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  4. Fish immunoglobulin M (IgM) ELISA Kit

Fish immunoglobulin M (IgM) ELISA Kit

  • 中文名称：
    鱼免疫球蛋白IgM酶联免疫试剂盒
  • 货号：
    CSB-E12045Fh
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：

产品详情

  • 产品描述：
        IgM（免疫球蛋白M）是一种血液中存在的抗体类型，通常出现在初次感染和特定疾病后。
        j9九游会登录入口首页生物所提供的Fish immunoglobulin M (IgM) ELISA Kit属于ELISA检测试剂盒，采用竞争法定量检测鱼类血清、血浆、组织匀浆样本中的IgM，其灵敏度为1.25 μg/mL，检测范围为1.25 μg/mL-50 μg/mL。
     
  • 缩写：
    IgM
  • 种属：
    Fish
  • 样本类型：
    serum, plasma, tissue homogenates
  • 检测范围：
    1.25 μg/mL-50 μg/mL
  • 灵敏度：
    1.25 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<15%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<15%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of fish IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
    　 Sample Serum(n=4)  
    1:1 Average % 93  
    Range % 86-101  
    1:2 Average % 97  
    Range % 90-104  
    1:4 Average % 95  
    Range % 84-105  
    1:8 Average % 95  
    Range % 91-99  
  • 回收率：
    The recovery of fish IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 97 92-105  
    EDTA plasma (n=4) 95 91-102  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    50 0.122 0.124 0.123    
    25 0.213 0.199 0.206    
    11.5 0.401 0.427 0.414    
    3.75 0.745 0.761 0.753    
    1.25 1.375 1.402 1.389    
    0 2.541 2.532 2.537    
  • 本试剂盒所含材料：
      • A 96-well Assay plate --The 96-well plate has been pre-coated with goat-anti-rabbit antibody.
      • Standard (6 x 0.5 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
      • HRP-conjugated IgM (1 x 6 ml) --Bind to the IgM antibody, and HRP catalyzes the TMB to elicit a chromogenic reaction.
      • IgM Antibody (1 x 6 ml) --Interact with the IgM.
      • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
      • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
      • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
      • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
      • Four Adhesive Strips (For 96 wells)
      • An Instruction manual

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  • 本试剂盒不含材料：
      • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
      • An incubator that can provide stable incubation conditions up to 37°C±5°
      • Centrifuge
      • Vortex
      • Squirt bottle, manifold dispenser, or automated microplate washer
      • Absorbent paper for blotting the microtiter plate
      • 50-300ul multi-channel micropipette
      • Pipette tips
      • Single-channel micropipette with different ranges
      • 100ml and 500ml graduated cylinders
      • Deionized or distilled water
      • Timer
      • Test tubes for dilution

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  • 数据处理：
  • 货期：
    3-5 working days

引用文献

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问答及客户评论

■ 常见问题解答
Q:

CUSABIO fish immnoglobulin M (IgM) ELISA kit Does this kit can be used for separate experiments?around 30-40 wells every time?

A:
Thanks for your inquiry.
#CSB-E12045Fh,Fish immunoglobulin M (IgM) ELISA Kit
YES,it can.The plate is Breakapart and can be used for seperate experiments.You can put the break part in the
Aluminum foil bag for next use.The manual is provided to you by e-mail.Additionally,we received 21 published papers until now.You can refer to it. Pls let me know if you have any further questions. Thank you.
Q:

Regarding your ELISA kit CSB-E12045Fh, could you please advise on the following:
1. Capture Antibody: host animal species, clonality
2. Detection Antibody: host animal species, clonality
3. Standard
Also, what sample types have been tested with this kit, and what were the reference values?

A:
Thanks for your inquiry.
CSB-E12045Fh
The target is a special protein and there is no uniprot.
This kit is based on competitive method. The plate is coated with goat-anti-rabbit antibody. The specific antibody is rabbit polyclonal antibody. The standard is purified crude extract of natural samples.
We tested undiluted serum and plasma before and the test value is 1.25--3.75μg/ml.
Pls let me know if you have any further questions. Thank you.

靶点详情

  • 最新研究进展：
    IgM是体液免疫系统中最早产生的抗体，通常作为感染初期的主要抗体。最新研究表明，IgM除了参与传统的抗体介导的免疫反应外，还具有其他生物学功能，如参与调节免疫细胞的增殖和凋亡等。此外，研究人员正在探索利用IgM抗体治疗感染和自身免疫性疾病等疾病的潜在机制和应用前景。