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  4. Recombinant Rat Steroid 17-alpha-hydroxylase/17,20 lyase (Cyp17a1)

Recombinant Rat Steroid 17-alpha-hydroxylase/17,20 lyase (Cyp17a1)

  • 货号：
    CSB-YP006392RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006392RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006392RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006392RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cyp17a1
  • Uniprot No.：
  • 别名：
    Cyp17a1; Cyp17Steroid 17-alpha-hydroxylase/17,20 lyase; EC 1.14.14.19; 17-alpha-hydroxyprogesterone aldolase; EC 1.14.14.32; CYPXVII; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; Steroid 17-alpha-monooxygenase
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-507
  • 氨基酸序列
    MWELVGLLLL ILAYFFWVKS KTPGAKLPRS LPSLPLVGSL PFLPRRGHMH VNFFKLQEKY GPIYSLRLGT TTTVIIGHYQ LAREVLIKKG KEFSGRPQMV TQSLLSDQGK GVAFADAGSS WHLHRKLVFS TFSLFKDGQK LEKLICQEAK SLCDMMLAHD KESIDLSTPI FMSVTNIICA ICFNISYEKN DPKLTAIKTF TEGIVDATGD RNLVDIFPWL TIFPNKGLEV IKGYAKVRNE VLTGIFEKCR EKFDSQSISS LTDILIQAKM NSDNNNSCEG RDPDVFSDRH ILATVGDIFG AGIETTTTVL KWILAFLVHN PEVKKKIQKE IDQYVGFSRT PTFNDRSHLL MLEATIREVL RIRPVAPMLI PHKANVDSSI GEFTVPKDTH VVVNLWALHH DENEWDQPDQ FMPERFLDPT GSHLITPTQS YLPFGAGPRS CIGEALARQE LFVFTALLLQ RFDLDVSDDK QLPRLEGDPK VVFLIDPFKV KITVRQAWMD AQAEVST
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase involved in corticoid and androgen biosynthesis. Catalyzes 17-alpha hydroxylation of C21 steroids, which is common for both pathways. A second oxidative step, required only for androgen synthesis, involves an acyl-carbon cleavage. The 17-alpha hydroxy intermediates, as part of adrenal glucocorticoids biosynthesis pathway, are precursors of cortisol. Hydroxylates steroid hormones, pregnenolone and progesterone to form 17-alpha hydroxy metabolites, followed by the cleavage of the C17-C20 bond to form C19 steroids, dehydroepiandrosterone (DHEA) and androstenedione. Has 16-alpha hydroxylase activity. Catalyzes 16-alpha hydroxylation of 17-alpha hydroxy pregnenolone, followed by the cleavage of the C17-C20 bond to form 16-alpha-hydroxy DHEA. Also 16-alpha hydroxylates androgens, relevant for estriol synthesis. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
  • 基因功能参考文献：
    1. Data suggest that CYP17A1 and aromatase/Cyp19a1 are induced in hippocampus by the environmental pollutant/retinoid X receptor agonist tributyltin; thus, tributyltin appears to stimulate estradiol biosynthesis. PMID: 24679120
    2. Data from a rat model of polycystic ovary syndrome suggest that hyperandrogenism develops and this may partially up-regulate expression of steroidogenic enzymes (CYP17 and P450scc [cholesterol side-chain cleavage enzyme]) in ovarian tissue. PMID: 22798247
    3. these data demonstrate the occurrence of P450C(17) in the deep cerebellar nuclei of the rat PMID: 22800812
    4. Data demonstrate, for the first time, the presence and anatomical distribution of P450C(17) in the vestibular nuclei. PMID: 21388459
    5. Dibromoacetic acid can directly target rat Leydig cells and downregulate testicular CYP17 expression with a resulting decreased testicular testosterone production. PMID: 21046364
    6. immunolocalization of 3beta-hydroxysteroid dehydrogense, cytochrome P450c17 and androgen receptor were investigated in rat cumuli oophori of late pre-ovulatory follicles and in post-ovulatory COCs bearing fertilized oocytes PMID: 19775733
    7. Results suggest that PCB126 may affect testicular steroidogenesis by downregulating P450(17alpha), P450(scc) and have inhibitory effect on the testicular functions. PMID: 20356859
    8. 17beta-estradiol is synthesized by P45017alpha and P450 aromatase localized in hippocampal neurons from endogenous cholesterol PMID: 14694190
    9. CYP17 is expressed in rat kidney throughout the life-span and has a role in autonomous hormonal steroidogenesis PMID: 15261307
    10. Cyp17a1 in beta-cells of rat pancreas and its local steroidogenesis are reported. PMID: 18556192
    11. GDF-9 plays an important role in promoting preantral follicle growth by up-regulating follicular androgen biosynthesis. GDF-9 is essential for CYP17A1 expression during follicular development from the preantral to the early antral stage. PMID: 19213837
    12. The expression of the mouse CYP17A1 gene is epigenetically controlled. PMID: 19403566

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  • 亚细胞定位：
    Endoplasmic reticulum membrane. Microsome membrane.
  • 蛋白家族：
    Cytochrome P450 family
  • 数据库链接：

    KEGG: rno:25146

    STRING: 10116.ENSRNOP00000027160

    UniGene: Rn.10172