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  4. Recombinant Rat Sphingosine 1-phosphate receptor 2 (S1pr2)

Recombinant Rat Sphingosine 1-phosphate receptor 2 (S1pr2)

  • 货号：
    CSB-CF020651RA
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    41.6 kDa
  • 表达区域：
    1-352aa
  • 氨基酸序列
    MGGLYSEYLNPEKVQEHYNYTKETLDMQETPSRKVASAFIIILCCAIVVENLLVLIAVARNSKFHSAMYLFLGNLAASDLLAGVAFVANTLLSGPVTLSLTPLQWFAREGSAFITLSASVFSLLAIAIERQVAIAKVKLYGSDKSCRMLMLIGASWLISLILGGLPILGWNCLDHLEACSTVLPLYAKHYVLCVVTIFSVILLAIVALYVRIYFVVRSSHADVAGPQTLALLKTVTIVLGVFIICWLPAFSILLLDSTCPVRACPVLYKAHYFFAFATLNSLLNPVIYTWRSRDLRREVLRPLLCWRQGKGATGRRGGNPGHRLLPLRSSSSLERGLHMPTSPTFLEGNTVV
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    C-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor for the lysosphingolipid sphingosine 1-phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effects on most types of cells and tissues. Receptor for the chemokine-like protein FAM19A5. Mediates the inhibitory effect of FAM19A5 on vascular smooth muscle cell proliferation and migration.
  • 基因功能参考文献：
    1. Sphingosine-1 phosphate (S1P)-mediated activation of S1PR2 plays an important role in regulating intestinal epithelial cell proliferation and migration. S1P significantly upregulated the expression of c-Myc, cyclin D1, E-cadherin and zona occluden-1 (ZO-1), which was completely inhibited by downregulation of S1PR2 expression with a shRNA. PMID: 27814635
    2. TGR5 activation reduced ICAM-1, TGF-beta1 and FN expressions induced by high glucose in glomerular mesangial cells, the mechanism might be through suppressing S1P/S1P2 signaling, thus ameliorating diabetic nephropathy. PMID: 27317945
    3. S1P contracted control colon via S1PR1 and S1PR2 and inflamed colon exclusively via S1PR2. Calcium influx (control) or release (inflamed) and calcium sensitization are involved in S1P-induced contraction. Exacerbated response to S1P in colitic colon segments may explain altered colonic motility reported in patients and experimental models of inflammatory bowel disease. PMID: 28493876
    4. Inhibition of S1PR2 down-regulate the expressions of SphK1 and MCP-1 in glomerular mesangial cells under high glucose. PMID: 25575056
    5. Conjugated bile acids promote cholangiocarcinoma growth through S1PR2. PMID: 24700501
    6. extracellular S1P induces COX-2 expression via activation of S1P2 and subsequent Gi and p42/p44 MAPK-dependent signaling in renal mesangial cells leading to enhanced PGE2 formation and cell migration that essentially requires COX-2. PMID: 24064301
    7. S1P may play an important role in the pathophysiology of portal hypertension with Rho kinase activation by way of S1P(2) . PMID: 22505286
    8. Results indicate that the impaired functions (such as chemotactic, wound healing, and morphogenetic responses) in senescent endothelial cells are mediated by the S1P(2) receptor. PMID: 22139303
    9. S1P2 receptor was significantly up-regulated under diabetic condition. S1P2 receptor mediated fibronectin expression through the activation of S1P-S1P2-MAPK axis in mesangial cells under high glucose condition. PMID: 22406263
    10. Conjugated bile acids activate the ERK1/2 and AKT signaling pathways primarily through S1P(2) in primary rodent hepatocytes. PMID: 21932398
    11. S1P modulates central intracellular signaling cascades and is antiproliferative in PC Cl(3) cells. S1P(2) receptor expression is modulated by insulin and TSH, two central growth factors in thyroid cell regulation. PMID: 15713536
    12. S1P (sphingosine-1-phosphate receptor)1 and S1P3 receptors promote, whereas S1P2 receptors antagonize, smooth muscle cell proliferation and phenotypic modulation in vitro in response to sphingosine-1-phosphate (S1P), or in vivo after vascular injury PMID: 18535287
    13. Data demonstrate the importance of S1P and the S1P(2) receptor as regulators of allergen-induced activation of mast cells in their natural environment in the rat lung. PMID: 19636535

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Expressed in all developing tissues with highest levels detected in primitive, transformed cells. Relative abundance: lung > kidney = skin = gut > spleen > brain > liver.
  • 数据库链接：

    KEGG: rno:29415

    STRING: 10116.ENSRNOP00000028034

    UniGene: Rn.2491