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  4. E-Tag Monoclonal Antibody

E-Tag Monoclonal Antibody

  • 货号：
    CSB-MA000151M0m
  • 规格：
    ￥400
  • 图片：

    • Western Blot
      Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
      All lanes: E-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 36 kDa
      Observed band size: 36 kDa
       

    • Western Blot
      Positive WB detected in: E-tagged fusion protein
      All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 36 kDa
      Observed band size: 36 kDa
       

    • Immunoprecipitating E-Tag in 293F transfected whole cell lysate
      Lane 1: Mouse control IgG (1µg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
      Lane 2: CSB-MA000151M0m (5µg) + 293F transfected whole cell lysate (500µg)
      Lane 3: 293F transfected whole cell lysate (20µg)

       

    • ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)
      Antigen at dilution of 1µg/ml
      E-Tag antibody at (0-0.15µg)
      Goat polyclonal to Mouse IgG at 1/50000 dilution

       

    • Western Blot
      Positive WB detected in: 1-3 lanes: E‐tagged fusion protein, 4-5 lanes: Recombinant protein without E-tagged, 6 lane: BSA-E-Tag
      All lanes: E-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 30, 68, 55, 67-157kDa
      Observed band size: 30, 68, 55, 67-157kDa

  • 其他：

产品详情

  • 产品描述：

    The monoclonal anti-E-Tag (IgG1 isotype) antibody is produced from the hybridomas fused by the myeloma cells and mouse splenocytes. The splenocytes are isolated from the mouse immunized with the E-tag synthetic peptide conjugated to KLH. This unconjugated E-Tag monoclonal antibody is purified through protein G with a purity of more than 95%. It can react with all E-Tag-fused proteins. And it is amenable to ELISA, WB, and IP applications.

    E-Tag is a short amino acid sequence (GAPVPYPDPLEPR) that is frequently used as an epitope tag in molecular biology experiments to facilitate the detection and purification of recombinant proteins. The E-Tag sequence is genetically fused to the protein of interest, and antibodies or affinity resins that specifically recognize the E-Tag can be used to detect or purify the tagged protein. This allows researchers to study the expression, localization, and function of the protein in cells or tissues.

  • 别名：
    E-Tag
  • 宿主：
    Mouse
  • 反应种属：
    All
  • 免疫原：
    GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal Antibody
  • 抗体亚型：
    IgG1
  • 纯化方式：
    >95%, Protein G purified
  • 克隆号：
    11H12B3
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, IP
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:4000-1:8000
    WB 1:1000-1:320000
    IP 1μg-5μg
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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