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MBP Monoclonal Antibody

  • 货号：
    CSB-MA000061M0m
  • 规格：
    ￥400
  • 图片：
    • MBP-tagged fusion protein(20ng/ml) was subjected to SDS-PAGE followed by Western Blot with CSB-MA000061M0m at dilution of
      Lane 1:1000 Lane 4:8000 Lane 7:64000
      Lane 2:2000 Lane 5:16000 Lane 8:128000
      Lane 3:4000 Lane 6:32000 Lane 9:256000
      Secondary
      Goat polyclonal to Mouse IgG at 1/5000 dilution
      Predicted band size:50kd
      Observed band size:50kd
  • 其他：

产品详情

  • 产品描述：

    The MBP monoclonal antibody is produced by immunizing mice with the MBP-Tag fusion protein and then fusing B cells isolated from the immunized mice with myeloma cells to create hybridomas. After screening, the best hybridoma cell line is cultured in the mouse abdominal cavity. The MBP monoclonal antibody is purified from the mouse ascites using protein G affinity chromatography to ensure a purity of over 95%. This unconjugated IgG2a antibody is ideal for detecting MBP-tagged proteins in ELISA and WB applications.

    MBP is a cytoplasmic protein that binds specifically to maltose and other sugar molecules. It is commonly used as a fusion partner for recombinant protein expression and purification, allowing for efficient purification of the fused protein using immobilized metal ion affinity chromatography (IMAC) or amylose resin. MBP has also been shown to have chaperone activity and can enhance the solubility and stability of fusion proteins. In addition, MBP has been used as a tool in studies of protein-protein interactions and protein folding.

  • 别名：
    Maltose Binding Protein, MBP, MBP Tag
  • 宿主：
    mouse
  • 反应种属：
    N/A
  • 免疫原：
    MBP-Tag fusion protein
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    monoclonal
  • 抗体亚型：
    IgG2a
  • 纯化方式：
    >95%,Protein G purified
  • 克隆号：
    1E52C2
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    liquid
  • 应用范围：
    ELISA,WB
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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