SARS-CoV-2抗体
新冠病毒引起的新冠肺炎疫情此起彼伏，对全球的医疗卫生系统和经济带来了巨大影响，同时也给新冠疫苗、治疗药物以及诊断带来了新的挑战。武汉j9九游会登录入口首页生物一直密切关注新冠病毒的进化情况，目前已开发了大量高质量SARS-CoV-2相关抗体试剂，包括N抗体、S抗体、S RBD纳米抗体、N抗体对和S RBD抗体对等。
其中，N抗体与S抗体可用于新冠病毒致病性机理研究，可通过WB定性检测新冠抗原；N抗体对和S RBD抗体对可用于新冠试剂盒的开发；另外，部分抗体验证了中和试验和主流突变株检测，可作为质控品，应用于针对突变株的中和抗体筛选、重组蛋白疫苗研发、疫苗接种后血清中的中和抗体检测等。
j9九游会登录入口首页特色
- 抗体试剂类型多
- 高特异性
- 多种应用场景验证
- 多个主流突变株验证
部分应用场景及验证数据
● WB定性抗原检测
Positive WB detected in: His tag-tagged SARS-CoV-2 N recombinant protein (CSB-EP3325GMY); SARS-CoV-2 N antibody at 1:1000 (CSB-RA33255A1GMY)
Positive WB detected in: SARS-CoV-2 N recombinant protein (CSB-EP3325GMY) at 40ng, 20ng, 10ng; All lanes N antibody (CSB-MA33255A0m) at 1:1000
● 功能性ELISA验证抗原-抗体结合活性
Immobilized SARS-CoV-2-N (CSB-EP3325GMY) at 2 μg/mL can bind SARS-CoV-2-N (CSB-RA33255A0GMY) Antibody, the EC50 is 6.892 ng/mL.
Immobilized SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) at 2 μg/ml can bind SARS-CoV-2-S Antibody (CSB-MA33245A0m), the EC50 is 32.59 to 39.33 ng/mL.
● 用于胶体金（GICA）快速检测
In the GICA detection system, the background of antibody (CSB-RA33255A2GMY) is clean, the detection limit can be as low as 15.625ng/mL (1.09ng/0.07mL), and the sensitivity is very good.
In the GICA detection system, the background of antibody (CSB-RA33245A0GMY) is clean, the detection limit can be as low as 13.94ng/mL (0.976ng/0.07mL), and the sensitivity is very good.
● 抗体对经S-ELISA验证
SARS-CoV-2 N Antibody Pair 1 (CSB-EAP33255A1) is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA).
SARS-CoV-2 Spike RBD Antibody Pair 1 (CSB-EAP33245) is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA).
● 经中和试验验证中和抗体
Binding signal of SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) and ACE2 protein-HRP conjugate (CSB-MP866317HU) was inhibited by S Antibody (CSB-RA33245A1GMY) with the IC50 is 2.38 μg/mL
Binding signal of SARS-CoV-2-S1-RBD (CSB-YP3324GMY1) and ACE2-HRP (CSB-YP3324GMY1) was inhibited by SARS-CoV-2 Spike RBD Nanobody (CSB-RA33245A2GMY) with the IC50 is 0.1074 μg/mL.
● 多个突变株识别验证
| Protein Code | Variants | EC50 (ng/mL) |
|---|---|---|
| CSB-EP3325GMY(M1) | D3L, R203K, G204R, S235F | 2. 562-3.421 |
| CSB-EP3325GMY(M2) | T205I | 29.34-40.67 |
| CSB-EP3325GMY(M3) | P80R | 63.78-113.6 |
| CSB-EP3325GMY(M4) | P13L | 2.825-4.124 |
| CSB-EP3325GMY(M5) | D103Y | 7.358-9.976 |
| CSB-EP3325GMY(M8) | S202N | 1.466-2.382 |
| CSB-EP3325GMY(M9) | L230F | 4.854-7.721 |
| CSB-EP3325GMY(M10) | I292T | 3.859-7.358 |
| CSB-EP3325GMY(M11) | Q384H | 2.785-4.100 |
| CSB-EP3325GMY(M12) | R203K, G204R | 4.664-8.256 |
| CSB-EP3325GMY(M13) | R203M, D377Y | 0.8488-1.385 |
| CSB-EP3325GMY(M14) | D63G, R203M, D377Y | 1.304-1.875 |
The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/mL can bind N Recombinant Antibody (CSB-RA33255A1GMY), the EC50 of N Recombinant Antibody.
| Protein Code | Variants | EC50 (ng/mL) |
|---|---|---|
| CSB-MP3324GMY1(M8) | E484K | 371.0-487.5 |
| CSB-MP3324GMY1(M7) | K417N | 4.522-5.950 |
| CSB-MP3324GMY1(M6) | N501Y | 47.09-86.25 |
| Protein Code | Variants | EC50 (ng/mL) |
|---|---|---|
| CSB-MP3324GMY1(M9) | K417T | 8.706-13.61 |
| CSB-MP3324GMY1(M10) | K417N, E484K, N501Y | 97.92-169.6 |
| CSB-MP3324GMY(M1) | D614G | 23.19-34.02 |
The Binding Activity of S protein with S Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized S at 2 μg/mL can bind S Recombinant Antibody, the EC50 of S Recombinant Antibody (CSB-RA33245A1GMY).
