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  4. TUBA1A Monoclonal Antibody

TUBA1A Monoclonal Antibody

  • 货号：
    CSB-MA754656A0m
  • 规格：
    ￥400
  • 图片：

    • Western Blot
      Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate,
      All lanes: TUBA1A antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
       

    • Western Blot
      Positive WB detected in: Rabbit heatrt tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue
      All lanes: TUBA1A antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
       

    • Western Blot
      Positive WB detected in: Rat brain tissue, Rat stomach tissue, Rat kidney tissue
      All lanes: TUBA1A antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
       

    • Western Blot
      Positive WB detected in: NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse brain tissue, Mouse kidney tissue
      All lanes: TUBA1A antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
       

    • Western Blot
      Positive WB detected in: Hela whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg
      All lanes: TUBA1A antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
       

    • Western Blot
      Positive WB detected in: Hela whole cell lysate
      All lanes: TUBA1A antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
       

    • Western Blot
      Positive WB detected in: Hela whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg, 0.3125µg, 0.15625µg
      All lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
       

    • Western Blot
      Positive WB detected in: Hela whole cell lysate
      All lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 320000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 52 kDa
      Observed band size: 52 kDa
       

    • IHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

    • IHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

    • Immunofluorescence staining of Hela cells with CSB-MA754656A0m at 1:75, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

    • Immunoprecipitating TUBA1A in Hela whole cell lysate
      Lane 1: Mouse control IgG (1µg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-MA754656A0m (5µg) + Hela whole cell lysate (500µg)
      Lane 3: Hela whole cell lysate (20µg)
       

    • Immunoprecipitating TUBA1A in Hela whole cell lysate
      Lane 1: Mouse control IgG (1µg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: Company A (5µg), Company B (5µg), Company C (5µg), Company D (5µg), CSB-MA754656A0m (5µg) + Hela whole cell lysate (500µg)
      Lane 3: Hela whole cell lysate (20µg)
       

    • Overlay histogram showing Hela cells stained with CSB-MA754656A0m (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

    • Overlay histogram showing Hela cells stained with Company A (5µg), Company B (5µg), Company C (5µg), Company D (5µg), CSB-MA754656A0m (5µg) (red line) at 1:150. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.

  • 其他：

产品详情

  • 产品描述：

    The product CSB-MA754656A0m is an unconjugated monoclonal antibody against the human TUBA1A. It is produced from the mouse splenocyte-myeloma cell fused hybridoma. The splenocyte is isolated from the mouse immunized with the synthesized peptide that is derived from human TUBA1A protein (297-309aa). This TUBA1A monoclonal antibody can react with TUBA1A protein from four species, including human, rabbit, rat, and mouse. Protein A-mediated purification of this TUBA1A monoclonal antibody makes its purity up to more than 95%. This antibody has been validated for use in ELISA, WB, IHC, IF, IP, and FC applications.

    TUBA1A protein plays a crucial role in the organization and stabilization of microtubules, which are important for maintaining the shape and structural integrity of cells. Additionally, TUBA1A has been shown to play a role in neuronal development and function, as it is a major constituent of the microtubules that form the axon and dendrites of neurons. Mutations in the TUBA1A gene are associated with a range of neurological disorders, including microcephaly, lissencephaly, and intellectual disability.

