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  4. ACTB Monoclonal Antibody

ACTB Monoclonal Antibody

  • 货号：
    CSB-MA000091M1m
  • 规格：
    ￥400
  • 图片：
    • Western Blot
      Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissue
      All lanes: ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, NIH/3T3 whole cell lysate, Mouse kidney tissue, Rabbit kidney tissue
      All lanes: ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: Mouse liver tissue, Mouse brain tissue, Mouse lung tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse stomach tissue
      All lanes: ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: Rabbit liver tissue, Rabbit brain tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit stomach tissue, A549 whole cell lysate
      All lanes: ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: Rat liver tissue, Rat brain tissue, Rat lung tissue, Rat stomach tissue, U87 whole cell lysate
      All lanes: ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: 293T whole cell lysate at 40μg, 20μg, 10μg
      All lanes:ACTB antibody at 1:5000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • Western Blot
      Positive WB detected in: 20μg 293T whole cell lysate
      ACTB antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 42 KDa
      Observed band size: 42 KDa
      Exposure time：5min
    • IHC image of CSB-MA000091M1m diluted at 1:500 and staining in paraffin-embedded colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃ Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
    • IHC image of CSB-MA000091M1m diluted at 1:500 and staining in paraffin-embedded breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37℃ Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
    • Immunofluorescence staining of Hela cells with CSB-MA000091M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG （H+L）.
    • Immunofluorescence staining of HepG2 cells with CSB-MA000091M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG （H+L）.
    • Overlay Peak curve showing Hela cells stained with CSB-MA000091M1m (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
    • Overlay Peak curve showing HepG2 cells stained with CSB-MA000091M1m (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
  • 其他：

产品详情

  • 产品描述：

    The synthesized peptide derived from human Beta-Actin (1-50aa) is used as the immunogen to immunize the mouse. To generate the ACTB monoclonal antibody, B cells isolated from the immunized mouse are fused with myeloma cells to form hybridoma cells. The hybridoma cells that produce the ACTB antibody are then selected and cultured, and the ACTB monoclonal antibody is purified from the mouse ascites using protein A affinity chromatography. The purity of this ACTB monoclonal antibody is greater than 95%. It is highly specific to the ACTB protein from four species including human, mouse, rat, and rabbit, and can be used in five assays including ELISA, WB, IHC, IF, and FC.

