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  4. Recombinant Mouse Cadherin-5 (Cdh5)

Recombinant Mouse Cadherin-5 (Cdh5)

  • 中文名称：
    Recombinant Mouse Cadherin-5(Cdh5)
  • 货号：
    CSB-CF005054MO
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdh5; Cadherin-5; Vascular endothelial cadherin; VE-cadherin; CD antigen CD144
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    46-784
  • 氨基酸序列
    DWIWNQMHIDEEKNESLPHYVGKIKSNVNRQNAKYVLQGEFAGKIFGVDANTGNVLAYERLDREKVSEYFLTALIVDKNTNKNLEQPSSFTVKVHDINDNWPVFSHQVFNASVPEMSAIGTSVIRVTAVDADDPTVAGHATVLYQIVKGNEYFSIDNSGLIFTKIKNLDREKQAEYKIVVETQDALGLRGESGTATVMIRLEDINDNFPVFTQSTYTFSVPEDIRVGKPLGFLTVVDPDEPQNRMTKYSIMQGEYRDTFTIETDPKRNEGIIKPTKSLDYEVIQQYTFYIEATDPTIRYEYLSSTSGKNKAMVTINVLDVDEPPVFQRHFYHFKLPENQKKPLIGTVVAKDPDKAQRSIGYSIRKTSDRGQFFRITKQGNIYNEKELDRETYAWYNLTVEANELDSRGNPVGKESIVQVYIEVLDENDNPPEFAQPYEPKVCENAAQGKLVVQISATDKDVVPVNPKFKFALKNEDSNFTLINNHDNTANITVKYGQFNREHAKFHYLPVLISDNGVPSLTGTSTLTVGVCKCNEQGEFTFCEEMAAQAGVSIQALVAIFLCILTITVITLLIILRRRIRKQAHAHSKSALEIHEQLVTYDEEGGGEMDTTSYDVSVLNSVRGGSTKPLRSTMDARPAVYTQVQKPPRLAPGLHGGPREMATMIDVKKEEADNDGGGPPYDTLHIYGYEGAESIAESLSSLSTNSSDSDIDYDFLNDWGPRFKMLAELYGSDPQEELII
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. This cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. It associates with alpha-catenin forming a link to the cytoskeleton. Acts in concert with KRIT1 and PALS1 to establish and maintain correct endothelial cell polarity and vascular lumen. These effects are mediated by recruitment and activation of the Par polarity complex and RAP1B. Required for activation of PRKCZ and for localization of phosphorylated PRKCZ, PARD3, TIAM1 and RAP1B to the cell junction.
  • 基因功能参考文献：
    1. endothelial VE-cadherin is involved in the reconstruction of the blood-brain barrier following ischemic stroke PMID: 29205257
    2. TM, especially TME45, maintains vascular integrity, at least in part, via Src signaling. PMID: 27643869
    3. These results suggest that SHP-2-via association with ICAM-1-mediates ICAM-1-induced Src activation and modulates VE-cadherin switching association with ICAM-1 or actin, thereby negatively regulating neutrophil adhesion to endothelial cells and enhancing their transendothelial migration. PMID: 28701303
    4. Galphas depletion blocks the S1PR1-activation induced VE-cadherin stabilization at junctions. PMID: 26674379
    5. Rab11a/Rab11 family-interacting protein 2-mediated VE-cadherin recycling is required for formation of adherens junctions and restoration of vascular endothelial barrier integrity. PMID: 26663395
    6. These findings together demonstrate the essential role of KDM4A and KDM4C in orchestrating mESC differentiation to endothelial cells through the activation of Flk1 and VE-cadherin promoters, respectively PMID: 26120059
    7. In the absence of Tie-2, VE-PTP inhibition destabilizes endothelial barrier integrity in agreement with the VE-cadherin-supportive effect of VE-PTP. PMID: 26642851
    8. identification of novel components of the adherens junction complex, and introduction of a novel molecular mechanism through which the VE-cadherin complex controls YAP transcriptional activity PMID: 26668327
    9. Endotoxin challenge initiates interrelated changes in microvessel Cx43, VE-cadherin, and microvessel permeability, with changes in Cx43 temporally leading the other responses. PMID: 26163513
    10. Mutating Y731 in the cytoplasmic tail of VE-cadherin, known to selectively affect leukocyte diapedesis, but not the induction of vascular permeability, attenuates bleeding. PMID: 26169941
