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  4. Recombinant Rat Neural cell adhesion molecule 1 (Ncam1), partial

Recombinant Rat Neural cell adhesion molecule 1 (Ncam1), partial

In Stock
  • 货号：
    CSB-EP015511RA
  • 规格：
    ￥1836
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ncam1; Ncam; Neural cell adhesion molecule 1; N-CAM-1; NCAM-1; CD antigen CD56
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Extracellular Domain
  • 来源：
    E.coli
  • 分子量：
    82.7kDa
  • 表达区域：
    20-721aa
  • 氨基酸序列
    LQVDIVPSQGEISVGESKFFLCQVAGDAKDKDISWFSPNGEKLSPNQQRISVVWNDDDSSTLTIYNANIDDAGIYKCVVTAEDGTQSEATVNVKIFQKLMFKNAPTPQEFKEGEDAVIVCDVVSSLPPTIIWKHKGRDVILKKDVRFIVLSNNYLQIRGIKKTDEGTYRCEGRILARGEINFKDIQVIVNVPPTVQARQSIVNATANLGQSVTLVCDADGFPEPTMSWTKDGEPIENEEEDDEKHIFSDDSSELTIRNVDKNDEAEYVCIAENKAGEQDASIHLKVFAKPKITYVENQTAMELEEQVTLTCEASGDPIPSITWRTSTRNISSEEKASWTRPEKQETLDGHMVVRSHARVSSLTLKSIQYTDAGEYICTASNTIGQDSQSMYLEVQYAPKLQGPVAVYTWEGNQVNITCEVFAYPSATISWFRDGQLLPSSNYSNIKIYNTPSASYLEVTPDSENDFGNYNCTAVNRIGQESLEFILVQADTPSSPSIDRVEPYSSTAQVQFDEPEATGGVPILKYKAEWKSLGEEAWHSKWYDAKEANMEGIVTIMGLKPETRYAVRLAALNGKGLGEISAATEFKTQPVREPSAPKLEGQMGEDGNSIKVNLIKQDDGGSPIRHYLVKYRALASEWKPEIRLPSGSDHVMLKSLDWNAEYEVYVVAENQQGKSKAAHFVFRTSAQPTAIPANGSPTAGLST
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    This Recombinant Rat Ncam1 protein was made through genetic engineering. By putting the Ncam1 gene into the genetic material of E.coli cell, the E.coli could be used as factories or producers to make the desired Ncam1 protein for research uses. The expression region of this protein is at 20-721aa. N-terminal 10xHis tag and C-terminal Myc tag was used in the expression process. The purity is 90%+ determined by SDS-PAGE.

