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  4. Recombinant Rat Early growth response protein 1 (Egr1)

Recombinant Rat Early growth response protein 1 (Egr1)

  • 货号：
    CSB-YP007484RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007484RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007484RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007484RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007484RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Egr1Early growth response protein 1; EGR-1; Nerve growth factor-induced protein A; NGFI-A; Transcription factor Zif268; Zinc finger protein Krox-24
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-508
  • 氨基酸序列
    MDNYPKLEEM MLLSNGAPQF LGAAGTPEGS GGNNSSSSSS SSSGGGGGGG SNSGSSAFNP QGEPSEQPYE HLTTESFSDI ALNNEKALVE TSYPSQTTRL PPITYTGRFS LEPAPNSGNT LWPEPLFSLV SGLVSMTNPP TSSSSAPSPA ASSSSSASQS PPLSCAVPSN DSSPIYSAAP TFPTPNTDIF PEPQSQAFPG SAGTALQYPP PAYPATKGGF QVPMIPDYLF PQQQGDLSLG TPDQKPFQGL ENRTQQPSLT PLSTIKAFAT QSGSQDLKAL NNTYQSQLIK PSRMRKYPNR PSKTPPHERP YACPVESCDR RFSRSDELTR HIRIHTGQKP FQCRICMRNF SRSDHLTTHI RTHTGEKPFA CDICGRKFAR SDERKRHTKI HLRQKDKKAD KSVVASSAAS SLSSYPSPVA TSYPSPATTS FPSPVPTSYS SPGSSTYPSP AHSGFPSPSV ATTYASVPPA FPAQVSTFQS AGVSNSFSTS TGLSDMTATF SPRTIEIC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3'(EGR-site) in the promoter region of target genes. Binds double-stranded target DNA, irrespective of the cytosine methylation status. Regulates the transcription of numerous target genes, and thereby plays an important role in regulating the response to growth factors, DNA damage, and ischemia. Plays a role in the regulation of cell survival, proliferation and cell death. Activates expression of p53/TP53 and TGFB1, and thereby helps prevent tumor formation. Required for normal progress through mitosis and normal proliferation of hepatocytes after partial hepatectomy. Mediates responses to ischemia and hypoxia; regulates the expression of proteins such as IL1B and CXCL2 that are involved in inflammatory processes and development of tissue damage after ischemia. Regulates biosynthesis of luteinizing hormone (LHB) in the pituitary. Regulates the amplitude of the expression rhythms of clock genes: ARNTL/BMAL1, PER2 and NR1D1 in the liver via the activation of PER1 (clock repressor) transcription. Regulates the rhythmic expression of core-clock gene ARNTL/BMAL1 in the suprachiasmatic nucleus (SCN).
  • 基因功能参考文献：
    1. Study found an association between contextual fear conditioning deficits in alcohol-exposed animals with a decrease in Egr-1 mRNA expression in the infralimbic and prelimbic subregions of the medial frontal cortex (mPFC); conclude that memory processes in mPFC associated with the context-shock association phase are the most susceptible to the teratogenic effects of neonatal alcohol. PMID: 30346189
    2. Egr-1 plays an important role in TGF-beta1-induced epithelial-to-mesenchymal transition (EMT)of renal tubular epithelial cells and metformin improves EMT while inhibiting Egr-1, which provides a potential novel target to combat renal fibrosis PMID: 30050950
    3. high glucose alters tendon homeostasis through downregulation of the AMPK/Egr1 pathway and the expression of downstream tendon-related genes in tenocytes. PMID: 28266660
    4. The expression of egr1 was rhythmic in the prefrontal cortex and dorsomedial hypothalamus and food reward diminished this rhythm. PMID: 29355377
    5. Study speculates that during infant learning, specific early growth response protein 1 expressing cellular ensembles are "tagged" representing long-term memory formation, and that these cell ensembles may be reactivated during adult learning. PMID: 28391399
    6. Highly expressed Egr-1 may be involved in the recruitment of RNA POL II in GDNF promoter II in a non-binding manner, and thereby involved in regulating GDNF transcription in high-grade glioma cells. PMID: 28187447
    7. Forced swim stress (FS) prior to the complete Freund's adjuvant (CFA) injection enhanced the CFA-induced mechanical hypersensitivity and attenuated the increase in the expression of pCREB and Egr1 in the insular cortex (IC) and anterior cingulate cortex (ACC) of Sprague-Dawley rats. These findings suggested an FS-induced alteration of neuroplasticity in the IC and ACC following CFA injection. PMID: 28866293
