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  4. Pig Immunoglobulin M,IgM ELISA Kit

Pig Immunoglobulin M,IgM ELISA Kit

  • 中文名称：
    猪免疫球蛋白M(IgM)酶联免疫试剂盒
  • 货号：
    CSB-E06805p
  • 规格：
    96T/48T
  • 价格：
    ￥3800/￥2500
  • 其他：

产品详情

  • 产品描述：

    This Pig IgM ELISA Kit was designed for the quantitative measurement of Pig IgM protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.039 μg/mL-10 μg/mL and the sensitivity is 0.039 μg/mL.

  • 缩写：
    IgM
  • 种属：
    Sus scrofa (Pig)
  • 样本类型：
    serum, plasma
  • 检测范围：
    0.039 μg/mL-10 μg/mL
  • 灵敏度：
    0.039 μg/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Immunology
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of pig IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1 Average % 97  
    Range % 92-103  
    1:2 Average % 87  
    Range % 81-92  
    1:4 Average % 95  
    Range % 90-99  
    1:8 Average % 102  
    Range % 96-108  
  • 回收率：
    The recovery of pig IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 96 91-102  
    EDTA plasma (n=4) 104 98-108  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    μg/ml OD1 OD2 Average    
    10 0.204 0.206 0.205    
    2.5 0.463 0.454 0.459    
    0.62 0.915 0.936 0.926    
    0.16 1.429 1.488 1.459    
    0.04 2.056 2.134 2.095    
    0 2.515 2.652 2.584    
  • 数据处理：
  • 货期：
    3-5 working days

引用文献

产品评价

平均分:
5.0分 - 1 个评价

样品类型：血清

样品信息：其他 猪

稀释比：没有稀释

产品评价： 标曲准确，样本数值在标曲范围之内，符合预期结果。

By 谢老师