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  4. Recombinant Vesicular stomatitis Indiana virus Nucleoprotein (N)

Recombinant Vesicular stomatitis Indiana virus Nucleoprotein (N)

  • 中文名称：
    Recombinant Vesicular stomatitis Indiana virus Nucleoprotein(N),Yeast
  • 货号：
    CSB-YP758857VBF
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Vesicular stomatitis Indiana virus Nucleoprotein(N),Yeast
  • 货号：
    CSB-EP758857VBF
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Vesicular stomatitis Indiana virus Nucleoprotein(N),Yeast
  • 货号：
    CSB-EP758857VBF-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Vesicular stomatitis Indiana virus Nucleoprotein(N),Yeast
  • 货号：
    CSB-BP758857VBF
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Vesicular stomatitis Indiana virus Nucleoprotein(N),Yeast
  • 货号：
    CSB-MP758857VBF
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    NNucleoprotein; NP; Nucleocapsid protein; Protein N
  • 种属：
    Vesicular stomatitis Indiana virus (strain 98COE North America) (VSIV)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-422
  • 氨基酸序列
    MSVTVKRIID NTVIVPKLPA NEDPVEYPAD YFRKSKEIPL YINTTKSLSD LRGYVYQGLK SGNVSIIHVN SYLYGALKDI RGKLDKDWSS FGINIGKAGD TIGIFDLVSL KALDGVLPDG VSDASRTSAD DKWLPLYLLG LYRVGRTQMP EYRKKLMDGL TNQCKMINEQ FEPLVPEGRD IFDVWGNDSN YTKIVAAVDM FFHMFKKHEC ASFRYGTIVS RFKDCAALAT FGHLCKITGM STEDVTTWIL NREVADEMVQ MMLPGQEIDK ADSYMPYLID FGLSSKSPYS SVKNPAFHFW GQLTALLLRS TRARNARQPD DIEYTSLTTA GLLYAYAVGS SADLAQQFCV GDNKYTPDDS TGGLTTNAPP QGRDVVEWLG WFEDQNRKPT PDMMQYAKRA VMSLQGLREK TIGKYAKSEF DK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Encapsidates the genome in a ratio of one N per nine ribonucleotides, protecting it from nucleases. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. Replication is dependent on intracellular concentration of newly synthesized N, termed N(0), which corresponds to the protein not associated with RNA. In contrast, when associated with RNA, it is termed N. During replication, encapsidation by N(0) is coupled to RNA synthesis and all replicative products are resistant to nucleases.
  • 基因功能参考文献：
    1. These results demonstrate that N-terminal phosphorylation is indispensable for P to prevent N from binding to nonviral RNAs and to maintain the N-specific encapsidation of viral genomic RNA for functional template formation. PMID: 23283948
    2. determined a 2.9 angstrom structure of a complex containing 10 molecules of the N protein and 90 bases of RNA; the RNA is tightly sequestered in a cavity at the interface between two lobes of the N protein PMID: 16778022
    3. determination whether G protein or M protein was colocalized with VSV nucleocapsid protein (N protein) outside the budding sites PMID: 18367537
  • 亚细胞定位：
    Virion. Host cytoplasm.
  • 蛋白家族：
    Vesiculovirus nucleocapsid protein family
  • 数据库链接：

    KEGG: vg:1489831