Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Tryptophan 5-hydroxylase 1 (Tph1)

Recombinant Rat Tryptophan 5-hydroxylase 1 (Tph1)

  • 货号：
    CSB-YP024100RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP024100RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP024100RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP024100RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP024100RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Tph1
  • Uniprot No.：
  • 别名：
    Tph1; Tph; Tryptophan 5-hydroxylase 1; EC 1.14.16.4; Tryptophan 5-monooxygenase 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-444
  • 氨基酸序列
    MIEDNKENKD HSSERGRVTL IFSLKNEVGG LIKALKIFQE NHVNLLHIES RKSKRRNSEF EIFVDCDINR EQLNDIFPLL KSHTTVLSVD SPDQLPEKED VMETVPWFPK KISDLDFCAN RVLLYGSELD ADHPGFKDNV YRRRRKYFAE LAMNYKHGDP IPKIEFTEEE IKTWGTIFRE LNKLYPTHAC REYLRNLPLL SKYCGYREDN VPQLEDVSNF LKERTGFSIR PVAGYLSPRD FLSGLAFRVF HCTQYVRHSS DPLYTPEPDT CHELLGHVPL LAEPSFAQFS QEIGLASLGA SEETVQKLAT CYFFTVEFGL CKQDGQLRVF GAGLLSSISE LRHALSGHAK VKPFDPKVAC KQECLITSFQ DVYFVSESFE DAKEKMREFA KTVKRPFGVK YNPYTQSIQV LRDSKSITSA MNELRHDLDV VNDALARVSR WPSV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.
  • 基因功能参考文献：
    1. Ursolic acid derivatives targeted to tryptophan hydroxylase 1 prevented bone loss by suppressing serotonin biosynthesis without estrogenic side-effects in ovariectomized rats. PMID: 25930119
    2. there exists possible interrelation between tyrosine hydroxylase and TPH gene expression and epigenetic histone acetylation in chronic unpredictable stress-induced depressive rats, which at least partly contributes to the etiology of depression. PMID: 24495952
    3. An increased expression of TPH1 in the dorsal raphe nucleus may be the primary mechanism to explain the chronic increase in the brain synthesis of 5-HT in these in utero malnourished rats. PMID: 22819790
    4. Transfection of VMAT-1 and TPH-1 induced vesicular storage of serotonin which is triggered release upon cooling and has protective effects against hypothermia. PMID: 22253933
    5. An adjacent GC-rich cis -acting sequence cooperates with the inverted CCAAT box for full activation of basal transcription; both elements are essential for full cAMP response. In pinealocytes, NF-Y and Sp1 bind the inverted CCAAT box and GC-rich-region PMID: 12065627
    6. regulation of rat tryptophan hydroxylase gene expression by stress involves 5' untranslated region PMID: 12153488
    7. TPH1 mRNA expression was 105-fold more abundant in rat pineal than TPH2; TPH2 expression within the gland showed no significant variation with time of day and was very low. PMID: 12911638
    8. This study reveals adrenal glucocorticoid-independent induction of tryptophan hydroxylase gene expression in raphe nuclei in response to immobilization stress. PMID: 14744469
    9. TPH2 mRNA is expressed in raphe, in a pattern overlapping the histologically defined raphe nuclei. In sharp contrast, TPH1 (the previously known TPH) is expressed predominantly in pineal gland. PMID: 14960297
    10. Tph1 and Tph2 transcripts showed robust diurnal rhythms of abundance, with highest levels at night. PMID: 15194882
    11. Results show that the synthesis of 5-hydroxytryptamine and the expression of tryptophan hydroxylase in the dorsal raphe of rat offspring were suppressed by maternal ethanol intake. PMID: 16198203
    12. TPH1 mRNA is 2.5-fold upregulated by the stress in dorsal raphe nucleus but not in pineal gland. PMID: 18197473
    13. Data showed that calcium influx blockaded by nifedipine caused a decrease in tryptophan hydroxylase activity. PMID: 18221757
    14. mRNA for TPH-1 was present in peripheral arteries, with low levels of TPH protein and TPH activity PMID: 18414394
    15. data support the model that increased nocturnal 5-HT synthesis in the pineal gland is mediated by the phosphorylation of TPH1 at the serine 58, which elevates the TPH1 protein content and activity at night PMID: 18705647

    显示更多

    收起更多

  • 蛋白家族：
    Biopterin-dependent aromatic amino acid hydroxylase family
  • 数据库链接：

    KEGG: rno:24848

    STRING: 10116.ENSRNOP00000052959

    UniGene: Rn.94788