    NCAM1 belongs to the immunoglobulin superfamily of adhesion molecules. 5 A wide range of alternatively spliced NCAM1 messenger RNAs (mRNAs) has been described to date, but only the 120-, 140-, and 180- kDa isoforms are commonly expressed. NCAM1 plays an important role in the regulation of neurogenesis, neurite outgrowth, proliferation, and cell migration, however, its function in hematopoiesis, including NK cells, is poorly understood. NCAM1 signaling is mediated either by homophilic or heterophilic interactions with fibroblast growth factor receptor (FGFR), L1-CAM, N-cadherin and other components of the extracellular matrix. Upon activation, NCAM1 triggers a variety of signaling cascades including FYN–focal adhesion kinase (FAK), MAPK, and phosphatidylinositol 3-kinase (PI3K) pathways. NCAM1 represents the major isoform expressed in cancer cells and has also been described in several tumor entities such as glioma, ovarian cancer, and small cell lung cancer. Functionally, NCAM1 expression is linked to disease progression.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • 基因功能参考文献：
    1. Our results strongly support the hypothesis that PSA-NCAMs play a proaggregatory role in the fasciculatory process PMID: 23963795
    2. CD56(140kD) up-regulation plays a pivotal role in the pathogenesis of ischemic cardiomyopathy. PMID: 23462508
    3. Stressed animals exhibited an increase in NCAM mRNA expression in the hippocampus, but not in the prefrontal cortex. The synaptosomal NCAM of the hippocampus was markedly polysialylated, while cortical PSA-NCAM was significantly decreased. PMID: 22814229
    4. A serine-to-alanine exchange at position 774 of NCAM resulted in decreased activation of the cAMP response element binding protein and decreased neurite outgrowth after NCAM stimulation in neuroblastoma cells PMID: 22419107
    5. FGL triggers a long-lasting enhancement of synaptic transmission and long-term potentiation in hippocampal CA1 neurons by a facilitated synaptic delivery of AMPA receptors. PMID: 22363206
    6. NCAM-derived peptide plannexin can protect immature neurons in vivo; however, in a model of status epilepticus plannexin-treated animals do not reveal any neuroprotective effects of plannexin on mature neurons. PMID: 21787839
    7. Treatment with triiodothyronine downregulates expression of NCAM in the seminiferous tubules of rats with flutamide-induced cryptorchidism. PMID: 22007488
    8. Polysialylated-NCAM expression is important for the connectivity of interneurons in the adult cerebral cortex; its regulation may play an important role in the structural plasticity of inhibitory networks. PMID: 20843898
    9. Plannexin facilitates neurite outgrowth in primary hippocampal neuronal cultures and improves spatial learning under conditions involving a deficit in a key plasticity-promoting posttranslational modification of NCAM, its polysialylation. PMID: 21887252
    10. We suggest that the expression pattern of various FGFR isoforms determines the cell context-specific effects of NCAM signaling PMID: 21876469
    11. N-CAM is influenced by an intentionally misdirected outgrowth of motor and sensory axons following nerve repair. PMID: 20622691
    12. NCAM is a cardioprotective factor up-regulated under metabolic stress in cardiomyocytes PMID: 19853610
    13. Results indicate that the phosphorylation-dependent interaction between 14-3-3beta and alphaII spectrin acts as a switch between positive and negative regulation of neurite outgrowth stimulated by NCAM. PMID: 20598904
    14. a decisive role for the neuronal K(+) channel in regulating NCAM-dependent neurite outgrowth and attribute a physiologically meaningful role to the functional interplay of Kir3.3, NCAM, and TrkB in ontogeny PMID: 20610389
    15. Kidney cell proliferation, assessed by BrdU and PCNA labeling, was prominent in outer medulla and reach a maximum between 24 and 72 hours after reperfusion. This population was characterized by the coexpression of vimentin and nestin. PMID: 20617137
    16. the activation of FRS2alpha by FGL was significantly lower than the activation by FGF1, indicating a differential signaling profile induced by NCAM compared with the cognate growth factor PMID: 20175207
    17. results suggest a positive role of NCAM in neurogenesis in the periventricular region; expression of NCAM in stem cells might be one of many factors useful for therapeutic approaches in the future PMID: 19788570
    18. microdomains responsive to NCAM-mediated signaling from growth cones. PMID: 11817895
    19. distribution of the NCAM immunoreactivity in the sciatic nerves of 10- and 15-day-old Wistar rats PMID: 11936192
    20. Reciprocal actions of neural cell adhesion molecule and tissue plasminogen activator via a Ras-dependent mitogen-activated protein kinase signaling pathway in hippocampal neurons. PMID: 12387826
    21. The effect of exposure to restraint stress on NCAM mRNA was evaluated in the hippocampus. A decrease in NCAM mRNA was found throughout all hippocampal subregions. Stress may thus induce structural alterations in the the hippocampus. PMID: 12453492
    22. Integrin alphav and NCAM mediate the effects of GDNF on DA neuron survival, outgrowth, DA turnover and motor activity in rats. PMID: 12493556
