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  4. Recombinant Human 5-hydroxytryptamine receptor 4 (HTR4)

Recombinant Human 5-hydroxytryptamine receptor 4 (HTR4)

  • 货号：
    CSB-CF622677HU
  • 规格：
  • 来源：
    in vitro E.coli expression system
  • 其他：

产品详情

  • 基因名：
  • Uniprot No.：
  • 别名：
    HTR4; 5-hydroxytryptamine receptor 4; 5-HT-4; 5-HT4; Serotonin receptor 4
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-388
  • 氨基酸序列
    MDKLDANVSSEEGFGSVEKVVLLTFLSTVILMAILGNLLVMVAVCWDRQLRKIKTNYFIV SLAFADLLVSVLVMPFGAIELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLCCISLDRYY AICCQPLVYRNKMTPLRIALMLGGCWVIPTFISFLPIMQGWNNIGIIDLIEKRKFNQNSN STYCVFMVNKPYAITCSVVAFYIPFLLMVLAYYRIYVTAKEHAHQIQMLQRAGASSESRP QSADQHSTHRMRTETKAAKTLCIIMGCFCLCWAPFFVTNIVDPFIDYTVPGQVWTAFLWL GYINSGLNPFLYAFLNKSFRRAFLIILCCDDERYRRPSILGQTVPCSTTTINGSTHVLRD AVECGGQWESQCHPPATSPLVAAQPSDT
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
  • 基因功能参考文献：
    1. found that testosterone, but not estradiol, correlated negatively with global 5-HT4R levels suggesting that men with high levels of testosterone have higher cerebral serotonergic tonus. PMID: 28426945
    2. l-Lysine significantly reduced the surge of plasma aldosterone induced by metoclopramide indicating that l-Lysine is able to efficiently antagonize the adrenal 5-HT4 receptors in vivo. PMID: 28103616
    3. Acute administration of 5-hydroxytryptamine4 (5-HT4) receptor agonist, mosapride or esophageal infusion of the transient receptor potential vanilloid receptor-1 (TRPV1) agonist capsaicin promotes secondary peristalsis.Esophageal infusion with capsaicin-containing red pepper sauce suspension does create greater mechanosensitivity as measured by secondary peristalsis than 5-HT4 receptor agonist mosapride PMID: 27438088
    4. Findings replicate our previous observation of a negative association between 5-HT 4R binding and memory performance in an independent cohort and provide novel evidence linking 5-HT 4R binding, as a biomarker for synaptic 5-HT levels, to the mnestic processing of positive and neutral word stimuli in healthy humans PMID: 28413715
    5. Results of this study indicated that HTR4 signaling upregulated ERbeta expression in hormone-naive prostate cancer and could impact on biological processes in hormone-naive prostate cancer. PMID: 28195088
    6. Data suggest that the 5-HT4 receptor is involved in the neurobiological mechanism underlying familial risk for depression, and that lower striatal 5-HT4 receptor binding is associated with increased risk for developing major depression disorder PMID: 25522384
    7. PDE3A1 and PDE4D3 are integrated into complexes that contain the 5-HT4(b) receptor and may thereby regulate 5-HT4(b) receptor-mediated signaling. PMID: 25101859
    8. The findings of altered lung function and increased AHR in Htr4-null mice support a causal relationship between genetic variation in HTR4 and pulmonary function identified in human genome-wide association studies. PMID: 25342126
    9. This study demonistrated that 5-hydroxytryptamine (serotonin) receptor 4 regulate memory. PMID: 25622143
    10. ADAM19 rs1422795 and HTR4 rs11168048 are associated with pulmonary function. PMID: 24951661
    11. This is the first transgenic model to study human 5-HTreceptor in the atrium ex vivo or in vivo. PMID: 23307014
    12. Stimulation of central 5-HT4 receptors is potentially disease-modifying in a transgenic mouse model of Alzheimer's disease. PMID: 23474291
    13. Taken together, these data suggest a role for HTR4 in lung development, which may at least in part explain the genetic association with lung function. PMID: 23890215
    14. Our results suggest that HTR4 polymorphisms may not play a major role in the susceptibility for suicidal behavior in subjects with schizophrenia. PMID: 22842674
    15. These results suggest a prominent role of 5-HT(4)R in promoting angiogenesis. PMID: 22903372
    16. Results suggest an important role for the CHRNA5/3 region as a genetic risk factor for airflow obstruction independent of smoking and implicate the HTR4 gene in the etiology of airflow obstruction. PMID: 22837378
    17. Our findings are consistent with a model wherein the 5-HTTLPR S allele is associated with relatively increased serotonin levels. PMID: 22584237
    18. These findings suggest that the 5-HT(4)R is critically involved in reward circuits that regulate people's food intake PMID: 22709820
    19. Mucosal 5-HT(4) receptor activation can mediate the prokinetic and antinociceptive actions of 5-HT(4)R agonists. PMID: 22226658
    20. The occurrence of heterogeneous post-translational modifications (PTMs) on transgenic proteins, as well as the complications that non-native PTMs can cause, highlight the characterization of both endogenous and heterologous protein targets. PMID: 22145929
    21. 5-HT4 receptor binding was a positively correlated to amyloid beta (A4) burden and negatively to MMSE score of the Alzheimer disease patients PMID: 21673407
    22. HTR4 polymorphism associated with COPD risk and lung function decline PMID: 21965014
    23. SNP and haplotypes of the HTR4 gene were associated with the asthma phenotype and genetic variation of HTR4 may affect susceptibility to the development of asthma. PMID: 21382128
