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  4. V5-Tag Monoclonal Antibody

V5-Tag Monoclonal Antibody

  • 货号：
    CSB-MA000161M1m
  • 规格：
    ￥400
  • 图片：
    • Western Blot
      Positive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, 5, 6 at 50ng
      All lanes: V5 antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 31.9, 20.4, 55, 24.2, 53.4, 30 KDa
      Observed band size: 32, 21, 55, 28, 58, 33 KDa
      Exposure time: 5min
    • Western Blot
      Positive WB detected in: 50ng V5-tagged fusion protein
      V5 Tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 32 KDa
      Observed band size: 32 KDa
      Exposure time: 5min
    • Western Blot
      Positive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:2000
      Lane 1: Recombinant V5-tagged fusion protein at 25ng
      Lane 2: Recombinant V5-tagged fusion protein at 12.5ng
      Lane 3: Recombinant V5-tagged fusion protein at 6.25ng
      Lane 4: Recombinant V5-tagged fusion protein at 3.125ng
      Lane 5: Recombinant V5-tagged fusion protein at 1.56ng
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 32 kd
      Observed band size: 32 kd
      Exposure time: 5min
    • Immunofluorescence staining of transfected HEK293F cells with CSB-MA000161M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).
    • Immunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate
      Lane 1: Mouse control IgG2a instead of CSB-MA000161M1m in Transfected HEK293 cells whole cell lysate
      Lane 2: CSB-MA000161M1m (2.5µl) + Transfected HEK293F cells whole cell lysate (500µg)
      Lane 3: Transfected HEK293F cells whole cell lysate (20µg)
      For western blotting, the blot was detected with CSB-MA000161M1m at 1:3000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000
  • 其他：

产品详情

  • 产品描述：

    Hybridoma technology was used in the production of this V5-Tag monoclonal antibody. The hybridoma was formed from the fusion of B cells and myeloma cells. The B cells were isolated from the spleen of the mouse immunized with the GKPIPNPLLGLDST synthetic peptide conjugate to KLH. Because of the two types of cells, the hybridoma could ensure the continuous production of the V5-Tag monoclonal antibody. This V5-Tag monoclonal antibody is subjected to protein A purification and gets a purity of up to 95%. It has been validated in ELISA, WB, IF, and IP.

    The V5 tag is a polypeptide composed of amino acid residues 95 to 108 isolated from the P protein and V protein of the simian virus 5 (SV5). The amino acid sequence: GKPIPNPLLGLDST. Because its small number of amino acids will not affect the properties of the target protein and it has high affinity with specific antibodies, the V5 tag is commonly added to the N- or C-terminus of the target protein to purify, detect, and trace the target protein.

  • 基因名：
    V5-Tag
  • 宿主：
    Mouse
  • 反应种属：
    All
  • 免疫原：
    GKPIPNPLLGLDST synthetic peptide conjugate to KLH
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2a
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    9E8B7
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:5000-1:1280000
    IF 1:100-1:200
    IP 2µl-5µl
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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