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  4. Pig Immunoglobulin A,IgA ELISA Kit

Pig Immunoglobulin A,IgA ELISA Kit

  • 中文名称：
    猪免疫球蛋白A(IgA)酶联免疫试剂盒
  • 货号：
    CSB-E13234p
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：

    This Pig IgA ELISA Kit was designed for the quantitative measurement of Pig IgA protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.146 μg/mL-37.5 μg/mL and the sensitivity is 0.146 μg/mL.

  • 缩写：
    IgA
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.146 μg/mL-37.5 μg/mL
  • 灵敏度：
    0.146 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1000 Average % 92  
    Range % 88-94  
    1:2000 Average % 93  
    Range % 90-98  
    1:4000 Average % 88  
    Range % 85-94  
    1:8000 Average % 95  
    Range % 91-100  
  • 回收率：
    The recovery of pig IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 94 89-97  
    EDTA plasma (n=4) 103 98-106  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    37.5 0.199 0.209 0.204    
    9.375 0.445 0.420 0.433    
    2.345 0.924 0.955 0.940    
    0.586 1.413 1.447 1.430    
    0.146 2.101 2.114 2.108    
    0 2.547 2.503 2.525    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：其他 猪

稀释比：1:100

产品评价： 标曲准确，样本数值在标曲范围之内，符合预期结果。

By 谢老师

问答及客户评论

■ 常见问题解答
Q:

Hi. I would like to know if this kit is used feces samples from pig. If so, I would like knowing the cost. Also I would like to know if you have avilable the kit with reagents for plate preparation and its respective cost, as well. Thanks much.

A:
Thanks for your inquiry.
For IgA, there are serotype and secretory type. If you want to test feces samples, we recommend you choose Pig secretory immunoglobulin A,SIgA ELISA KitCSB-E12063p. No matter you use CSB-E12063p or CSB-E13234p, we suggest you do a pretest first.
All the components in the kit are well prepared, so you don't need to prepare the reagents by yourself. Sorry we can not provide reagents for plate preparation.Pls let me know if you have any further questions. Thank you.