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  4. Recombinant Rat Protein phosphatase 1 regulatory subunit 1B (Ppp1r1b)

Recombinant Rat Protein phosphatase 1 regulatory subunit 1B (Ppp1r1b)

  • 货号：
    CSB-YP757122RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP757122RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP757122RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP757122RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP757122RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ppp1r1b; Protein phosphatase 1 regulatory subunit 1B; DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-205
  • 氨基酸序列
    MDPKDRKKIQ FSVPAPPSQL DPRQVEMIRR RRPTPALLFR VSEHSSPEEE SSPHQRTSGE GHHPKSKRPN PCAYTPPSLK AVQRIAESHL QTISNLSENQ ASEEEDELGE LRELGYPQED DEEDEDEDEE EDEEEDSQAE VLKGSRGTAG QKLTSGQGLE GPWERPPPLD EPQRDGNSED QGEGRATQSE PGEEPRHPTP PESGT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Inhibitor of protein-phosphatase 1.
  • 基因功能参考文献：
    1. Results indicate that the M4 muscarinic receptor plays a critical role in modulating phosphorylation of DARPP-32-Thr75 in striatal medium spiny neurons; suggest that M4 receptor activation acts antagonistically with dopamine D1-like receptors within the striatum, and indicate that M4 receptors may be a potential target for the treatment of Parkinson's disease and other relevant central nervous system disorders. PMID: 28257887
    2. In a model of subarachnoid hemorrhage, rats with hydrocephalus had more severe basal ganglia injury with greater DARPP-32 depletion than rats without hydrocephalus. PMID: 26463938
    3. we also demonstrated frequent co-overexpression and positive correlation of DARPP-32, SRp20 and CD44E expression levels in human gastric primary tumors. PMID: 26119931
    4. Darpp32 interaction with beta-adducin may be the molecular pathway by which environmental changes may rapidly alter responsiveness of striatal neurons involved in the reward system. PMID: 26639316
    5. Study demonstrated that DARPP-32 was not altered in prefrontal cortex and hippocampus in sepsis PMID: 24978930
    6. In cortical & striatal brain regions, 3 different neuronal populations either expressing SSTRs & DARPP-32 alone or displaying colocalization were identified. SSTR-positive neurons lacking DARPP-32 colocalization may be spared in excitotoxicity. PMID: 22116741
    7. DARPP32 has a role in pluripotent stem cell differentiation toward medium-sized spiny neurons PMID: 23250204
    8. This study demonistrated that Changes in nucleus accumbens and neostriatal c-Fos and DARPP-32 immunoreactivity during different stages of food-reinforced instrumental training PMID: 22462413
    9. Fine-tuning of DARPP-32 levels leads to changes in the kinetics or sensitivity of MAPK/ERK signaling. PMID: 22301787
    10. In the striatum of methamphetamine-sensitized rats, phosphothreonine-DARPP-32 immunoreactivities are increased, but DARPP-32 immunoreactivities are not significantly different. PMID: 11954050
    11. acute stress resulted in the activation of DARPP-32 neurons, which were innervated by PACAP-ir neuronal structures in the extended amygdala PMID: 12409216
    12. DARPP-32 phosphorylation was studied in morphine-sensitized rats at different times after morphine challenge which increased levels of phospho-Thr75 DARPP-32 and decreased phospho-Thr34 DARPP-32 levels with time in caudate-putamen and nuucleus accumbens PMID: 15287884
    13. Based on the shape and spatial distribution of these cells, their staining by an anti-parvalbumin antibody, and their juxtaposition with processes containing tyrosine hydroxylase, that DARPP-32 is present in AII amacrine cells of rat retina. PMID: 15515184
    14. Methamphetamine induced an increase in phosphorylation of DARPP-32/Thr75. PMID: 15536496
    15. DARPP-32 is critical for the maintenance of thyroid differentiation by TSH and IGF-I, and loss of DARPP-32 expression may be a characteristic of thyroid cancer. PMID: 16020482
    16. the deletion of KKIQF, a PP1-binding motif of DARPP-32, resulted in a remarkable reduction in its affinity with PP1. PMID: 16428298
    17. This is the first report that DARPP-32 phosphorylation within the prefrontal cortex is associated with recognition as well as temporal order memory for objects. PMID: 16687181
    18. In some cortical structures (prelimbic and cingulate cortices, the dentate gyrus) as well as in the central amygdala, RHA-I rats showed higher DARPP-32 mRNA expression than RLA-I rats PMID: 17008016
    19. These data indicate that DARPP-32 participates in dopamine-mediated modifications of retinal function. PMID: 17552992
    20. These data suggest that a tonic increase in mGluR5 transmission in cocaine-sensitized rats sustains both the increase in phospho-Thr75 DARPP-32 levels and the expression of behavioral sensitization. PMID: 17680995
    21. although DARPP-32 impacts cortical inhibition through a previously demonstrated D2-dependent regulation of GABAergic currents in pyramidal cells, it is not involved in the direct D1-mediated regulation of fast-spiking interneurons PMID: 17693396
    22. Collectively, these findings suggested that changes of the DARPP-32 phosphorylation state may be important for over-activation of the direct pathway. PMID: 17884291
    23. chronic electroconvulsive stimulation increased transient expression of DARPP-32 in several time frames, in striatum and hippocampus PMID: 17919461
    24. results showed that inhibitory avoidance (IA) induced changes on DARPP-32 expression in striatum and hippocampus. DARPP-32 expression changes corroborate with changes in expression and phosphorylation of CREB, NMDA, AMPA after IA PMID: 18415674
    25. The nuclear accumulation of DARPP-32, a potent inhibitor of protein phosphatase-1, increases the phosphorylation of histone H3, an important component of nucleosomal response. PMID: 18496528
    26. upon phosphorylation of Thr(34) in DARPP-32, which turns DARPP-32 into a potent inhibitor of PP1, neither local nor long-range structure of DARPP-32 is altered PMID: 18954090
    27. Basal and cocaine-induced sex differences in the DARPP-32/PP-1 cascade may be responsible for the sexual dimorphism in acute cocaine-induced behavioral responses. PMID: 18985320
    28. Methylphenidate induces changes in total DARPP-32 expression that are posology- and age-related in some rat brain areas, although further studies are needed to shed more light on the mechanisms behind these findings. PMID: 19041388
    29. All five dopamine receptor subtypes and DARPP-32 were expressed in rat brain cortex and striatum. DARPP-32 positive neurons displayed comparative colocalization with dopamine receptor subtypes 1-5. PMID: 19465068
    30. Within the dopamine- and cAMP-regulated phosphoprotein of 32 kDa (DARPP-32) cascade of enzymes that plays a central role in signal integration of dopaminoceptive neurons multiple catalytic and regulatory subunits change their mRNA expression levels PMID: 19474310
    31. in the midbrain ventral tegmental area, progesterone's actions to facilitate sexual behavior of female rodents, involving D1 receptors, may require DARPP-32. PMID: 19782104

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Protein phosphatase inhibitor 1 family
  • 数据库链接：

    KEGG: rno:360616

    STRING: 10116.ENSRNOP00000034614

    UniGene: Rn.70366