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Recombinant Mouse Apolipoprotein C-I (Apoc1)

In Stock
  • 货号：
    CSB-CF001930MO
  • 规格：
    ￥5028
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 90% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    Apoc1; Apolipoprotein C-I; Apo-CI; ApoC-I; Apolipoprotein C1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    12.5 kDa
  • 表达区域：
    27-88aa
  • 氨基酸序列
    APDLSGTLESIPDKLKEFGNTLEDKARAAIEHIKQKEILTKTRAWFSEAFGKVKEKLKTTFS
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

我购买了CSB-CF001930MO，批次YA05476b1g5，请问能否确认浓度是说明书上显示的0.4mg/ml？我能用DMEM f12稀释到我想要的浓度吗？其最佳处理方法是什么？

A:
CSB-CF001930MO 批次YA05476b1g5， bardford测得浓度0.4mg/ml， 对应缓冲：0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 建议您用与蛋白相同或相近缓冲进行稀释，建议稀释后浓度不低于0.1mg/ml，有利于蛋白的稳定和保存；

靶点详情

  • 功能：
    Inhibitor of lipoprotein binding to the low density lipoprotein (LDL) receptor, LDL receptor-related protein, and very low density lipoprotein (VLDL) receptor. Associates with high density lipoproteins (HDL) and the triacylglycerol-rich lipoproteins in the plasma and makes up about 10% of the protein of the VLDL and 2% of that of HDL. Appears to interfere directly with fatty acid uptake and is also the major plasma inhibitor of cholesteryl ester transfer protein (CETP). Modulates the interaction of APOE with beta-migrating VLDL and inhibits binding of beta-VLDL to the LDL receptor-related protein. Binds free fatty acids and reduces their intracellular esterification.
  • 基因功能参考文献：
    1. APOC1 expression induces glomerulosclerosis, potentially by increasing the cytokine response in macrophages. PMID: 27976371
    2. Apolipoprotein C-I was significantly increased in obese mice plasma. PMID: 22404376
    3. The absence of ApoC-I results in impaired memory functions, which is, together with previous data, suggestive of an important, bell-shaped gene-dose dependent role for ApoC-I in appropriate brain functioning PMID: 21157034
    4. Data show that the stimulating effect of apoCI on the lipopolysaccharide (LPS)response resembles that of LPS-binding protein (LBP) and depends on CD14/ Toll-like receptor 4 signaling. PMID: 20335569
    5. regulated expression of gene cluster in macrophages PMID: 12032151
    6. present observations provide direct support for a potent specific inhibition of CETP by plasma apoCI in vivo PMID: 12070157
    7. The apoC-I content of lipoprotein remnants may serve as an early marker of coronary artery disease risk. PMID: 12231568
    8. TR4 can also regulate apolipoprotein E, C-I, and C-II gene expression via the TR4 response element within the hepatic control region PMID: 12954636
    9. apoC-I is a potent inhibitor of LPL-mediated triglyceride lipolysis PMID: 15576844
    10. irrespective of receptor-mediated remnant clearance by the liver, liver-specific expression of recombinant human apoCI causes hypertriglyceridemia in the absence of the VLDLr and apoCIII in mice PMID: 16478678
    11. Endogenous apoC-I increases hyperlipidemia in apoE-knockout mice by stimulating VLDL production and inhibiting LPL. PMID: 16537968
    12. Knockout mice show significant increases in the hepatic content of cholesteryl esters and triglycerides and in biliary cholesterol concentration. PMID: 17053273
    13. Systemic apoCI increases atherosclerosis, probably by inducing hyperlipidemia, in the absence of ApoE PMID: 17320883
    14. Data suggest that apoC-I binds free fatty acids (FFA), thereby reducing the blood FFA for uptake by cells, and this mechanism can serve as an additional mechanism for the resistance to obesity and the cutaneous abnormalities of APOC1(+/+) mice. PMID: 17339654
    15. apoCI is crucially involved in lipopolysaccharide(LPS)-induced atherosclerosis in apoe-/- mice, which mainly relates to an increased inflammatory response toward LPS PMID: 17967778
    16. apoCI is a novel inhibitor of the scavenger receptor BI -mediated uptake of HDL cholesterol by hepatocytes; apoCI is a determinant for the plasma levels and size of HDL in vivo. PMID: 18992221

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Apolipoprotein C1 family
  • 组织特异性：
    Adult and fetal liver.
  • 数据库链接：

    KEGG: mmu:11812

    STRING: 10090.ENSMUSP00000045571

    UniGene: Mm.182440