    11. mRNA of HIF-2alpha and Ets-1 were significantly increased by HIF-3alpha ablation. Both factors activate the VE-cadherin gene, the transcriptional repression of these factors by HIF-3alpha is important for silencing the irrelevant expression of the VE-cadherin PMID: 25626335
    12. iPS cell-derived Flk1(+)VE-cadherin(+) cells expressing the Er71 are as angiogenic as mES cell-derived cells and incorporate into CD31(+) neovessels. PMID: 24386480
    13. VE-cadherin tyrosine phosphorylation at Y685 is a physiological and hormonally regulated process in female reproductive organs. PMID: 24858855
    14. Conclude that the site Y685 in VE-cadherin is involved in the physiological regulation of capillary permeability in mouse ovary/uterus. PMID: 24858856
    15. Findings support the importance of adhesion molecules (VE-cadherin and CD31), survivin, and Ajuba in modulating the Hippo pathway, which regulates, in part, proliferation and survival in hemangioendotheliomas. PMID: 25266662
    16. role of eNOS and VE-cadherin in angiopoietin-1 regulation of microvascular reactivity and protection of the microcirculation during acute endothelial dysfunction PMID: 24407281
    17. Tyr685 and Tyr731 of VE-cadherin distinctly and selectively regulate the induction of vascular permeability or leukocyte extravasation PMID: 24487320
    18. We suggest that lymphocyte binding to vascular cell adhesion molecule 1 triggers a signaling process that enables a VE-PTP substrate to dissociate VE-PTP from VE-cadherin, thereby facilitating efficient transmigration. PMID: 23908467
    19. We found that activation of Notch1 in the endothelial compartment in VE-cadherin expressing cells resulted in the absence of intra-aortic clusters and defects in fetal liver hematopoiesis. PMID: 22851140
    20. Phosphorylation of vascular endothelial cadherin contributes to a dynamic state of adherens junctions. PMID: 23169049
    21. This correlates with inhibition of vascular endothelial growth factor-A (VEGF-A)-induced signaling and stabilization of vascular endothelial (VE)-cadherin localization at endothelial junctions. PMID: 22975327
    22. VN plays a previously unrecognized role in regulating endothelial permeability via conformational- and integrin-dependent effects on VE-cadherin trafficking. PMID: 22606350
    23. Data suggest that VE-cadherin controls vascular permeability and limits fibrogenesis after unilateral ureteral obstruction (UUO). PMID: 22126970
    24. Angiogenesis is stimulated by mini-TyrRS and inhibited by mini-TrpRS by VE-cadherin signaling events. PMID: 21442253
    25. The cadherin Cdh5 (Vecad) maintains high expression variability only during fetal development, while the integrin subunit Itga5 (alpha5) increases its variability after 14.5 dpc. PMID: 22276210
    26. These results establish the junctional route as the main pathway for extravasating leukocytes in several tissues, for which the plasticity of the cadherin-5-alpha-catenin complex is central for the leukocyte diapedesis mechanism. PMID: 21857650
    27. CRSBP-1 ligands induce disruption of VE-cadherin-mediated intercellular adhesion and opening of intercellular junctions in lymphatic endothelial cell. PMID: 21444752
    28. By demonstrating that Wt1(-KTS) protein trans-activates an enhancer element in the first intron we identified CDH5 as a novel target gene of Wt1. PMID: 20811903
    29. key role of KLF4 in the regulation of VE-cadherin expression at the level of the adheren junctions and in the acquisition of VE-cadherin-mediated endothelial barrier function PMID: 20724706
