Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Influenza B virus Nucleoprotein (NP)

Recombinant Influenza B virus Nucleoprotein (NP)

In Stock
  • 货号：
    CSB-BP361330IJK
  • 规格：
    ￥3168
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    NP
  • Uniprot No.：
  • 别名：
    NP; Nucleoprotein; Nucleocapsid protein; Protein N
  • 种属：
    Influenza B virus (strain B/Lee/1940)
  • 蛋白长度：
    Full Length
  • 来源：
    Baculovirus
  • 分子量：
    64.3kDa
  • 表达区域：
    1-560aa
  • 氨基酸序列
    MSNMDIDSINTGTIDKTPEELTPGTSGATRPIIKPATLAPPSNKRTRNPSPERTTTSSETDIGRKIQKKQTPTEIKKSVYKMVVKLGEFYNQMMVKAGLNDDMERNLIQNAQAVERILLAATDDKKTEYQKKRNARDVKEGKEEIDHNKTGGTFYKMVRDDKTIYFSPIKITFLKEEVKTMYKTTMGSDGFSGLNHIMIGHSQMNDVCFQRSKGLKRVGLDPSLISTFAGSTLPRRSGTTGVAIKGGGTLVDEAIRFIGRAMADRGLLRDIKAKTAYEKILLNLKNKCSAPQQKALVDQVIGSRNPGIADIEDLTLLARSMVVVRPSVASKVVLPISIYAKIPQLGFNTEEYSMVGYEAMALYNMATPVSILRMGDDAKDKSQLFFMSCFGAAYEDLRVLSALTGTEFKPRSALKCKGFHVPAKEQVEGMGAALMSIKLQFWAPMTRSGGNEVSGEGGSGQISCSPVFAVERPIALSKQAVRRMLSMNVEGRDADVKGNLLKMMNDSMAKKTSGNAFIGKKMFQISDKNKVNPIEIPIKQTIPNFFFGRDTAEDYDDLDY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Tris-based buffer,50% glycerol
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：

    Intact influenza B virus nucleoprotein (NP) cDNA (1-560aa) with an N-terminal 10xHis-tag was expressed in the Baculovirus cells. The forming protein is the recombinant full-length influenza B virus NP protein. The purity of this protein is greater than 85% determined by SDS-PAGE. Under reducing conditions, the gel showed a molecular weight band of about 65 kDa. In-stock influenza B virus NP proteins are offered now. In addition to producing specific antibodies, this recombinant influenza B virus NP protein may be used in the studies of microbiology.

    Nucleoprotein (NP) is the predominant component of the influenza virus ribonucleoprotein. It is highly conserved among each type of influenza viruses. Alice Labaronne etc. demonstrated that the NP of influenza B virus has a long N-terminal tail of 70 residues with intrinsic flexibility. This tail contains the Nuclear Location Signal (NLS). And NP is imported into the nucleus by the importin-α/β pathway via direct interaction with importin-α isoforms. In addition, NP is the potential target for a universal influenza vaccine.

  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha/beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1/XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.
  • 基因功能参考文献：
    1. Results show that the N terminus is involved in the formation of both higher homo-oligomers of BNP and BNP-RNA complex. PMID: 26368391
  • 亚细胞定位：
    Virion. Host nucleus.
  • 蛋白家族：
    Influenza viruses nucleoprotein family
  • 数据库链接：

    KEGG: vg:26824002