    24. The relatively stable HT4 5- receptor binding with aging contrasts others in subtypes of receptors, which generally decrease with aging. PMID: 21364600
    25. This study demonistrated that 5-HT4 receptor is present in the human thalamus prenatally. PMID: 20538346
    26. No association was found in the family sample betweewn HTR4 and schizophrenia. PMID: 19892407
    27. Data show expression of TNS1, GSTCD, AGER, HTR4 and THSD4 in lung tissue and indicate potential targets for interventions to alleviate respiratory disease. PMID: 20010834
    28. Computational model of the complex between GR113808 and the 5-HT4 receptor guided by site-directed mutagenesis and the crystal structure of rhodopsin. PMID: 11989623
    29. The polymorphisms associated with mood disorder were located within the region that encodes the divergent C-terminal tails of the 5-HT(4) receptor. PMID: 12399948
    30. report the activation of the extracellular signal-regulated kinases (ERKs) 1 and 2 (p44 and p42 MAP kinase) through the human serotonin receptors 5-HT(4(b)) and 5-HT(7(a)) PMID: 12446729
    31. results show an overexpression of the 5-HT4 receptor in cisapride-responsive ACTH-independent bilateral macronodular adrenal hyperplasia PMID: 12519861
    32. A highly significant association between schizophrenia and haplotype A-T (OR = 0.13 [0.03-0.58]) was detected. PMID: 12898568
    33. Our results suggest a complex regulation of the h5-HT4 receptor gene expression involving distinct promoters and non-coding exons. PMID: 15575821
    34. secretion of the non-amyloidogenic form of amyloid precursor protein, sAPPalpha induced by the 5-HT4(e) receptor isoform was not due to a general boost of the constitutive secretory pathway but rather to its specific effect on alpha-secretase activity PMID: 15710402
    35. inhibition of Na+/H+ exchange activity by serotonin is mediated by 5-HT4 receptors in Caco-2 cells PMID: 15825078
    36. coexpression of h5-HT4R and beta2-adrenergic receptor (beta2AR) led to their heterodimerization PMID: 15896782
    37. the uncoupling and endocytosis of 5-HT4R require different GRK2 concentrations and involve distinct molecular events PMID: 15919661
    38. development of an adenovirus expression system to examine the properties of two human 5-HT4 receptor splice variants, h5-HT4(b) and h5-HT4(d), expressed in adult cardiomyocytes devoid of native 5-HT4 receptors PMID: 15950987
    39. These findings provide the first evidence of differential internalization between the two splice variants, 5-HT(4a) and 5-HT(4b) receptors. PMID: 16209130
    40. We show overexpression & different splicing of 5-HT4 receptor in aldosterone-producing adenoma tissues in comparison with normal adrenocortical tissue.Isoforms (a) & (b) were not expressed in any APA but were present in majority of normal adrenal cortex. PMID: 16322401
    41. These results suggest that the HTR4 gene may play a role in the genetic predisposition to ADHD. PMID: 16563621
    42. Data show that 5-HT(4) receptor stimulation in primary neurons produced a potent but transient activation of the ERK pathway that is dependent on Src tyrosine kinase but totally independent of beta-arrestin. PMID: 17377064
    43. We show that disulfide bridges between Cys112 and Cys145 located within transmembrane domain 3 and transmembrane domain 4, respectively, are of critical importance for 5-HT(4)R dimer formation. PMID: 17379184
    44. Our results indicate that h5-HT(4(b)) is the dominant cardiac isoform of human 5-HT(4) receptors and its expression is increased in CAF. PMID: 17418812
    45. Functional activiy of 5-HT4 receptors was studied in children with congenital heart disease. PMID: 17603679
    46. In this review, the pathways regulating either beta- or gamma-secretase may be differentially controlled by 5-HT4 receptor isoforms. PMID: 18322379
    47. In failing human ventricle, 5-HT(4) receptor-mediated positive inotropic response was regulated by PDEs in a manner similar to that in postinfarction rat hearts;5-HT, PDE3 and PDE4 may have pathophysiological functions in heart failure. PMID: 18846035
    48. 5-HT4 expression is lower in heart ventricle vs. atrium. Isoform expression in atrium and ventricle is similar. There is a parallel increase of cAMP and in the phosphorylation state of regulatory proteins following stimulation with 5-HT in the atrium. PMID: 19002436
    49. The human 5HT4 receptor, when placed under the influence of the mouse opsin promoter and an opsin rod outer segment (ROS) targeting sequence, localized to ROS of transgenic mouse retina. PMID: 19053287
    50. This paper presents an analysis of the SH3TC2 promoter after identifying a read-through transcript of the SH3TC2 and HTR4 loci. Available data suggests HTR4 is a separate locus with its own promoter, and not the product of a bi-cistronic transcript. PMID: 11716477

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein. Endosome.
  • 蛋白家族：
    G-protein coupled receptor 1 family
  • 组织特异性：
    Isoform 5-HT4(A) is expressed in ileum, brain, and atrium, but not in the ventricle.
  • 数据库链接：

    HGNC: 5299

    OMIM: 602164

    KEGG: hsa:3360

    UniGene: Hs.483773