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  4. Avi-Tag Monoclonal Antibody

Avi-Tag Monoclonal Antibody

  • 货号：
    CSB-MA000101M0m
  • 规格：
    ￥400
  • 图片：
    • Western Blot
      Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli and Mammalian cell All lanes: Avi tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 40, 35, 30, 70, 40, 33, 35, 40 KDa
      Observed band size: 40, 35, 30, 70, 40, 33, 35, 4 KDa
      Exposure time：1min
    • Western Blot
      Positive WB detected in: 50ng Recombinant protein with Avi tag from E.coli All lanes: Avi tag antibody at 1:2000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 81.2, 67, 66.9, 66.9 KDa
      Observed band size: 82, 67, 67, 67KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: Recombinant protein from Mammalian cell with Avi tag from E.coli at 100ng, 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng All lanes: Avi tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 40 KDa
      Observed band size: 40 KDa
      Exposure time：1min
    • Western Blot
      Positive WB detected in: 50ng Recombinant protein with Avi tag from Mammalian cell Avi tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 40 KDa
      Observed band size: 40 KDa
      Exposure time：5min
    • Immunoprecipitating Avi tag in 293F whole cell lysate transfected with avi tag
      Lane 1: Mouse control IgG instead of CSB-MA000101M0m in 293F whole cell lysate transfected with avi tag. Lane 2: CSB-MA000101M0m (1µg) + 293F whole cell lysate transfected with avi tag (500µg)
      Lane 3: 293F whole cell lysate transfected with avi tag(40µg)
      For western blotting, the blot was detected with CSB-MA000101M0m at 1:1000, and a HRP-conjugated Goat anti Mouse antibody was used as the secondary antibody at 1:50000
  • 其他：

产品详情

  • 产品描述：

    The mouse is immunized with synthetic peptide GLNDIFEAQKIEWHE to obtain the B cells. Fusing mouse B cells with myeloma cells to obtain hybridomas, selecting the hybridoma cell line that produces the Avi-Tag antibody, and then culturing the selected hybridoma in the mouse abdominal cavity. The Avi-Tag monoclonal antibody is from the mouse ascites using protein G affinity chromatography, resulting in a purity of over 95%. This unconjugated IgG2a antibody is ideal for detecting the all Avi-Tagged proteins in ELISA, WB, and IP applications. The Avi-Tag is a short peptide sequence (GLNDIFEAQKIEWHE) that can be genetically encoded onto a protein of interest.

    The Avi-Tag sequence is recognized by the biotin ligase enzyme BirA, which covalently attaches biotin to a specific lysine residue within the Avi-Tag sequence. The biotinylated protein can then be easily purified or detected using streptavidin-linked reagents, which bind specifically to biotin with high affinity. This makes the Avi-Tag a useful tool for studying protein localization, interaction, and function.

  • 产品名称：
    Mouse anti-All AVI Monoclonal antibody
  • 基因名：
    AVI
  • 宿主：
    Mouse
  • 反应种属：
    All
  • 免疫原：
    Synthetic Peptide
  • 免疫原种属：
    All
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2a
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    9G3B5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:640000
    IP 2µl-5µl
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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