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Human Immunoglobulin A,IgA ELISA Kit

  • 中文名称:
    人免疫球蛋白A(IgA)酶联免疫试剂盒
  • 货号:
    CSB-E07985h
  • 规格:
    96T/48T
  • 价格:
    ¥3600/¥2500
  • 其他:

产品详情

  • 产品描述:
        免疫球蛋白A(Immunoglobulin A,IgA)是免疫球蛋白家族的一种,是人体中分布最广泛的免疫球蛋白。它主要存在于体液中,如唾液、乳汁、泪液、鼻涕、肠液等,能够通过黏膜免疫屏障对机体进行保护。IgA具有免疫调节和免疫防御的作用,其水平变化与许多免疫相关的疾病,如感染、炎症、肿瘤等密切相关。
        j9九游会登录入口首页生物所提供的Human Immunoglobulin A,IgA ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆样本中的IgA,其灵敏度为0.24 ng/mL,检测范围为0.24 ng/mL-1000 ng/mL。
     
  • 别名:
    N/A
  • 缩写:
    IgA
  • 种属:
    Homo sapiens (Human)
  • 样本类型:
    serum, plasma
  • 检测范围:
    0.24 ng/mL-1000 ng/mL
  • 灵敏度:
    0.24 ng/mL
  • 反应时间:
    1-5h
  • 样本体积:
    50-100ul
  • 检测波长:
    450 nm
  • 研究领域:
    Immunology
  • 测定原理:
    quantitative
  • 测定方法:
    Sandwich
  • 精密度:
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度:
    To assess the linearity of the assay, samples were spiked with high concentrations of human IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:10000 Average % 89  
    Range % 85-94  
    1:20000 Average % 90  
    Range % 86-97  
    1:40000 Average % 96  
    Range % 90-103  
    1:80000 Average % 89  
    Range % 86-93  
  • 回收率:
    The recovery of human IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 91 89-94  
    EDTA plasma (n=4) 92 88-97  
                 
                 
  • 标准曲线:
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average Corrected  
    1000 2.180 2.205 2.193 2.124  
    250 2.093 2.002 2.048 1.979  
    62.5 1.841 1.868 1.855 1.786  
    15.6 1.528 1.501 1.515 1.446  
    3.9 0.772 0.753 0.763 0.694  
    0.98 0.396 0.397 0.397 0.328  
    0.24 0.189 0.180 0.185 0.116  
    0 0.069 0.068 0.069    
  • 数据处理:
  • 货期:
    3-5 working days

产品评价

靶点详情

  • 最新研究进展:
    IgA(免疫球蛋白A)是一种免疫球蛋白,分布于人体内许多黏膜表面和体液中。IgA是黏膜免疫的主要保护因子,主要通过抗体中和病原体和调节肠道微生物群维持肠道健康。此外,IgA还在妊娠期间保护胎儿免受感染,参与自身免疫调节,发挥重要的生物学作用。