    8. This study demonstrating fear conditioning robustly and consistently induces Egr-1 expression in the lateral nucleus of the amygdala,hippocampus and medial prefrontal cortex. PMID: 28814471
    9. PKA-mediated suppression of Ang-II-induced Egr-1 expression and phosphorylation of ERK may be among the mechanisms by which cAMP exerts its vasculoprotective effects PMID: 28460186
    10. Egr-1 (and TGF-Beta1) are up-regulated by sustained hyperosmolarity in the renal medulla. PMID: 28673338
    11. Results show an age-related reduction in the transcription of Egr1 in the dentate gyrus following spatial behavior, whereas in the area CA1, Egr1 is reduced at rest, but its transcription can be effectively driven by spatial behavior to levels equivalent to those observed in adult animals. PMID: 26972614
    12. Results demonstrate that Zif268 functions within the medial prefrontal cortex (mPFC) to influence working memory, long-term memory, and social interaction time. Reinstitution of mPFC Zif268, using a novel adeno-associated-viral construct, abrogated gonadectomy (GNX)-induced working memory and long-term memory impairments, and reductions in social interaction time, but not GNX-induced depression-like behaviors. PMID: 27816701
    13. Data suggest that TGFB2 (the most abundant growth factor in human milk) binding to Tgfb2r elicits robust/rapid response in small intestinal mucosal cells leading to stimulation of Egr1 transport to nucleus and cell differentiation; more than 15 Wnt signaling pathway genes have Egr1 binding sites/response elements; Egr1 binds to Axin1 promoter and functionally activates gene expression. (Axin1 = axis inhibition protein 1) PMID: 27697743
    14. Ang-II-induced Egr-1 expression is mediated by STIM-1/Orai-1/Ca(2+) -dependent signaling pathways in vascular smooth muscle cells. PMID: 28105751
    15. sublytic C5b-9-induced activation of Egr-1/p300-ATF3/Gadd45 axis plays a critical role in glomerular mesangial cells apoptosis in Thy-1N rats. PMID: 27190312
    16. the loss of GCN5 activity and the induction of Egr-1, E2F1 and Bim are involved in the early brain injury (EBI) following subarachnoid haemorrhage (SAH) in rats. PMID: 28125090
    17. Egr1 contributes to the transcriptional program associated with neuronal differentiation. PMID: 28076410
    18. Mammillothalamic tract lesions in rats reduced activity in the retrosplenial cortex, as measured by expression levels of the immediate-early gene zif268 and the metabolic marker cytochrome oxidase; no significant changes were found in the dorsal hippocampus PMID: 27233617
    19. Results show that miR-191 negatively regulates Egr-1 and controls neointima formation after vascular injury. PMID: 27057945
    20. data indicate that Egr-1 promotes Abeta synthesis via transcriptional activation of BACE-1 and suggest that Egr-1 plays role in activation of BACE-1 and acceleration of Abeta synthesis in AD brain. PMID: 27576688
    21. Forced swimming results in reduced DNA methylation at specific 5'-cytosine-phosphate-guanine-3' (CpG)s within c-Fos and Egr-1 gene promoters and untranslated regions in DG neurons. SAM significantly increases DNA methylation at these c-Fos and Egr-1 gene loci and inhibits c-Fos and Egr-1 induction. PMID: 27078100
    22. This study indicated differential Egr-1 activation during development and adulthood in variants of fear conditioning paradigms. PMID: 26778782
    23. In male rats pre-exposed to prenatal stress, observed a "protective" effect of prenatal stress on postnatal stress-induced downregulation of Egr1 expression, which may indicate that prenatal stress exposure may induce "resilience". PMID: 25239865
    24. Egr1 contributes to diabetic nephropathy by enhancing mesangial cell proliferation and extracellular matrix production, in part by interacting with TGF-beta1. PMID: 26279418
    25. Within the amygdala, zif268 expression participates in the extinction of conditioned fear. PMID: 26282370
    26. The targeted knockdown of Zif268 in the brain results in a non-recoverable retrograde amnesia. PMID: 26238574
    27. There is competitive interaction between redox-sensitive transcription factors Egr1 and Sp1 in the early phases of gastric ulcer development. PMID: 26547966
