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V5-Tag Monoclonal Antibody

  • 货号：
    CSB-MA000160
  • 规格：
    ￥660
  • 图片：

    • IP antibody use: 5µg V5 Mouse IgG1 per ml Lysate, WB 1: 5000
      Lane 1: untransfected 293 cell lysate
      Lane 2: transfected 293 cell lysate with V5-tag fusion protein
      Lane 3: IP (untransfted 293 + anti-V5 mAb + Protein G agarose)
      Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
      Lane 5: IP (transfected 293 + anti-V5 mAb + Protein G agarose)

    • IF analysis of 293 cells transfected with a V5-tag protein,1:2000 dilution (blue DAPI, red anti-V5)

    • 1µg V5 fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000

  • 其他：

产品详情

  • 产品描述：
    V5-Tag monoclonal antibody CSB-MA000160 was produced in mouse immunized by using Synthetic Peptide as the immunogen. The V5 tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
    This V5-Tag Monoclonal Antibody was tested in the ELISA, WB, IF and IHC applications. V5-Tag is one of the epitope tags, which provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.
  • 宿主：
    Mouse
  • 反应种属：
    N/A
  • 免疫原：
    Synthetic Peptide
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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