  • 产品名称：
    Mouse anti-Homo sapiens (Human) TUBA1A Monoclonal Antibody antibody
  • Uniprot No.：
    Q71U36
  • 基因名：
  • 别名：
    Alpha tubulin 3 antibody; Alpha-tubulin 3 antibody; B alpha 1 antibody; FLJ25113 antibody; LIS3 antibody; TBA1A_HUMAN antibody; TUBA1A antibody; TUBA3 antibody; Tubulin alpha 1a antibody; Tubulin alpha 1A chain antibody; Tubulin alpha 3 antibody; Tubulin alpha 3 chain antibody; Tubulin alpha brain specific antibody; Tubulin alpha-1A chain antibody; Tubulin alpha-3 chain antibody; Tubulin B alpha 1 antibody; Tubulin B-alpha-1 antibody
  • 宿主：
    Mouse
  • 反应种属：
    Human, Rabbit, Rat, Mouse
  • 免疫原：
    A synthesized peptide derived from human Tubulin alpha-1A chain (297-309aa)
  • 免疫原种属：
    Homo sapiens (Human)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal Antibody
  • 抗体亚型：
    IgG2a
  • 纯化方式：
    >95%, Protein A purified
  • 克隆号：
    7E5C12
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IHC, IF, FC, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:20000-1:320000
    IHC 1:100-1:300
    IF 1:50-1:200
    FC 1:100-1:300
    IP 1µg-5µg
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • 基因功能参考文献：
    1. A de novo heterozygous c.320A>G [p.(His 107 Arg)] mutation in TUBA1A was identified in a patient with microcephaly, epileptic seizures, and severe developmental delay. PMID: 29109381
    2. Given that Spastin engages the MT in two places, we propose that severing occurs by forces exerted on the C-terminal tail of tubulin, which results in a conformational change in tubulin, which releases it from the polymer. PMID: 17389232
    3. Molecular docking studies revealed that 6f interacted and bound ef fi ciently with the colchicine-binding site of tubulin. In addition, 6f treatment induced G2/M cell cycle arrest dose-dependently and subsequently induced cell apoptosis PMID: 28440465
    4. induced pluripotent stem cells (iPSCs) from the umbilical cord and peripheral blood of two lissencephaly patients with different clinical severities carrying alpha tubulin (TUBA1A) missense mutations, were generated. PMID: 27431206
    5. Long intergenic non-coding RNA APOC1P1-3 inhibits apoptosis by decreasing alpha-tubulin acetylation in breast cancer. PMID: 27228351
    6. Results show that Tuba1a plays an essential, noncompensated role in neuronal saltatory migration in vivo and highlight the importance of microtubule flexibility in nucleus-centrosome coupling and neuronal-branching regulation during neuronal migration. PMID: 28687665
    7. data suggest that the TUBA1A mutations disrupting lateral interactions have pronounced dominant-negative effects on microtubule dynamics that are associated with the severe end of the lissencephaly spectrum PMID: 26493046
    8. Data show that tubulin phosphorylation and acetylation play important roles in the control of microtubule assembly and stability. PMID: 26165356
    9. Data show that plasma membrane Ca(2+)-ATPase (PMCA) was associated with tubulin in normotensive and hypertensive erythrocytes. PMID: 26307527
    10. Studies indicate that alpha-tubulin acetylation and microtubule level is mainly governed by opposing actions of alpha-tubulin acetyltransferase 1 (ATAT1) and histone deacetylase 6 (HDAC6). PMID: 26227334
    11. Data from studies using peptide fragment of alpha-tubulin (residues 31-49) suggest that Ser38 is crucial for substrate recognition by alpha-tubulin acetylase 1 (ATAT1); Asp39, Ile42, the glycine stretch (residues 43-45), and Asp46 are also involved. PMID: 25602620
    12. Lysine 40 acetylation of alpha-tubulin does not result in significant changes in kinesin-1's landing rate or motility parameters. PMID: 24940781
    13. These results demonstrated that SelP interacts with tubulin, alpha 1a (TUBA1A). PMID: 24914767
    14. This study show all foetuses with lissencephaly and cerebellar hypoplasia carried distinct TUBA1A mutations. PMID: 25059107
    15. These findings call attention to PKC-mediated phosphorylation of alpha-tubulin as a novel mechanism for controlling the dynamics of microtubules that result in cell movement. PMID: 24574051
    16. case provides new insight into the wide spectrum of disease phenotypes associated with TUBA1A mutation PMID: 23528852
    17. The present study confirms that mutations in tubulin genes are responsible for complex brain malformation. PMID: 24392928
    18. Studies suggest that tubulin-interactive agents have the potential to play a significant role in the fight against cancer. PMID: 23818224
    19. Missense mutations in TUBA1A were found in 3 patients with polymicrogyria. PMID: 22948023
    20. We described the clinical course and pathological findings in a child with TUBA1A mutation PMID: 22633752
    21. TUBA1A and TUBB2B coding regions have been sequenced that are associated with cortical malformations. PMID: 23361065
    22. Data show that Na(+),K(+)-ATPase activity was >50% lower and membrane-associated tubulin content was >200% higher in erythrocyte membranes from diabetic patients. PMID: 22565168
    23. study describes a 14-month-old girl with TUBA1A mutation-associated lissencephaly, and summarize the clinical and neuroradiologic findings of 19 cases in the literature PMID: 22264709
    24. Alpha2B-adrenergic receptor interaction with tubulin controls its transport from the endoplasmic reticulum to the cell surface PMID: 21357695
    25. The expression of alpha-tubulin and MDR1 may play an important role in the development and progression of human non-small cell lung carcinoma. PMID: 20510079
    26. We report a mutation in TUBA1A as a cause of polymicrogyria. So far, all mutations in TUBA1A have occurred de novo, resulting in isolated cases. This article describes familial recurrence of TUBA1A mutations due to somatic mosaicism in a parent. PMID: 21403111
    27. Data show that IAV-infected cells contain elevated level of AcTub and alpha-tubulin. PMID: 21094644
    28. Mutations in TUBA1A result in defects in the tubulin folding and heterodimer assembly. PMID: 20603323
    29. LIS-associated mutations of TUBA1A operate via diverse mechanisms that include disruption of binding sites for microtubule-associated proteins. PMID: 20466733
    30. the dipole moments of each tubulin isotype may influence their functional characteristics within the cell, resulting in differences for MT assembly kinetics and stability PMID: 16941085
    31. Mutations in alpha-tubulin in mice and humans that affect neuronal migration result in abnormal lamination of brain structures with associated behavioral deficits. PMID: 17218254
    32. Retrospective examination of MR images suggests that patients with TUBA1A mutations share not only cortical dysgenesis, but also cerebellar, hippocampal, corpus callosum, and brainstem abnormalities PMID: 17584854
    33. Increased expression of tubulin alpha is associated with pulmonary sclerosing hemangioma PMID: 17914564
    34. The diminished production of TUBA1A tubulin in R264C individuals is consistent with haploinsufficiency as a cause of the disease phenotype. PMID: 18199681
    35. the TUBA1A phenotype is distinct from LIS1, DCX, RELN and ARX lissencephalies. Compared with the phenotypes of children mutated for TUBA1A, these prenatally diagnosed fetal cases occur at the severe end of the TUBA1A lissencephaly spectrum. PMID: 18669490
    36. Missense mutations within the TUBA1A gene are associated with specific abnormalities in lissencephaly. PMID: 18728072
    37. mutation analysis in the TUBA1A gene in 46 patients with classical lissencephaly. PMID: 18954413
    38. This protein has been found differentially expressed in the Wernicke's Area from patients with schizophrenia. PMID: 19405953

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  • 相关疾病：
    Lissencephaly 3 (LIS3)
  • 亚细胞定位：
    Cytoplasm, cytoskeleton.
  • 蛋白家族：
    Tubulin family
  • 组织特异性：
    Expressed at a high level in fetal brain.
  • 数据库链接：

    HGNC: 20766

    OMIM: 602529

    KEGG: hsa:7846

    STRING: 9606.ENSP00000301071

    UniGene: Hs.654422