  • 样本类型：
    serum, plasma, cell culture supernates, tissue homogenates, cell lysates
  • 检测范围：
    62.5 pg/mL-4000 pg/mL
  • 灵敏度：
    15.6 pg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Sandwich
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%
    Three samples of known concentration were tested twenty times on one plate to assess.
    Inter-assay Precision (Precision between assays): CV%<10%
    Three samples of known concentration were tested in twenty assays to assess.
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of human IL-23A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)
    1:1 Average % 88
    Range % 83-97
    1:2 Average % 97
    Range % 91-104
    1:4 Average % 84
    Range % 81-88
    1:8 Average % 89
    Range % 81-96
  • 回收率：
    The recovery of human IL-23A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range
    Serum (n=5) 101 94-101
    EDTA plasma (n=4) 107 100-114
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    pg/ml OD1 OD2 Average Corrected
    4000 1.762 1.845 1.804 1.628
    2000 1.001 1.022 1.012 0.836
    1000 0.669 0.667 0.668 0.492
    500 0.407 0.416 0.412 0.236
    250 0.297 0.277 0.287 0.111
    125 0.253 0.246 0.250 0.074
    62.5 0.220 0.216 0.218 0.042
    0 0.172 0.179 0.176 　
  • 数据处理：
  • 货期：
    3-5 working days