    ACTB is one of the most abundant cytoskeletal proteins found in eukaryotic cells. Specifically, ACTB is involved in cell motility and cytoskeletal organization. It is one of the most widely used housekeeping genes in molecular biology and is often used as a reference gene in gene expression studies. ACTB is also commonly used as a loading control in WB experiments.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) ACTB Monoclonal antibody
  • Uniprot No.：
    P60709
  • 基因名：
  • 别名：
    ACTB;ACTB;ACTB;PS1TP5BP1;beta-ac6n;β-ac6n
  • 宿主：
    Mouse
  • 反应种属：
    Human, Mouse, Rat, Rabbit
  • 免疫原：
    A synthesized peptide derived from human Beta-Actin (1-50AA)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2b
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    1D12E12B5
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, FC
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:5000-1:80000
    IHC 1:500-1:1000
    IF 1:50-1:200
    FC 1:100-1:300
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Actin is a highly conserved protein that polymerizes to produce filaments that form cross-linked networks in the cytoplasm of cells. Actin exists in both monomeric (G-actin) and polymeric (F-actin) forms, both forms playing key functions, such as cell motility and contraction. In addition to their role in the cytoplasmic cytoskeleton, G- and F-actin also localize in the nucleus, and regulate gene transcription and motility and repair of damaged DNA.
  • 基因功能参考文献：
    1. During cell adhesion, an active multistage process naturally leads to pattern transitions from actin vortices over stars into asters. PMID: 28194011
    2. Haploinsufficiency of ACTB is the main cause of the clinical phenotype observed in the patients with 7p22.1 microdeletions. PMID: 29274487
    3. Baraitser-Winter cerebrofrontofacial syndrome is caused by missense mutations in the cytoplasmic beta- and gamma-actin genes ACTB and ACTG1. We provide an overview of the clinical characteristics (including some novel findings in four recently diagnosed patients), diagnosis, management, mutation spectrum and genetic counselling. PMID: 27625340
    4. The s describe heterozygous ACTB deletions and nonsense and frameshift mutations in 33 individuals with developmental delay, apparent intellectual disability, increased frequency of internal organ malformations (including those of the heart and the renal tract), growth retardation, and a recognizable facial gestalt. PMID: 29220674
    5. Data indicate AIM1 (absent in melanoma 1) as an actin binding protein and show that it regulates cytoskeletal remodeling and cell migration in prostate epithelial cells. PMID: 28747635
    6. Case Report: gastric pericytoma harboring the exceptionally rare translocation t(7;12) resulting in ACTB-GLI1 gene fusion. PMID: 26980027
    7. Data suggest that, in T-lymphocytes, nitric oxide generated by eNOS S-nitrosylates Cys374 on ACTB and thus regulates activation/recruitment of PRKCQ at immune synapse; S-nitrosylation of beta-actin impairs actin binding to PFN1 and regulates protein transport in lamellipodia. (eNOS = nitric oxide synthase 3; ACTB = beta-actin; PRKCQ = protein kinase C-theta; PFN1 = profilin-1) PMID: 28394935
    8. Data indicate that the IQGAP1 N-terminal fragment spanning residues 1-191 (CHDF) binds to both F-actin and Ca(2+)/calmodulin. PMID: 27798963
    9. Based on present and published dup7p22.1 patients we suggest that renal abnormalities might be an additional feature of the 7p22.1 microduplication syndrome.We also pinpoint the ACTB gene as the key gene affecting the 7p22.1 duplication syndrome phenotype. PMID: 27866048
    10. We suggest that haploinsufficiency of ACTB may be responsible for the clinical features of patients with 7p22.1 microdeletions PMID: 27633570
    11. crucial role of Glu270 in ADP-ribosylation of actin by Ia PMID: 26713879
    12. Studies indicate that the process of megakaryocyte maturation and the formation of proplatelets are essential steps in the production of mature platelets and both depend heavily on the actin and microtubule cytoskeletons. PMID: 26210823
    13. Data show that tripartite motif-containing 28 protein (TRIM28) and beta-actin were up-regulated in the glioblastoma multiforme (GBM) stem-like cells compared to the controls. PMID: 25419715
    14. Data suggest that by binding to both clathrin and F-actin, mammalian actin-binding protein 1 (mAbp1; HIP-55 or SH3P7) is specifically recruited at a late stage of clathrin-coated pits (CCPs) formation, which subsequently recruits dynamin to CCPs. PMID: 25690657
    15. The results indicate that the disease-related human beta-actin mutations p.R183W and p.E364K affect interdomain mobility and nucleotide interactions as a basis for the formation of disease phenotypes in patients. PMID: 25255767
    16. Data indicate the WASp-interacting protein (WIP)-Wiskott-Aldrich syndrome protein (WASp) interaction in the regulation of actin-dependent processes. PMID: 24962707
    17. Mutations in ACTB cause a distinctly more severe phenotype than ACTG1 mutations in Baraitser-Winter syndrome. PMID: 23756437
    18. TIA proteins can function as long-term regulators of the ACTB mRNA metabolism in mouse and human cells. PMID: 24766723
    19. Downregulation of the HuR gene results in decreased beta-actin gene expression, which in turn results in decreased motility and proliferation of corneal fibroblasts. PMID: 24826067
