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  4. His-Tag Monoclonal Antibody

His-Tag Monoclonal Antibody

  • 货号：
    CSB-MA000159
  • 规格：
    ￥660
  • 图片：

    • IP antibody use: 5µg His Mouse IgG1 per ml Lysate, WB 1:3000
      Lane 1: untransfected 293 cell lysate
      Lane 2: transfected 293 cell lysate with His-tag fusion protein
      Lane 3: IP (untransfected 293 + anti-His mAb + Protein G agarose)
      Lane 4: IP (transfected 293 + normal Mouse IgG + Protein G agarose)
      Lane 5: IP (transfected 293 + anti-His mAb + Protein G agarose)
      Lane 6: Recombinant protein (E.coli)

    • IF analysis of 293 cells transfected with a His-tag protein,1:1000 dilution (blue DAPI, red anti-His)

    • 2µg His fusion protein + Primary antibody dilution at 1) 1:5000 2) 1:10000

  • 其他：

产品详情

  • 产品描述：
    His-Tag monoclonal antibody CSB-MA000159 was produced in mouse immunized by using Synthetic Peptide as the immunogen. The immunogen is a short peptide. It is specifically designed for the adsorptive purification of recombinant proteins. The relatively small size of His tags makes its integration into expression vectors extremely easy.
    This His-Tag Monoclonal Antibody was tested in the WB, IF, IP and ELISA applications. An efficient detection and purification system based on fusion proteins can be established using the His tag. It can be used for detection and expression of His-tag fusion expression proteins, intracellular localization, and purification, qualitative or quantitative detection of His fusion-expressed proteins and the like. It doesn’t have species restricted.
  • 宿主：
    Mouse
  • 反应种属：
    N/A
  • 免疫原：
    Synthetic Peptide
  • 标记方式：
    Non-conjugated
  • 抗体亚型：
    IgG
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:500-1:5000
    IF 1:50-1:200
    IP 1:200-1:2000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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