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  4. Recombinant Salmonella typhimurium Motility protein B (motB), partial

Recombinant Salmonella typhimurium Motility protein B (motB), partial

  • 中文名称：
    鼠伤寒沙门菌motB重组蛋白
  • 货号：
    CSB-YP345913SXB
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    鼠伤寒沙门菌motB重组蛋白
  • 货号：
    CSB-EP345913SXB
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    鼠伤寒沙门菌motB重组蛋白
  • 货号：
    CSB-EP345913SXB-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    鼠伤寒沙门菌motB重组蛋白
  • 货号：
    CSB-BP345913SXB
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    鼠伤寒沙门菌motB重组蛋白
  • 货号：
    CSB-MP345913SXB
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    motB
  • Uniprot No.：
  • 别名：
    motB; STM1922; Motility protein B; Chemotaxis protein MotB
  • 种属：
    Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    MotA and MotB comprise the stator element of the flagellar motor complex. Required for the rotation of the flagellar motor. Might be a linker that fastens the torque-generating machinery to the cell wall.
  • 基因功能参考文献：
    1. results suggest that the MotA/B complex is a load-sensitive proton channel that efficiently couples proton translocation with the conformational changes that generate torque PMID: 24255940
    2. The proton-conductivity of a Salmonella MotA/B complex not incorporated into the motor is two orders of magnitude lower than that of a complex that is incorporated and activated. PMID: 20178785
    3. MotB(C) dimer domain plays an important role in targeting and stable anchoring of the MotA/MotB complex to putative stator- PMID: 18310339
    4. These results suggest that second-site mutations recover a torque generation step involving stator-rotor interactions coupled with protonation/deprotonation of Glu-33 but not the maximum proton conductivity of MutB. PMID: 18723617
    5. MotBC dimer formation is crucial to functional stator formation. Dimerization through the PGB domain is essential for proper arrangement of the proton channel. PMID: 19627504

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  • 亚细胞定位：
    Cell inner membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    MotB family
  • 数据库链接：

    KEGG: stm:STM1922

    STRING: 99287.STM1922