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  4. Recombinant Human Cannabinoid receptor 2 (CNR2)

Recombinant Human Cannabinoid receptor 2 (CNR2)

  • 货号：
    CSB-CF005679HU
  • 规格：
    ￥9720
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
  • Uniprot No.：
  • 别名：
    CNR2; CB2A; CB2B; Cannabinoid receptor 2; CB-2; CB2; hCB2; CX5
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Full Length
  • 来源：
    in vitro E.coli expression system
  • 分子量：
    42.5 kDa
  • 表达区域：
    1-360aa
  • 氨基酸序列
    MEECWVTEIANGSKDGLDSNPMKDYMILSGPQKTAVAVLCTLLGLLSALENVAVLYLILSSHQLRRKPSYLFIGSLAGADFLASVVFACSFVNFHVFHGVDSKAVFLLKIGSVTMTFTASVGSLLLTAIDRYLCLRYPPSYKALLTRGRALVTLGIMWVLSALVSYLPLMGWTCCPRPCSELFPLIPNDYLLSWLLFIAFLFSGIIYTYGHVLWKAHQHVASLSGHQDRQVPGMARMRLDVRLAKTLGLVLAVLLICWFPVLALMAHSLATTLSDQVKKAFAFCSMLCLINSMVNPVIYALRSGEIRSSAHHCLAHWKKCVRGLGSEAKEEAPRSSVTETEADGKITPWPDSRDLDLSDC
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
  • 基因功能参考文献：
    1. Among 1027 obese patients, genotypes GG, GA, and AA were found in 339 (33.0%), 467 (45.5%), and 221 (21.5%) respectively. Body mass index, weight, fat mass, waist circumference, insulin, HOMA-IR, and triglyceride and leptin levels were higher in A-allele carriers as compared to non A-allele carriers. No differences were seen in these parameters between the GA and AA genotypes. PMID: 28895540
    2. in immune cells, activation of both CB2R variants 63Q and 63R causes phosphorylation of ERK; however, the signal intensity caused by 63R activation is relatively weaker than that caused by 63Q activation PMID: 29694791
    3. The cannabinoid receptor type 2 (CB2) Q63R variation is associated with increased risk of hospitalization in children with acute respiratory tract infection (ARTI). Children carrying the QQ genotype are more prone to developing severe ARTI. The associated risk of developing severe ARTI following RSV infection increases in children carrying the Q allele PMID: 28992427
    4. Urinary bladder cancer cell growth and motility implicate cannabinoid 2 receptor-mediated modifications of sphingolipids metabolism. PMID: 28191815
    5. Study shows that this endogenous fatty acid ethanolamide affects cannabinoid signaling by upregulating cannabinoid receptor 2 expression in mononuclear phagocytic cells. PMID: 28336953
    6. This study found enhanced CNR2 mRNA levels in all Low Back Pain participants. PMID: 28481838
    7. Carriers of the minor allele of rs3123554 variant of CB2R gene loose less body weight during two different hypocaloric diets. The improvement of metabolic parameters was better in no A allele carriers than A allele carriers. PMID: 29518488
    8. CB2 activation with sub-micromolar doses of agonists, which could be more similar to endogenous levels of cannabinoids, promote colon cancer progression in a process that involves AKT and GSK3beta. PMID: 27634891
    9. stimulation of MAPK contributes to the peripheral analgesic effect of CB2 receptor agonists. PMID: 26108183
    10. Describe PM226, a chromenoisoxazole, as a selective CB2 receptor agonist with neuroprotective properties. PMID: 27013280
    11. Our data indicate that CB2 may directly contribute to the pathogenesis of eosinophil-driven diseases. Moreover, we provide new insights into the molecular mechanisms underlying the CB2 -mediated priming of eosinophils. PMID: 26850094
    12. analysis of the interplay between the CB2-RR variant and liver necroinflammation in chronic hepatitis patients with HIV/HCV coinfection PMID: 28759568
    13. Patients with schizophrenia showed increased CB2R expression in cells of the innate immune system and simpler correlation network between cytokines and CBRs expression when compared with controls. PMID: 28011441
    14. Cannabinoid Receptor 2 mutation is associated with obesity. PMID: 27294325
    15. The type 2 cannabinoid (Cnr2) receptor is implicated in cancer, bone metabolism and pain perception. Emerging data have uncovered the role of Cnr2 in the regulation of tumour-bone cell interactions and suggest that agents that target Cnr2 in the skeleton have potential efficacy in the reduction of skeletal complications associated with cancer. [review] PMID: 28274851
    16. The prevalence of chronic HCV infected patients with the CB2-63 RR variant was significantly higher in the immune-mediated disorder (IMD) than in the non-IMD group. The data suggest a significant, previously unknown, independent association between the CB2-63 RR variant and IMDs in anti-HCV-positive patients. PMID: 27476469
    17. CNR2 gene has an important role in the etiology of osteoporosis and suggest that it may be a genetic risk factor for BMD and osteoporosis in Han Chinese postmenopausal women. PMID: 26055357
    18. A histological activity index (HAI) > 8 (Ishak scoring) was more frequent in patients with CB2-63 RR than in those with CB2-63 QR or QQ (37% vs. 16.7%, p < 0.05). PMID: 25749560
    19. Study demonstrates the up-regulation of CB2 receptors in glial elements in postmortem tissues of Parkinson's disease patients and an inflammatory mouse model PMID: 25863279
    20. Association between the CB2 Q63R functional variant and the age at menarche in obese girls. PMID: 26447698
    21. CB2 receptor activation can attenuate HAND severity by inhibiting HIV replication, blunting HIV-triggered neuroinflammation, preventing HIV-induced damage to Blood-brain-barrier integrity, and preventing HIV-associated synapse loss. PMID: 25015040