    28. Orchestrated Egr1 and Pitx1 activities depending on specific central and peripheral regulatory inputs could be responsible for physiologically variable Lhb gene promoter activation in vivo. PMID: 25258388
    29. Results showed that the expression of Arc, but not zif268, was significantly increased in the cerebellum following motor skill learning PMID: 25249385
    30. Egr-1 governs pulmonary vascular remodelling and the development of characteristic vascular neointimal lesions PMID: 25028387
    31. Egr1 gene is actively and selectively transcribed in a major sub-population of male LHB+ cells indicates a largely conserved role in gonadotroph function and has provided a basis for further defining this role. PMID: 25139489
    32. Egr-1 mRNA expression has a complex relationship to fear learning in different brain regions and variants of context conditioning. PMID: 24976583
    33. Inhibition of Egr1 expression underlies the anti-mitogenic effects of cAMP in vascular smooth muscle cells. PMID: 24534707
    34. The hyperacetylation of H3K9 at Egr-1 binding sites in promoter region II of the gdnf gene can up-regulate the binding of Egr-1 to increase gdnf gene transcription in glioma cells. PMID: 25201174
    35. Overall, the acute stress and partner familiarity produced a similar pattern of results in adolescents and adults, with both age groups sensitive to the social context. PMID: 24613747
    36. The results suggest that EGR-1 seems to play a critical role in myocardial reperfusion injury. PMID: 24365880
    37. Decreased expression of Arc and Egr-1 might be involved with instability of synaptic plasticity in tinnitus. PMID: 24704997
    38. litter composition modified sex effects on methylation of Egr1 in nucleus accumbens PMID: 23460384
    39. Zif268 in the dorsal horn of the spinal cord is an important molecular correlate of endocannabinoid-mediated Fear-conditioned analgesia PMID: 23873727
    40. The findings suggest that increased expression of Egr-1 within the prefrontal cortex is associated with contextual fear conditioning in the context pre-exposure facilitation effect paradigm. PMID: 23973447
    41. Deep brain stimulation of mediodorsal thalamic nucleus produces robust increases in the expression of zif-268, but not c-fos in the frontal cortex. PMID: 23660497
    42. number and pattern of granule cell expression of zif268 during rest is preserved in aged animals, despite a significant decline in exploration-related zif268 expression PMID: 24028087
    43. These studies establish a new role for Egr-1 in regulating the expression of the mitogenic epidermal growth factor receptor PMID: 23763269
    44. Our results demonstrate that the IEGs, egr1 and arc, may significantly contribute to synaptic reorganization induced by the kindling of seizures PMID: 23744421
    45. Interactions between elevated peritoneal fluid TNF-alpha and overexpression of follicular Egr1 expression may affect downstream protease pathways impeding ovulation in endometriosis. PMID: 23427178
    46. Apelin-13 induced the proliferation and migration of vascular smooth muscle cells as well as the upregulation of osteopontin via the upregulation of Egr-1. PMID: 23973488
    47. Up-regulation of EGR-1 and NAG-1, along with higher reactive oxygen species load may positively regulate the intrinsic pathway of apoptosis for the NSAID mediated chemoprevention of colorectal cancer. PMID: 23435960
    48. The results indicate a key role for heightened methylation of the Egr-1 binding site in hypoxia-mediated programming of PKCepsilon gene repression PMID: 23427086
    49. Egr-1 plays an important role in regulating the HO-1 production induced by ischemia-reperfusion (IR). Downregulation of Egr-1 expression might be one of the mechanisms whereby ischemic postconditioning confers protection against pulmonary IR insult. PMID: 22878149
    50. expression of Egr-1 is up-regulated and temporally distributed in certain cell types after trauma to skeletal muscles. PMID: 22918836

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    EGR C2H2-type zinc-finger protein family
  • 组织特异性：
    Detected in kidney thick ascending limbs and collecting ducts (at protein level).
  • 数据库链接：

    KEGG: rno:24330

    STRING: 10116.ENSRNOP00000026303

    UniGene: Rn.9096