    20. Thus, the nucleocapsid domain in HIV-1 Gag does not appear to have a role in actin recruitment or actin incorporation into HIV-1 particles. PMID: 24789788
    21. Taken together, these findings indicate that actin filament dynamics are dispensable for HIV-1 Gag assembly at the plasma membrane of HeLa cells. PMID: 24789789
    22. Results indicate that actin cytoskeleton is one of the upstream regulators of Hippo signaling. PMID: 24040060
    23. PDI appears to regulate cytoskeletal reorganization by the thiol-disulfide exchange in beta-actin via a redox-dependent mechanism. PMID: 24415753
    24. chorein interacts with beta-adducin and beta-actin. PMID: 24129186
    25. Data indicate that monomeric actin probes concentrate in nuclear speckles. PMID: 23447706
    26. the roles of ACTB in tumors PMID: 23266771
    27. Data suggest that P-glycoprotein associate with the F-actin cytoskeleton through ezrin/radixin/moesin (ERM) in CCR9/CCL25 induced multidrug resistance of acute T-lymphocytic leukemia (T-ALL) cells. PMID: 23326330
    28. Two variants of beta-actin, beta1 and beta2 were found in the Enterovirus 71-susceptible rhabdomyosarcoma cells, compared to Enterovirus 71-resistant cells that contains only one variant beta1. PMID: 23535377
    29. Studies indicate that cofilin binds actin stoichiometrically - one cofilin molecule per actin filament subunit. PMID: 23395798
    30. Studies indicate that that vinculin not only bundles actin filaments but can also cap these filaments and promote actin polymerization. PMID: 23466368
    31. Cofilin nuclear shuttling is critical for the cofilin-actin rod stress response. PMID: 22623727
    32. These results indicate that F-actin in association with the M protein alters the interaction between the M and H proteins, thereby modulating measles virus cell-cell fusion and assembly. PMID: 23221571
    33. Data indicate beta-cytoplasmic (beta-CYA) and gamma-cytoplasmic (gamma-CYA) actins differential localization and dynamics at epithelial junctions. PMID: 22855531
    34. roles for undetected ACtB in liver cancer progression PMID: 22961449
    35. Data show that ENOA, PARK7 and Beta-actin are proper reference standards in obesity studies based on omental fat. PMID: 22272336
    36. identified de novo missense changes in the cytoplasmic actin-encoding genes ACTB and ACTG1 in one and two probands, respectively; suggest that Baraitser-Winter syndrome is the predominant phenotype associated with mutation of these two genes PMID: 22366783
    37. Recombinant human actin is constantly shuttled into the murine nucleus by importin 9 and out by exportin 6. Nuclear actin is required for maximal transcription. PMID: 22323606
    38. Antioxidant supplementation was noted to increase G6PDH in the pentose phosphate cycle and 18S rRNA in the ribosome. There were no significant changes in the gene expression levels of beta-ACT PMID: 22285204
    39. non-muscle alpha-ACTN4, HSPA5 and cytoplasmic ACTB, should be targeted in idiopathic premature ovarian failure cases PMID: 21890413
    40. Data indicate taht candidate genes ACTB, BZW, OCM, MACC1, NXPH1, PRPS1L1, RAC1 and RPA3, which lie within the DFNB90 region, were sequenced and no potentially causal variants were identified. PMID: 21734401
    41. ACTB showed high expression in forensic skin and body-fluid samples, providing a suitable marker for skin identification PMID: 21221983
    42. Nuclear beta-actin controls growth arrest of epithelial cells. PMID: 21172822
    43. Data suggest that the existence of a common epitope on the molecules of phosducin and beta-actin may reflect a topological similarity of a small region of their surfaces. PMID: 20804785
    44. Findings indicate that activation of the cofilin-F-actin pathway contributes to tumor cell migration and metastasis enhanced by Aur-A, revealing a novel function for mitotic Aur-A kinase in tumor progression. PMID: 21045147
    45. The actin network plays a role in nuclear ERalpha actions in breast cancer cells. PMID: 20308691
    46. Immunoblot analysis revealed profoundly decreased beta-actin levels during Ectromelia virus infection replicative cycle in the infected cells 24 hrs post infection. PMID: 20201613
    47. This protein has been found differentially expressed in the anterior cingulate cortex from patients with schizophrenia PMID: 20381070
    48. Data show that inducible Hox genes are selectively sensitive to the inhibition of actin polymerization and that actin polymerization is required for the assembly of a transcription complex on the regulatory region of the Hox genes. PMID: 19477923
    49. Results suggest a novel mechanism by which cofilin is regulated by v-Src through tyrosine phosphorylation that triggers the degradation of cofilin through ubiquitination-proteosome pathway and reducing cellular F-actin contents and cell spreading. PMID: 19802004
    50. region responsible for down-regulation of the gamma-actin gene during differentiation is not in the 3' end of the gene in contrast to that for beta-actin. PMID: 11787062

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  • 相关疾病：
    Dystonia, juvenile-onset (DJO); Baraitser-Winter syndrome 1 (BRWS1)
  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Nucleus.
  • 蛋白家族：
    Actin family
  • 数据库链接：

    HGNC: 132

    OMIM: 102630

    KEGG: hsa:60

    STRING: 9606.ENSP00000349960

    UniGene: Hs.520640