    22. Data show that HU308 and JWH133 enhanced human and mouse breast cancer cell-induced osteoclastogenesis and exacerbated osteolysis. PMID: 26195631
    23. Targeting CB2 may represent an attractive approach to treat cancers of immune origin. PMID: 25640641
    24. results suggest that rs2501431, rs3003336, rs2229579, and rs4237 polymorphisms in CNR2 genes may be genetic factors affecting bone mineral density in Korean postmenopausal women PMID: 25268406
    25. no significant differences between preeclamptic and normal placenta in terms of CB2 and FAAH expressions and immunoreactivity PMID: 25444073
    26. up-regulated expression of hCB2R could induce cell apoptosis by enhancing the expressions of Bax, Bad and suppressing the expression of Bcl-2 in Caski cells PMID: 26062417
    27. data from this study suggested that activation of CB2 receptor plays important role in osteogenic differentiation of BM-MSCs. Lack of CB2 receptor may be related to osteoporosis. PMID: 25685815
    28. Cannabinoid receptor type 2 contributes to susceptibility to oligo/polyarticular juvenile idiopathic arthritis and to the severity of its clinical course. PMID: 25974389
    29. These pilot findings suggest that CB2 Q63R polymorphism does not play a major role in genetic susceptibility to inflammatory bowel disease or in its disease phenotypes among Turkish subjects PMID: 25599774
    30. findings reveal an unprecedented role of CB2 as a pivotal regulator of HER2 pro-oncogenic signaling in breast cancer, and they suggest that CB2 may be a biomarker with prognostic value in these tumors. PMID: 25855725
    31. The CB2-63 QQ variant in HCV patients was independently associated with the persistently normal alanine aminotransferase status. PMID: 24940753
    32. The present study shows CB2 is expressed in human post-mortem striatum in Huntington's disease PMID: 24978314
    33. Amino acids Valine113 and Leucine192 play important roles in ligand binding and downstream signaling transduction of the CB2 receptor. PMID: 25148941
    34. CB2R and GPR55 form heteromers in cancer cells, these structures possess unique signaling properties, and modulation of these heteromers can modify the antitumoral activity of cannabinoids in vivo PMID: 24942731
    35. Increased CB2 mRNA and anandamide in human blood after cessation of cannabis abuse PMID: 24788457
    36. Activation of CB2 receptors reduces adhesion and transmigration of melanoma cells through the cerebral endothelium. PMID: 24815068
    37. association of the minor allele of SNP rs3123554 within the CNR2 gene with lower BMI and body fat; significant association of the SNP with BMI was limited to females PMID: 23839870
    38. CB2R activation drives an altered phenotype of foam cells, by negatively regulating lipid influx and/or by modulating cytokine profile PMID: 24529123
    39. The mRNA level for cannabinoid receptor type 2 (CB2) was significantly increased in peripheral blood mononuclear cells from autistic children as compared to healthy subjects PMID: 23585028
    40. applied a comprehensive G protein-coupled receptor-Galphai protein chemical cross-linking strategy to map the cannabinoid receptor subtype 2 (CB2)-Galphai interface and then used molecular dynamics simulations to explore the dynamics of complex formation PMID: 24855641
    41. Data suggest that ligand binding to CNR2 (either recombinant or constitutive) is important to agonist action (and presumably antinociceptive action) of ligands (despite low signal obtained in native tissues/spleen). PMID: 23711022
    42. The functional role of intracellular CB2 receptors has been defined and linked to calcium signaling. PMID: 25033246
    43. The global fold of human cannabinoid type 2 (CB2 ) receptor in the agonist-bound active state in lipid bilayers was studied. PMID: 23999926
    44. Antagonism of cannabinoid receptor 2 pathway suppresses IL-6-induced immunoglobulin IgM secretion. PMID: 24913620
    45. cultures of hMSCs express all of the components of the endocannabinoid system and suggest a potential role for the cannabinoid CB2 receptor as a mediator of MSC anti-inflammatory properties, as well as for their survival pathways. PMID: 24312195
    46. The CB2-63 QQ variant of CNR2 is associated with more severe inflammation and hepatocellular necrosis in patients with HCV infection. PMID: 23707465
    47. CB2 receptor expression in proximal tubule cells is modulated by internalization of albumin. PMID: 24280624
    48. Report synthesis of carbazole derived CB(2) ligands/agonists with increased polarity. PMID: 24243315
    49. In rheumatoid arthritis patients' synovial fibroblasts, proinflammatory mediators up-regulate the expression of cannabinoid receptor 2, which negatively regulates the production of proinflammatory cytokines and matrix metalloproteinases. PMID: 24440992
    50. selective CB2 activation in leukocytes decreases key steps in monocyte-blood-brain barrier engagement. PMID: 24055259

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Perikaryon.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Preferentially expressed in cells of the immune system with higher expression in B-cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spl
  • 数据库链接：

    HGNC: 2160

    OMIM: 605051

    KEGG: hsa:1269

    STRING: 9606.ENSP00000363596

    UniGene: Hs.73037