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  4. Recombinant Rat Platelet-derived growth factor receptor alpha (Pdgfra), partial

Recombinant Rat Platelet-derived growth factor receptor alpha (Pdgfra), partial

  • 货号：
    CSB-YP017712RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP017712RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP017712RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP017712RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP017712RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pdgfra; Platelet-derived growth factor receptor alpha; PDGF-R-alpha; PDGFR-alpha; EC 2.7.10.1; Alpha platelet-derived growth factor receptor; Alpha-type platelet-derived growth factor receptor; CD140 antigen-like family member A; Platelet-derived growth factor alpha receptor; CD antigen CD140a
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi. Plays a role in cell migration and chemotaxis in wound healing. Plays a role in platelet activation, secretion of agonists from platelet granules, and in thrombin-induced platelet aggregation. Binding of its cognate ligands - homodimeric PDGFA, homodimeric PDGFB, heterodimers formed by PDGFA and PDGFB or homodimeric PDGFC -leads to the activation of several signaling cascades; the response depends on the nature of the bound ligand and is modulated by the formation of heterodimers between PDGFRA and PDGFRB. Phosphorylates PIK3R1, PLCG1, and PTPN11. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, mobilization of cytosolic Ca(2+) and the activation of protein kinase C. Phosphorylates PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and thereby mediates activation of the AKT1 signaling pathway. Mediates activation of HRAS and of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3 and STAT5A and/or STAT5B. Receptor signaling is down-regulated by protein phosphatases that dephosphorylate the receptor and its down-stream effectors, and by rapid internalization of the activated receptor.
  • 基因功能参考文献：
    1. Treatment with different concentrations of PDGF increased the levels of phosphorylated protein kinase B (AKT), beta-catenin, and Cx43, whereas inhibition of PDGFR or activation of phosphatidylinositol 3 kinase (PI3K)/AKT signaling altered b-catenin and Cx43 expression. PMID: 29174980
    2. PDGFs could exert their mechanism of action through an autocrine/paracrine effect on granulosa and theca cells mediated by PDGFRs. PMID: 25937181
    3. IL-1beta-induced MMP-9 expression is mediated through a c-Src-mediated transactivation of PDGFR/PI3K/Akt cascade linking to p47(phox)/NADPH oxidase 2 (Nox2)/reactive oxygen species signaling pathway. PMID: 25159478
    4. PDGF regulates chondrocyte proliferation through activation of ERK1/2 pathway by upregulation of GIT1 expression and Rac1 phosphorylation. PMID: 24420748
    5. During myocardial fibrosis of desoxycorticosterone induced salt-sensitive hypertensive rats, PDGFRalpha acts at early stage, but PDGFRbeta functions in the whole process PMID: 24427322
    6. Endothelin-1 has a role in activating ERK1/2 via transactivation of platelet-derived growth factor receptor in rat L6 myoblasts PMID: 24735959
    7. PDGFRalpha-positive peritubular cells are steroidogenic competent progeny of stem Leydig cells. PMID: 23545158
    8. The results show that beta2-AR transactivates PDGFR thereby promoting survival of cardiomyocytes PMID: 21954875
    9. Angiotensin II can stimulate PDGF receptor expression in vascular smooth muscle cells. PMID: 21171332
    10. Results provide new insight into the regulatory mechanisms of Pdgfra transcription in Leydig cells, which includes a role for the transcription factors SP1 and SP3. PMID: 21289081
    11. Oxidative stress through the activation of c-Src and resultant transactivation of growth factor receptor contributes to the high glucose-induced enhanced expression of Gq/11alpha/PLC and -mediated cell signaling through MAPK/PI3K pathway. PMID: 20036247
    12. EV71-induced COX-2 expression associated with prostaglandin E(2) production is mediated through activation of c-Src/PDGFR/PI3K/Akt/p42/p44 MAPK to initiate the expression of AP-1. PMID: 20333648
    13. Both PDGFalphaR and NG2 are markers of developing OPCs. PDGFalphaR is specific to the NG2+ OPCs and mainly plays an important role at early developmental stages of OPCs. PMID: 19665498
    14. immunohistological studies suggested a role of ERK and PDGFR-alpha in axon regeneration PMID: 19909480
    15. PDGF did not induce proliferation in COX-2+ cells PMID: 11756433
    16. results suggest that delivery of the PDGF-A gene by adenovirus elicits the production of PDGF-AA protein with extended effects on PDGFalphaR downregulation and tyrosine phosphorylation PMID: 11832339
    17. PDGFR-alpha protein and transcriptional regulation in vascular smooth muscle is under the positive regulatory influence of the zinc finger nuclear protein, Sp1 PMID: 14593115
    18. key regulatory role of PDGF in the pathogenesis of cytomegalovirus infection-enhanced obliterative bronchiolitis PMID: 15021824
    19. The presence of PDGF-A is modified in the course of ischemia/reperfusion-induced acute pancreatitis; in pancreatic ducts, the presence of PDGF-A is reduced between the 1st and 3rd, and between the 7th and 9th day of reperfusion. PMID: 15613744
    20. These data show, for the first time, a marked phenotypic shift in expression levels of the PDGF-AA isoform and its receptor tyrosine kinase in the progenitor smooth muscle cells developing in the microvessels of the adult hypertensive lung. PMID: 16794827
    21. PDGF can further increase PDGFR auto-phosphorylation despite strong Src kinase activity, but diffuse downstream activation of PI3-K by Src abrogates cell polarization and chemotaxis: "signalling requires silence". PMID: 17335807
    22. First demonstration of control of differentiation of neural stem/progenitor cells (specifically, oligodendrocytes) in cell-adhesive matrices with immobilized PDGF-AA. PMID: 18801569
    23. PDGFR-[alpha] mRNA Is Upregulated in Acutely-Rejecting, and PDGF-A and PDGF-C mRNA in Chronically-Rejecting Cardiac Allografts PMID: 19213942

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  • 亚细胞定位：
    Cell membrane; Single-pass type I membrane protein. Cell projection, cilium. Golgi apparatus.
  • 蛋白家族：
    Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
  • 数据库链接：

    KEGG: rno:25267

    STRING: 10116.ENSRNOP00000003077

    UniGene: Rn.55127