    30. The VE-Cad complex with pulmonary adenoma resistance 3 (Par3) protein highlights how the unique molecular architecture of par3-PDZ3 can accommodate both canonical and distal interaction modes. PMID: 20047332
    31. VEC may influence the constitutive organization of the actin cytoskeleton. PMID: 11950930
    32. Data show that vascular endothelial protein tyrosine phosphatase (VE-PTP) is a transmembrane binding partner of VE-cadherin that reduces the tyrosine phosphorylation of VE-cadherin and cell layer permeability. PMID: 12234928
    33. Data suggest that ADAM15, whose expression may be driven by VE-cadherin, may be a component of adherens junctions and play a role in endothelial functions mediated by these cell contacts. PMID: 12243749
    34. VE-cadherin-beta-catenin complex participates in contact inhibition of VEGF signaling PMID: 12771128
    35. Data show that sustained activation of MAPK/ERKs results in disruption of VE-cadherin-mediated cell-cell adhesion, down-regulation of PECAM-1 expression, and enhanced cell migration in microvascular endothelial cells. PMID: 12938162
    36. Cdh5 expression and clustering maintain low levels of survivin in endothelial cells. PMID: 15215174
    37. de novo blood vessel formation up to when a vascular epithelium forms is not dependent on VE-cadherin and VE-cadherin, whose expression is up-regulated following vascular epithelialization, is required to prevent the disassembly of nascent blood vessels. PMID: 15604224
    38. transcription is controlled by the hypothalamo-pituitary axis PMID: 15691883
    39. analysis of endothelial cell-specific gene expression of the 5' flanking region and the 5' half of the first intron of the VE-cadherin gene PMID: 15746076
    40. virtually all HSC activity from embryonic day 13.5 (E13.5) fetal liver was CD144+. CD144 expression declined on E16.5 fetal liver HSCs and was absent from adult bone marrow HSCs PMID: 15831702
    41. MIP-1beta is overexpressed, and VE-cadherin is underexpressed in heart transplant allografts compared with isografts PMID: 15897346
    42. downregulation of VE-cadherin in endothelial tumors may have important consequences for tumor growth and bleeding complications PMID: 15968386
    43. PECAM-1, vascular endothelial cell cadherin, and VEGFR2 comprise a mechanosensory complex: together these receptors are sufficient to confer responsiveness to fluid shear stress in heterologous cells PMID: 16163360
    44. A new ischemia-induced neovascularization mechanism involving VE-cadherin, re-expressed VE-cadherin mediating cell adhesion. PMID: 16543497
    45. HIF-2alpha cooperates with the Ets-1 transcription factor for activation of the VE-cadherin promoter and that this synergy is dependent on the binding of Ets-1 to DNA. PMID: 17563748
    46. Vascular sprout formation entails tissue deformations and Cdh5-dependent cell-autonomous motility. PMID: 18062955
    47. VE-cadherin has a role in modulating c-Src activation in VEGF signaling PMID: 18180305
    48. Measured changes in VE-cadherin after cardiac arrest in mouse lung in relation to warm ischemia time and lung injury. PMID: 18281348
    49. VE-cadherin promotes breast cancer progression via transforming growth factor beta signaling PMID: 18316602
    50. These data indicate that VE-cadherin is a positive and endothelial cell-specific regulator of TGF-beta signalling. PMID: 18337748

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  • 亚细胞定位：
    Cell junction. Cell membrane; Single-pass type I membrane protein.
  • 组织特异性：
    Expressed in postnatal endothelial cells of the retinal vascular plexus (at protein level).
  • 数据库链接：

    KEGG: mmu:12562

    STRING: 10090.ENSMUSP00000034339

    UniGene: Mm.21767