产品列表
● N antibody
| 货号 | 产品名称 | 反应种属 | 应用类型 |
|---|---|---|---|
| CSB-RA33255A1GMY | N Antibody | SARS-CoV-2 | ELISA, WB, GICA |
| CSB-RA33255A2GMY | N Antibody | SARS-CoV-2 | ELISA |
| CSB-MA33255A0m | N Antibody | GICA | Mouse |
| CSB-PA33254A0Rb | N Antibody | GICA | Rabbit |
| CSB-RA33255A0GMY | N Antibody | SARS-CoV-2 | ELISA, GICA |
| CSB-RA33255B1GMY | N Antibody, HRP conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33255C1GMY | N Antibody, FITC conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33255D1GMY | N Antibody, Biotin conjugated | SARS-CoV-2 | ELISA |
| CSB-MA33255A1m | N Antibody | GICA | Mouse |
| CSB-MA33255A2m | N Antibody | GICA | Mouse |
| CSB-PA33254B0Rb | N Antibody, HRP conjugated | SARS-CoV-2 | ELISA |
| CSB-PA33254C0Rb | N Antibody, FITC conjugated | SARS-CoV-2 | ELISA |
| CSB-PA33254D0Rb | N Antibody, Biotin conjugated | SARS-CoV-2 | ELISA |
● S antibody
| 货号 | 产品名称 | 反应种属 | 应用类型 |
|---|---|---|---|
| CSB-RA33245A1GMY | S Antibody | SARS-CoV-2 | ELISA, GICA, Neutralising |
| CSB-MA33245A2m | S Monoclonal Antibody | GICA | Mouse |
| CSB-PA33245YA01GMY | S Antibody | GICA | Rabbit |
| CSB-RA33245A0GMY | S Antibody | SARS-CoV-2 | ELISA, GICA |
| CSB-RA33245B1GMY | S Antibody, HRP conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33245C1GMY | S Antibody, FITC conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33245D1GMY | S Antibody, Biotin conjugated | SARS-CoV-2 | ELISA |
| CSB-MA33245A0m | S Monoclonal Antibody | GICA | Mouse |
| CSB-MA33245A1m | S Monoclonal Antibody | GICA | Mouse |
| CSB-PA33245YB01GMY | S Antibody | SARS-CoV-2 | ELISA |
| CSB-PA33245YC01GMY | S Antibody | SARS-CoV-2 | ELISA |
| CSB-PA33245YD01GMY | S Antibody | SARS-CoV-2 | ELISA |
| CSB-PA33245YA11GMY | S Antibody | GICA | Rabbit |
| CSB-PA33245YB11GMY | S Antibody | SARS-CoV-2 | ELISA |
| CSB-PA33245YC11GMY | S Antibody | SARS-CoV-2 | ELISA |
| CSB-PA33245YD11GMY | S Antibody | SARS-CoV-2 | ELISA |
● N Antibody Pair
| 货号 | 产品名称 | 反应种属 | 应用类型 |
|---|---|---|---|
| CSB-EAP33255A2 | SARS-CoV-2 N Antibody Pair 2 | SARS-CoV-2 | S-ELISA |
| CSB-EAP33255A1 | SARS-CoV-2 N Antibody Pair 1 | SARS-CoV-2 | S-ELISA |
| CSB-EAP33255A3 | SARS-CoV-2 N Antibody Pair 3 | SARS-CoV-2 | S-ELISA |
| CSB-EAP33255A4 | SARS-CoV-2 N Antibody Pair 4 | SARS-CoV-2 | S-ELISA |
● S RBD Nanobody Antibody
| 货号 | 产品名称 | 反应种属 | 应用类型 |
|---|---|---|---|
| CSB-RA33245A2GMY | SARS-CoV-2 RBD Nanobody | SARS-CoV-2 | ELISA, GICA, Neutralising |
| CSB-RA33245B2GMY | SARS-CoV-2 RBD Nanobody, HRP conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33245C2GMY | SARS-CoV-2 RBD Nanobody, FITC conjugated | SARS-CoV-2 | ELISA |
| CSB-RA33245D2GMY | SARS-CoV-2 RBD Nanobody, Biotin conjugated | SARS-CoV-2 | ELISA |
● S RBD Antibody Pair
| 货号 | 产品名称 | 反应种属 | 应用类型 |
|---|---|---|---|
| CSB-EAP33245 | SARS-CoV-2 Spike RBD Antibody Pair 1 | SARS-CoV-2 | S-ELISA |
