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  4. Recombinant Mouse Nicastrin (Ncstn)

Recombinant Mouse Nicastrin (Ncstn)

  • 货号：
    CSB-CF015600MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Ncstn; Nicastrin
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    28-708
  • 氨基酸序列
    GLCGGNSVERKIYIPLNKTAPCVRLLNATHQIGCQSSISGDTGVIHVVEKEEDLKWVLTDGPNPPYMVLLEGKLFTRDVMEKLKGTTSRIAGLAVTLAKPNSTSSFSPSVQCPNDGFGIYSNSYGPEFAHCKKTLWNELGNGLAYEDFSFPIFLLEDENETKVIKQCYQDHNLGQNGSAPSFPLCAMQLFSHMHAVISTATCMRRSFIQSTFSINPEIVCDPLSDYNVWSMLKPINTSVGLEPDVRVVVAATRLDSRSFFWNVAPGAESAVASFVTQLAAAEALHKAPDVTTLSRNVMFVFFQGETFDYIGSSRMVYDMENGKFPVRLENIDSFVELGQVALRTSLDLWMHTDPMSQKNESVKNQVEDLLATLEKSGAGVPEVVLRRLAQSQALPPSSLQRFLRARNISGVVLADHSGSFHNRYYQSIYDTAENINVTYPEWQSPEEDLNFVTDTAKALANVATVLARALYELAGGTNFSSSIQADPQTVTRLLYGFLVRANNSWFQSILKHDLRSYLDDRPLQHYIAVSSPTNTTYVVQYALANLTGKATNLTREQCQDPSKVPNESKDLYEYSWVQGPWNSNRTERLPQCVRSTVRLARALSPAFELSQWSSTEYSTWAESRWKDIQARIFLIASKELEFITLIVGFSTLVFSLIVTYCINAKADVLFVAPREPGAVSY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). The gamma-secretase complex plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels.
  • 基因功能参考文献：
    1. mice defective of the nicastrin subunit of gamma-secretase in oligodendrocytes have hypomyelination in the central nervous system. PMID: 27008863
    2. The results of this study showed that elevated p-tau levels in NCT cKO mice and have demonstrated that changes on p-tau were likely caused by enhanced activity of CDK5 but not GSK3beta. PMID: 26804055
    3. PS1(exon8) interacts with nicastrin, participating in the gamma-secretase complex formation. PMID: 26608390
    4. nicastrin plays essential roles in the regulation of short- and long-term synaptic plasticity, highlighting the importance of gamma-secretase in the function of mature synapses PMID: 24889619
    5. These results reveal a key role for ncstn in modulating amyloid beta production and amyloid plaque formation. PMID: 23826707
    6. Upregulation of PS1/gamma-secretase activity may be a risk factor for late onset sporadic Alzheimer's disease. PMID: 22140537
    7. Pen-2, as well as nicastrin and Aph-1alpha, is dispensable for presenilin endoproteolysis PMID: 22973949
    8. We propose a model that identifies critical TMDs of Aph-1 for associations with Nct and PS for the stepwise assembly of gamma-secretase components. PMID: 20382452
    9. Down-regulation of the ATP-binding cassette transporter 2 (Abca2) reduces amyloid-beta production by altering Nicastrin maturation and intracellular localization. PMID: 22086926
    10. we found gamma-secretase components, including nicastrin, to cleave BCG-derived Ag85B to produce a peptide epitope PMID: 22039303
    11. single residues in a gamma-secretase component besides presenilin are able to differentially affect amyloid precursor protein and Notch processing. PMID: 21768095
    12. increased NADH levels resulting from ENOX2 inhibition result in decreased prosurvival sphingosine-1-phosphate and increased proapoptotic ceramide, both of which may be important to initiation of the ENOX2 inhibitor-induced apoptotic cascade. PMID: 21600962
    13. Nicastrin acts to stabilize gamma-secretase but is not required for substrate recognition PMID: 20130175
    14. Mature glycosylation and trafficking modulate its binding to presenilins PMID: 12032140
    15. a genetic modifier on mouse distal chromosome 1, coinciding with the locus containing Nicastrin, influences presenilin-mediated Notch S3-site cleavage and the resultant Notch phenotype without affecting presenilin-mediated APP gamma-site cleavage PMID: 12388777
    16. In the absence of presenilin 1 and 2, nicastrin remains entirely endoglycosidase H sensitive, is retained in the endoplasmic reticulum and is slowly degraded. PMID: 12584255
    17. effect of presenilin 1 and presenilin 2 on maturation of nicastrin in mammalian brain PMID: 12646573
    18. Nicastrin-null mouse embryos died by embryonic day 10.5 and exhibited several patterning defects, including abnormal somite segmentation, phenotypes that are reminiscent of embryos lacking Notch1 or both presenilins PMID: 12716934
    19. Nicastrin is essential for the gamma-secretase cleavage of APP and Notch in mammalian cells and that Nicastrin has both positive and negative functions in the regulation of gamma-secretase activity PMID: 12815056
    20. Presenilin-1 and nicastrin may participate in neuronal differentiation during neurogenesis in vitro PMID: 14511111
    21. presenilin 1 (PS1)-derived fragments, mature nicastrin, APH-1, and PEN-2, associate with cholesterol-rich detergent insoluble membrane (DIM) domains of non-neuronal cells and neurons PMID: 15322084
    22. Nct has a critical role in the stability and proper intracellular trafficking of other components of the PS1/ gamma-secretase complex but not in maintaining the association of PEN-2, APH-1, and full-length PS1 PMID: 15711015
    23. presenilin, nicastrin, APH-1, and PEN-2, are present and enriched on phagosome membranes from both murine macrophages and Drosophila S2 phagocytes PMID: 16103123
    24. Nicastrin deficiency drastically lowers neprilysin expression, membrane-bound activity and mRNA levels. PMID: 16606360
    25. Here, we have observed that NCT N-linked oligosaccharides mediated specific interactions with the secretory pathway lectins calnexin and ERGIC-53 PMID: 16938437
    26. Partial reduction (maintaining a level above 50% of normal) of brain nicastrin would likely not be efficacious in reducing functional PS-complexes and gamma-secretase activity as a therapeutic strategy for Alzheimer's disease. PMID: 17071095
    27. The results establish a novel mechanism linking the EGFR pathway to the tumor suppressor role of gamma-secretase. PMID: 17827153
    28. main role of the Glu(332) is in the maturation and assembly of gamma-secretase rather than in the recognition of the substrates PMID: 18502756
    29. S-palmitoylation plays a role in stability and raft localization of nicastrin and APH-1, but does not directly modulate gamma-secretase processing of APP and other substrates. PMID: 19028695
    30. Synoviolin-mediated ubiquitination is involved in the degradation of immature nicastrin, and probably regulates amyloid beta-protein generation. PMID: 19725872

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein. Cytoplasmic vesicle membrane; Single-pass type I membrane protein. Melanosome.
  • 蛋白家族：
    Nicastrin family
  • 数据库链接：

    KEGG: mmu:59287

    STRING: 10090.ENSMUSP00000003550

    UniGene: Mm.218203