Your Good Partner in Biology Research

  1. Home
  2. 抗体
  3. 标签内参抗体
  4. GFP Monoclonal Antibody

GFP Monoclonal Antibody

  • 货号：
    CSB-MA000051M1m
  • 规格：
    ￥400
  • 图片：

    • Western Blot
      Positive WB detected in: 50ng recombinant protein
      All lanes: GFP antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 32 KDa
      Observed band size: 32 KDa
      Exposure time:5min

    • Western Blot
      Positive WB detected in: Recombinant protein at 25ng, 12.5ng, 6.25ng, 3.125ng, 1.56ng, 0.78ng, 0.39ng, 0.195ng
      All lanes: GFP antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 32 KDa
      Observed band size: 32 KDa
      Exposure time:5min

    • Western Blot
      Positive WB detected in: 1-4 lanes:Recombinant proteins with GFP tag for 50ng; 5 lane: 293F whole cell lysate transfected with GFP for 5μg
      All lanes GFP antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size:1,2,3,4 and 5 is 32,32,50,32,32 KDa respectively
      Observed band size: 32,32,50,32,32 KDa
      Exposure time:1min

    • Immunofluorescence staining of 293F cells transfected with GFP with CSB-MA000051M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).

    • Two-color flow cytometric analysis showing 293F cells untransfected (Left) or transfected with GFP (Right) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C.

    • Overlay histogram showing 293F cells untransfected (green line) or transfected with GFP (red line) stained with CSB-MA000051M1m at 1:200. The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C.

    • Immunoprecipitating GFP in 293F whole cell lysate transfected with GFP
      Lane 1: Mouse control IgG2b instead of CSB-MA000051M1m in 293F whole cell lysate transfected with GFP
      Lane 2: CSB-MA000051M1m (4µg) + 293F whole cell lysate transfected with GFP (500µg)
      Lane 3: 293F whole cell lysate transfected with GFP (5µg)
      For western blotting, the blot was detected with CSB-MA000051M1m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000

  • 其他：

产品详情

  • 产品描述：

    CUSABIO immunized the mouse with the recombinant GFP protein to get the splenocytes that secret the GFP antibody. The GFP-producing splenocytes were subsequently fused with myeloma cells to form hybridomas, which were inoculated into the abdominal cavity of mice. The mouse ascites was collected and purified to get the monoclonal anti-GFP antibody. Following purification through protein A, the purity of the monoclonal GFP antibody is more than 95%. It is matched with the mouse IgG2bisotype. And it targets the GFP tag in ELISA, WB, IF, IP, and FC applications and reacts with the GFP protein from all species.

    Green fluorescent protein (GFP) is a protein that exhibits bright green fluorescence when exposed to light in the blue to ultraviolet range. For this reason, GFP is used as a fluorescent protein reporter to determine the distribution and quantity of a particular target biomolecule both in vitro and in vivo, in conjunction with fluorescence microscopy techniques.

  • 基因名：
    GFP
  • 别名：
    CFP, eGFP, eYFP, GFP, GFP tag, YFP
  • 宿主：
    Mouse
  • 反应种属：
    N/A
  • 免疫原：
    Recombinant GFP Protein
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal Antibody
  • 抗体亚型：
    IgG2b
  • 纯化方式：
    >95%,Protein A purified
  • 克隆号：
    6C11C11
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    liquid
  • 应用范围：
    ELISA, WB, IF, FC, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:50000-1:6400000
    IF 1:50-1:200
    FC 1:100-1:300
    IP 1µl-2µl
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价