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  4. Recombinant Pseudomonas aeruginosa Regulatory protein RhlR (rhlR)

Recombinant Pseudomonas aeruginosa Regulatory protein RhlR (rhlR)

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  • 货号：
    CSB-EP346531EZX
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP346531EZX could indicate that this peptide derived from E.coli-expressed Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) rhlR.
    • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP346531EZX could indicate that this peptide derived from E.coli-expressed Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) rhlR.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
    rhlR
  • Uniprot No.：
  • 别名：
    rhlR; lasM; vsmR; PA3477; Regulatory protein RhlR; Elastase modulator
  • 种属：
    Pseudomonas aeruginosa
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    47.6kDa
  • 表达区域：
    1-241aa
  • 氨基酸序列
    MRNDGGFLLWWDGLRSEMQPIHDSQGVFAVLEKEVRRLGFDYYAYGVRHTIPFTRPKTEVHGTYPKAWLERYQMQNYGAVDPAILNGLRSSEMVVWSDSLFDQSRMLWNEARDWGLCVGATLPIRAPNNLLSVLSVARDQQNISSFEREEIRLRLRCMIELLTQKLTDLEHPMLMSNPVCLSHREREILQWTADGKSSGEIAIILSISESTVNFHHKNIQKKFDAPNKTLAAAYAAALGLI
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    The DNA fragment encoding the 1-241aa of the Pseudomonas aeruginosa rhlR protein was fused with N-terminal 10xHis-SUMO tag and C-terminal Myc tag gene and then was inserted into the expression vector, which was subsequently transformed into the E.coli for expression. The resulting product was further purified to obtain the recombinant Pseudomonas aeruginosa rhlR protein. The purity of this recombinant rhlR protein is greater than 90% assessed by Bandscan software analysis combined with SDS-PAGE. This recombinant rhlR protein showed a band on the gel with a molecular weight of approximately 45 kDa.

    rhlR is a gene encoding a protein named regulatory protein RhlR and belongs autoinducer-regulated transcriptional regulatory protein family.This protein is necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. Regulation of gene expression plays a key role in bacterial adaptability to changes in the environment. In P. aeruginosa,quorum sensing (QS) relies on the activation of specific transcriptional regulators (LasR and RhlR) by corresponding signals synthesized by their cognate acyl HSL synthases (LasI and RhlI). rhlR transcription is subject to positive-feedback autoregulation through RhlR/C4-HSL activation of the rhlA promoter.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Necessary for transcriptional activation of the rhlAB genes encoding the rhamnosyltransferase. It also functions as a transcriptional activator of elastase structural gene (lasB). Binds to autoinducer molecules BHL (N-butanoyl-L-homoserine lactone), and HHL (N-hexanoyl-L-homoserine lactone).
  • 基因功能参考文献：
    1. this work shows that PqsE and RhlR function as a QS-autoinducer synthase-receptor pair that drives group behaviors in P. aeruginosa. PMID: 30224496
    2. rhlR/lasR knockout increased the susceptibility of Pseudomonas aeruginosa towards polymyxin B-azithromycin combination via influencing quorum sensing. PMID: 28096154
    3. This is the first time that a domain of the quorum sensing regulator RhlR was produced in sufficient amounts for structural studies, enabling the investigation of the molecular basis for RhlR specific interaction with DNA promoters. PMID: 26792557
    4. Quorum sensing genes lasR and rhlR and activity of electric field sensitive enzyme, glycerol-3-phosphate dehydrogenase was also repressed by WED. PMID: 25803639
    5. Subinhibitory concentrations of macrolides specifically upregulated expression of quorum sensing genes (rhlR, lasI), whereas sulfonamides and municipal wastewater, instead upregulated expression of specific resistant genes (sul1) and efflux pumps (mexD). PMID: 23392972
    6. The rmlBDAC operon has three promoters, and that the expression of the P2 promoter is dependent on the stationary phase sigma factor sigmaS and on the quorum sensing regulator RhlR. PMID: 22262098
    7. rhlR transcription is subject to positive-feedback autoregulation through RhlR/C4-HSL activation of the rhlA promoter. PMID: 21719541
    8. RhlR and LasR regulate the anthranilate metabolism in a mutually antagonistic and growth phase-differential manner. PMID: 21614486
    9. Analysis of rpoS gene and the rhlR gene per cell showed that P. aeruginosa cells express different proteins according to their location in the biofilm. PMID: 20348255
    10. MvfR contributes to P. aeruginosa virulence by controlling the transcription of genes not under RhlR regulation. PMID: 15686549
    11. down-regulation of the T3S regulon in the presence of RhlR-C4HSL and the corresponding advanced secretion of ExoS in a rhlI mutant. PMID: 15901720
    12. Data found that lon-disrupted cells show increased levels of a transcriptional regulator, RhlR. PMID: 18408026
    13. RhlR is able to induce LasR-regulated genes in the absence of lasR. PMID: 19246742

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  • 蛋白家族：
    Autoinducer-regulated transcriptional regulatory protein family
  • 数据库链接：

    KEGG: pae:PA3477

    STRING: 208964.PA3477