    23. Polysialic acid NCAM expression appears first in the developing inner hair cells (IHCs) of the cochlea and then in the neighboring Kolliker's organ in an increasingly mediolateral gradient. PMID: 12640664
    24. results showed that the protein concentration of the NCAM-140 isoform decreased in the hippocampus of stressed rats PMID: 12757368
    25. Loss or inactivation of the polysialyl tail of NCAM reduces the migration of mechanically wounded oligodendrocyte progenitor-like (O-2A) cells into the injured area and completely blocks the wound closure. PMID: 12774308
    26. Removal of PSA from NCAM by the enzyme Endo N leads to a significant loss of both VP and OT neurons PMID: 12786978
    27. direct interaction between NCAM and fibroblast growth factor receptor 1 (Ig modules 2 and 3) by surface plasmon resonance analysis PMID: 12791257
    28. Association of NCAM with GFRalpha1 downregulates NCAM-mediated cell adhesion and promotes GDNF binding to NCAM, resulting in rapid activation of cytoplasmic protein tyrosine kinases Fyn and FAK. PMID: 12837245
    29. Strong overexpression of NCAM(CD56) and RUNX1(AML1) is a constant and characteristic feature of cardiomyocytes within or adjacent to scars in ICM. PMID: 12937148
    30. Traumatic fear conditioning suppresses NCAM-PSA-mediated plasticity and the concomitant inability to store the trace of recently acquired information. PMID: 13129654
    31. neural cell adhesion molecule is associated with major components of the cytoskeleton PMID: 14550299
    32. The most intense polysialylated NCAM immunoreactivity is localized in the medial and triangular septal nuclei, regions of the brain that regulate hippocampal theta rhythm during memory consolidation. PMID: 14648585
    33. demonstrated for the first time that IUGR newborn rat kidneys express N-CAM and cadherin adhesion molecules at specific sites of the nephron. PMID: 14663363
    34. Amygdaloid-kindling increases polysialylated NCAM-immunoreactive mossy fibers and their terminals in the stratum lucidum of the hippocampal CA3 region and in the granule cells of the dentate gyrus. PMID: 14741393
    35. The circulating count of CD56(dim) natural killer cells does not change during exercise training. PMID: 14752119
    36. 12 h posttraining, immunohistochemical analyses revealed a learning-induced up-regulation of polysialylated neural cell adhesion molecule in the dentate gyrus related to the spatial learning abilities displayed by rats during training. PMID: 15169853
    37. Relevance of NCAM for tumor cell binding was proven by treating neuroblastoma cells with NCAM antisense oligonucleotides or NCAM transfection. PMID: 15256054
    38. We therefore suggest that early synaptic NCAM expression may be one of the factors determining the capability of rats to learn. PMID: 15490465
    39. Several interacting partners of cytosolic domains of rat brain neural cell adhesion molecule (NCAM) isoforms 140 and 180 have been identified that may play a role in NCAM-mediated signal transduction. PMID: 15865439
    40. Results suggested that the expressions of NCAM, M-cadherin and beta-catenin in skeletal muscle satellite cells may be controlled by distinct regulatory mechanisms during functional overload and muscle hypertrophy. PMID: 16776666
    41. Decreased NCAM-1 expression in CA1 and CA3 fields of the hippocampus, and in a lesser degree in the basal ganglia, limbic structure and cervical spinal cord in autoimmune encephalitis PMID: 17064783
    42. upregulation of NCAM is a correlate of fear conditioning that is not necessary for the establishment of fear memory in the amygdala, but participates in mechanisms precluding fear extinction PMID: 17223582
    43. These findings show evidence of a selective neuronal expression of PSA-NCAM in developing thalamus, supporting its suggested role in cell migration and synaptogenesis as it occurs in the cerebral cortex PMID: 17292800
    44. The upregulation of PSA-NCAM by hyperthermia may have a significant impact on hippocampal plasticity, permitting induction of the complex molecular cascade responsible for neuroprotection. PMID: 17336079
    45. After a dose of a CB1 receptor agonist the number of PSA-NCAM immunoreactive (IR) cells in the dentate gyrus declined in a time-dependent manner. PMID: 17355876
    46. Exposure to stressors during juvenility alters NCAM expression and potentially underlies the predisposition to exhibit stress-related symptoms in adulthood. PMID: 17429411
    47. upregulation of PSA-NCAM expression in the dorsal (but not ventral) dentate gyrus at 24 h after contextual (but not tone) fear conditioning PMID: 17460068
    48. modulation of PSA-NCAM function may promote neuroregeneration in different central nervous system insults PMID: 17513116
    49. Fluoride causes oxidative stress, apoptosis, and decreased mRNA and protein expression levels of NCAM in rat hippocampal neurons. PMID: 17537562
    50. Given the role of PSA-NCAM in neuronal plasticity, the present results indicate that, even during aging, many regions of the CNS may display neurite, spine or synaptic remodeling. PMID: 17904697

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein.
  • 数据库链接：

    KEGG: rno:24586

    STRING: 10116.ENSRNOP00000048442

    UniGene: Rn.11283