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  4. Flag Tag Monoclonal Antibody

Flag Tag Monoclonal Antibody

  • 货号：
    CSB-MA000021M0m
  • 规格：
    ￥400
  • 图片：

    • Western Blot
      Positive WB detected in: 10ng Flag Tag fusion protein Flag Tag antibody at 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa
       

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.625ng, 1.5625ng, 0.78ng
      All lanes: Flag Tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa
       

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein 1, 2, 3, 4 at 50ng
      All lanes: Flag Tag antibody at 1:1000
      Secondary
      Goat polyclonal to mouse IgG at 1/50000 dilution
      Predicted band size: 59.6, 36, 35, 42 kDa
      Observed band size: 59.6, 36, 35, 42 kDa
       

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein at 10ng
      All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:50000, 1:100000, 1:200000, 1:400000, 1:800000, 1:1600000, 1:3200000, 1:6400000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa
       

    • Western Blot
      Positive WB detected in: Flag Tag fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng, 0.78ng, 0.39ng
      All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa
       

    • Western Blot
      Positive WB detected in: 5 different recombinant proteins with Flag Tag
      All lanes: Company A, Company B, Company C, Company D, CSB-MA000021M0m antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/50000 dilution
       

    • Immunoprecipitating Flag Tag in Transfected HEK-293 cell whole cell lysate
      Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Transfected HEK-293 cell whole cell lysate
      Lane 2: CSB-MA000021M0m (1µl) + Transfected HEK-293 cell whole cell lysate (500µg)
      Lane 3: Transfected HEK-293 cell whole cell lysate (20µg)
      For western blotting, the blot was detected with CSB-MA000021M0m at 1:5000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
       

    • Immunoprecipitating Flag Tag in 293F transfected whole cell lysate
      Lane 1: Mouse control IgG instead of CSB-MA000021M0m in Flag Tag in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: Company A (1µl), Company B (1µl), Company C (1µl), Company D (1µl), CSB-MA000021M0m (1µl) + Flag Tag in 293F transfected whole cell lysate (500µg)
      Lane 3: Flag Tag in 293F transfected whole cell lysate (20µg)

    • Immunofluorescence staining of 293T cells transfected with Flag tag by CSB-MA000021M0m at 1:300, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-mouse IgG(H+L). The image on the right is the 293T cells transfected without Flag tag.

  • 其他：

产品详情

  • 产品描述：
    Flag Tag monoclonal antibody CSB-MA000021M0m was produced in the mouse immunized by using the DYKDDDDK(Flag) synthetic peptide conjugate to KLH as the immunogen. The Flag tag, a popular short peptide tag (DYKDDDDK), can be fused to the C- terminus or N-terminus of the protein. It is used in recombinant DNA technology and can be used for affinity chromatography and can also be used with other affinity tags.
    This Flag Tag Monoclonal Antibody was tested in the WB, IF, IP and ELISA applications. The non-conjugated IgG2a got purified by protein G and reached up to 95% in purity. Flag antibodies can be used to detect and express the expression of Flag-tagged fusion proteins, intracellular localization, and to purify, qualitatively or quantitatively detect Flag fusion-expressed proteins. It doesn’t have species restricted.
  • 别名：
    DDK tag, DYKDDDDK, Flag, Flag Tag
  • 宿主：
    Mouse
  • 反应种属：
    All
  • 免疫原：
    DYKDDDDK(Flag) synthetic peptide conjugate to KLH
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    IgG2a
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    4G5C8
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IF, IP
  • 推荐稀释比：
    Application Recommended Dilution
    WB 1:1000-1:10000
    IF 1:100-1:300
    IP 0.5ul-2ul
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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