产品评价

靶点详情

  • 功能：
    Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
  • 基因功能参考文献：
    1. IL23 and IL17 have roles in the pathogenesis of Tunisian pemphigus foliaceus PMID: 30116153
    2. Effect of polymorphisms in IL-12B p40, IL-17A and IL-23 A/G genes on the response of psoriatic patients to narrowband UVB. PMID: 29763989
    3. Epigenetic control of IL-23 expression in keratinocytes is important for chronic skin inflammation. PMID: 29650963
    4. IL-23 binding to its receptor promotes the migration and invasion of gastric cancer cells by inducing epithelial-to-mesenchymal transition through the STAT3 signaling pathway. PMID: 29574157
    5. TNF-alpha plays an important role for the autocrine stimulation of IL-23 production by 6-sulfo LacNAc(+) dendritic cells. PMID: 27315572
    6. The expression of IL-17 and IL-12 in patients with lupus miliaris disseminatus faciei is reported in patients and healthy controls. PMID: 27515793
    7. SIGIRR is both a negative regulator of TLR4 and a positive regulator of TLR7/8. PMID: 28869081
    8. interleukin 23 (IL-23)/T-helper 17 (TH 17) immune axis has been identified as a major immune pathway in psoriasis disease pathogenesis PMID: 28653490
    9. Serum IL-17 and IL-23 values positively correlated with serum total IgE levels. Serum levels of IL-10 were lower in children with atopy and dyslipidemia than patients with dyslipidemia. PMID: 28692864
    10. IL-23/IL-17 axis and biochemical markers in the pathogenesis of Type 2 Diabetes PMID: 28757426
    11. Higher levels of TGF-beta mRNA were observed in biopsies taken from healthy controls, and the IL-23 mRNA levels were significantly increased in the peri-implantitis group (P < 0.0001). No differences in IL-17 mRNA levels were observed between the two groups (P > 0.05). PMID: 27062688
    12. clinical significance of IL-17 and IL-23 in the pathogenesis of different types of gastric neoplasms in humans, is reported. PMID: 27869179
    13. this study shows neuroprotective effects of G-CSF administration in microglia-mediated reactive T cell activation in vitro in a mouse model PMID: 28646409
    14. NOD2 up-regulates TLR2-mediated IL-23p19 expression via increasing c-Rel activation in Paneth cell-like cells PMID: 27563808
    15. Serum IL-23 and IL-17 levels were elevated in patients with aneurysmal subarachnoid hemorrhage (aSAH) showing upregulation of IL-23/IL-17 inflammatory axis after aSAH. Serum IL-23 and IL-17 showed differential correlations with post hemorrhagic complications and no correlation with clinical outcome. PMID: 28609751
    16. IL-23, alone and in combination with IL-18 and sRAGE, identified bacterial meningitis with excellent accuracy. Following validation, these markers could aid clinicians in diagnosis of bacterial meningitis and decision-making regarding prolongation of antibiotic therapy PMID: 29394248
    17. Serum IL-17F predicted increased knee bone marrow lesion scores in females only among patients with knee osteoarthritis. PMID: 27836676
    18. Our discoveries demonstrate that autocrine IL-23 contribute to ovarian cancer malignancy through promoting the self-renewal of CD133+ ovarian cancer stem-like cells, and this suggests that IL-23 and its signaling pathway might serve as therapeutic targets for the treatment of ovarian cancer. PMID: 27738346
    19. Study of IL-23 showed reduced levels of expression in aged humans compared to young. PMID: 28509479
    20. IL-23 activates gammadelta T cells in early stage PNS, which amplifies the Th17 cell population and leads to kidney injury through the secretion of IL-21. PMID: 29119640
    21. serum IL-23 levels reflect obstructive sleep apnea syndrome -related systemic inflammation and are a useful marker for improvement following continuous positive airway pressure therapy PMID: 27665460
    22. This study is the first to demonstrate an effect of HIV on the IL-23 signaling pathway in Th17 cells. We show that in vitro and in vivo HIV infection results in impaired IL-23 signaling which is not reversed by HAART nor is it a result of reduced receptor expression, suggesting that HIV interferes with IL-23-activated signaling pathways PMID: 29091911
    23. We conclude that variants in IL-23A and IL-23R genes were associated with the risk of multiple sclerosis or other inflammatory demyelinating diseases. PMID: 27893410
    24. results confirm the role of IFN-gamma and support the involvement of IL-23/Th17-related cytokines in BCC pathogenesis and in the inflammatory response during IMQ and MAL-PDT treatments PMID: 28829805
    25. An increase in CSF mean levels of IL-23 was observed in amyotrophic lateral sclerosis patients. PMID: 27245439
    26. results show that IL-23 accounts for the main aspects of human and murine lupus including the expansion of double negative T cells, decreased IL-2, and increased IL-17 production PMID: 28646040
    27. Serum Levels of IL-17A and IL-23 Are Associated with Decreased Vitamin D3 and Increased Pain in Osteoarthritis PMID: 27820818
    28. this study shows that latent cytomegalovirus induces IL-23. IL-23 might be an inflammatory mediator of latent cytomegalovirus infection in patients with rnd-stage renal diseases, and predisposes patients for post-transplant cytomegalovirus reactivation PMID: 27716059
    29. These finding may provide a molecular mechanism involved in sustained ER stress in AS BdCs stimulates the activation of RUNX2 and C/EBPbeta genes, leading to IL-23 production. PMID: 28728847
    30. The link between increased expression of TH17 pathway cytokines and clinical disease severity raises the possibility of a new therapeutic paradigm of targeted IL-17/IL-23 intervention for patients with ichthyosis PMID: 27554821
    31. this study shows that elevated iserum IL-23 is associated with mortality in chronic hepatitis B virus, acute-on-chronic liver failure patients, and that IL-23 neutralizing antibodies attenuated liver injury by reducing the expression of Th17-related inflammatory cytokines, neutrophil chemoattractants and stabilization factors within the liver tissue PMID: 28282579
    32. this study shows that IL-23 promotes IL-17+IFN-gamma- T cell expansion in multi-drug-resistant tuberculosis PMID: 27681197
    33. our results suggest that IL-39 induces differentiation and/or expansion of neutrophils in lupus-prone mice. PMID: 27400195
    34. The results of this case-control study suggest that IL-12A, IL-12B, IL12RB1, IL12RB2 and IL23R make no genetic contribution to the susceptibility of Takayasu arteritis in Chinese populations PMID: 26987707
    35. High IL-23 expression is associated with polycystic ovary syndrome. PMID: 27146815
    36. this study shows that for pre-school children with history and physical exam in favor of asthma which cannot be tested by spirometry, IL-23 serum levels may be an auxiliary biomarker for the diagnosis of asthma PMID: 28341820
    37. Data show that the number of interleukin 17 (IL-17) positive cells significantly correlated positively with the number of interleukin-23 (IL-23) positive cells in skin PMID: 27388654
    38. Interleukin 17A (Il17a) and interleukin-23 (IL-23) - dependent interleukin-17 Receptor C (IL-17RC) are expressed by sputum and neutrophils in deltaF508-CFTR protein (F508del) cystic fibrosis patients. PMID: 27155366
    39. suberoylanilide hydroxamic acid significantly inhibited IL-12p40 and IL-23p19 mRNA synthesis and did not change IL-12p35 mRNA transcription. PMID: 27240992
    40. this study shows that single nucleotide polymorphisms of the IL23R-IL12RB2 region are associated with Behcet's disease in a Northern Chinese Han population PMID: 27660093
    41. this study shows that double-negative T cells from systemic lupus erythematosus patients are more expanded when stimulated in vitro with recombinant IL-23 than controls PMID: 27343994
    42. Increased expression of interleukin-23 associated with progression of colorectal cancer. PMID: 27807862
    43. The levels of IL-17, one of the major effector cytokines of the Th17 system, and IL-23, which had been implicated in the generation, survival and expansion of Th17 cells, are altered in Hashimoto's thyroiditis (HT). PMID: 26928603
    44. Intracellular p19 associated with the cytokine receptor subunit gp130 and stimulated the gp130-dependent activation of signal transducer and activator of transcription 3 (STAT3) signaling PMID: 26980441
    45. may role in the pathogenesis of arthritis and may be a useful biomarker for the diagnosis of this disease PMID: 26709220
    46. we showed that elevated IL-17/IL-23 serum concentrations helped to discriminate BP patients who later relapsed. PMID: 26656739
    47. The expression of IL-23 significantly increased in chronic atrophic gastritis with intestinal metaplasia tissues and gastric cancer tissues. PMID: 27053620
    48. that consumption of star fruit juice at 100g twice daily for one month can significantly depress the pro-inflammation cytokines: TNF-alpha, IL-23, and NO, while increasing walking distance. PMID: 26952371
    49. IL-23 mediates the differentiation of Th17 and Treg cells and the production of associated cytokines in DICs. The potential mechanism likely involves the STAT3 pathway. PMID: 26657845
    50. Clostridium difficile toxins A and B induce IL-23 mRNA expression in human dendritic cells. PMID: 25626905

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  • 亚细胞定位：
    Secreted. Note=Secreted upon association with IL12B.
  • 蛋白家族：
    IL-6 superfamily
  • 组织特异性：
    Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level).
  • 数据库链接：

    HGNC: 15488

    OMIM: 605580

    KEGG: hsa:51561

    STRING: 9606.ENSP00000228534

    UniGene: Hs.382212