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N Recombinant Monoclonal Antibody

  • 货号：
    CSB-RA33255A1GMY
  • 规格：
    ￥1540
  • 图片：
    • Western Blot
      Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli (CSB-EP3325GMY)
      SARS-CoV-2 nucleocapsid antibody at 1:1000 (CSB-RA33255A1GMY)
      Secondary
      Peroxidase-Affinipure Goat Anti-Human IgG Fc Fragment Specific at 1/20000 dilution
      Predicted band size: 48 kDa
      Observed band size: 55 kDa
    • The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
      Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 1.586 ng/ml.
    • In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A1GMY) is clean, the detection limit can be as low as 125ng/ml (8.75ng/0.07ml), and the sensitivity is very good.
    • ELISA: Immobilize various types of SARS proteins at concentration of 2μg/ml on solid substrate, then react with SARS-CoV-2-N Antibody at concentration of 100μg/ml, 10μg/ml and 1μg/ml. It shows the SARS-CoV-2-N Antibody (CSB-RA33255A1GMY) is specific for SARS-CoV-2-N protein, without any cross-reactivity with NL63-CoV, HCoV-OC43, HCoV-229E or HCoV-HKU1.
    • The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.
    • The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.
    • The Binding Activity of N protein with N Recombinant Antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized N at 2 μg/ml can bind N Recombinant Antibody, the EC50 of N Recombinant Antibody.
  • 其他：

产品详情

  • 产品描述：

    Recombinant anti-SARS-CoV-2 Nucleoprotein (N protein) Mouse single-chain variable fragment (ScFv) is expressed from 293 cells (HEK293) with a human IgG1 Fc tag on C-terminal. The resulting product is the recombinant SARS-CoV-2 N monoclonal antibody. This N antibody is recommended for ELISA, WB, and GICA applications. It has undergone affinity-chromatography purification. And it can detect the endogenous content of human SARS-Cov-2 N protein.

    The SARS-CoV-2 N protein is an abundant RNA-binding protein. It contains two structured domains: the RNA binding domain and the oligomerization domain, which are separated by a long flexible linker. The separated domains allow the N protein to execute many functions in the viral lifecycle such as RNA replication, virion assembly, and immune system interference.

  • Uniprot No.：
    P0DTC9
  • 别名：
    N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
  • 反应种属：
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 免疫原：
    Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)
  • 免疫原种属：
    Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
  • 标记方式：
    Non-conjugated
  • 克隆类型：
    Monoclonal
  • 抗体亚型：
    Mouse scFv fusion with human IgG1 Fc
  • 纯化方式：
    Affinity-chromatography
  • 克隆号：
    1A6
  • 浓度：
    It differs from different batches. Please contact us to confirm it.
  • 保存缓冲液：
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
  • 产品提供形式：
    Liquid
  • 应用范围：
    ELISA, WB, GICA
  • 推荐稀释比：
    Application Recommended Dilution
    ELISA 1:10000-1:50000
    WB 1:500-1:5000
    GICA 1:1000-1:10000
  • Protocols：
  • 储存条件：
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期：
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

产品评价

靶点详情

  • 功能：
    Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
  • 亚细胞定位：
    Virion. Host cytoplasm.
  • 蛋白家族：
    Betacoronavirus nucleocapsid protein family