Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Cystathionine gamma-lyase (Cth)

Recombinant Rat Cystathionine gamma-lyase (Cth)

  • 货号：
    CSB-YP006160RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006160RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006160RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006160RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006160RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cth
  • Uniprot No.：
  • 别名：
    Cth; Cystathionine gamma-lyase; EC 4.4.1.1; Cysteine-protein sulfhydrase; Gamma-cystathionase; Probasin-related antigen; PRB-RA
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-398
  • 氨基酸序列
    MQKDASSSGF LPSFQHFATQ AIHVGPEPEQ WSSRAVVLPI SLATTFKQDS PGQSSGFVYS RSGNPTRNCL EKAVAALDGA KHCLTFARGL AATTTITHLL KAGDEVICMD EVYGGTNRYF RRVASEFGLK ISFVDCSKTK LLEAAITPQT KLVWIETPTN PTLKLADIKA CAQIVHKHKD IILVVDNTFM SAYFQRPLAL GADICMCSAT KYMNGHSDVV MGLVSVTSDD LNERLRFLQN SLGAVPSPFD CYLCCRGLKT LQIRMEKHFR NGMAVARFLE SNPRVEKVIY PGLPSHPQHE LAKRQCTGCP GMVSFYIKGT LQHAQVFLKN IKLFALAESL GGYESLAELP AIMTHASVPE KDRATLGISD TLIRLSVGLE DEKDLLEDLG QALKAAHP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the last step in the trans-sulfuration pathway from methionine to cysteine. Has broad substrate specificity. Converts cystathionine to cysteine, ammonia and 2-oxobutanoate. Converts two cysteine molecules to lanthionine and hydrogen sulfide. Can also accept homocysteine as substrate. Specificity depends on the levels of the endogenous substrates. Generates the endogenous signaling molecule hydrogen sulfide (H2S), and so contributes to the regulation of blood pressure. Acts as a cysteine-protein sulfhydrase by mediating sulfhydration of target proteins: sulfhydration consists of converting -SH groups into -SSH on specific cysteine residues of target proteins such as GAPDH, PTPN1 and NF-kappa-B subunit RELA, thereby regulating their function.
  • 基因功能参考文献：
    1. indings show that brief and repeated ischemic postconditioning increased the expression of CSE after ischemia/reperfusion in diabetes mellitus PMID: 29880385
    2. Delayed gastric emptying in diabetic rats is due to down-regulation of the hydrogen sulfide biosynthesis enzyme, cystathionine gamma lyase. PMID: 27324218
    3. These findings indicated that nitric oxide mediated the stimulatory effect of gastric acid on hydrogen sulfide release and protein expression of cystathione gamma lyase. PMID: 26319795
    4. Up Regulation of cystathione gamma lyase and Hydrogen Sulphide in the Myocardium Inhibits the Progression of Isoproterenol-Caffeine Induced Left Ventricular Hypertrophy PMID: 26963622
    5. Overexpression of cystathionine gamma-lyase protects mesenchymal stem cells from hypoxia and serum deprivationinduced apoptosis in vitro. PMID: 25901909
    6. demonstrates for the first time that both hyperglycemia and hyperketonemia mediate a reduction in CSE expression and activity, which can contribute to the impaired H2S signaling associated with diabetes PMID: 24610811
    7. Treatment of mesangial cells with platelet-derived growth factor-BB resulted in a marked upregulation of cystathionine-gamma-lyase expression and activity accompanied by increased formation of reactive oxygen species. PMID: 22428706
    8. L-Arginine reverses the down-regulation of CSE/H2S system in high pulmonary blood flow-induced pulmonary hypertension. PMID: 21158086
    9. These findings suggest calcium signaling-dependent cystathionine gamma-lyase expression may be mediated via protein phosphorylation of Sp1 and Elk1 in pancreatic beta-cells. PMID: 22133746
    10. HgCl(2), CH(3)Hg-S-Cys and Cys-S-Hg-S-Cys are potent irreversible inhibitors of rat cystathionine gamma-lyase. PMID: 22093698
    11. Overexpression of rat cystathionine gamma-lyase (CSE) stimulates human vascular smooth muscle cell apoptosis due to an increased endogenous production of H2S. PMID: 16507767
    12. The serum levels of H(2)S and CSE activity were significantly increased in hepatic ischemia reperfusion injury. PMID: 21055229
    13. lack of gamma-cystathionase activity in over 50% of old lenses is due to decreased gene expression and proteolytic degradation of the oxidized enzyme; this results in a high risk for the development of senile cataract PMID: 15683713
    14. Increased hepatic gamma-cystathionase activity in the fetal-to-neonatal transition is due to up-regulation of its gene expression mediated by stress hormones together with the physiological oxidative stress that occurs at birth. PMID: 18184479

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Trans-sulfuration enzymes family
  • 数据库链接：

    KEGG: rno:24962

    STRING: 10116.ENSRNOP00000058914

    UniGene: Rn.3881