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  4. Recombinant Rat Cystathionine beta-synthase (Cbs)

Recombinant Rat Cystathionine beta-synthase (Cbs)

  • 货号：
    CSB-YP004589RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004589RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004589RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004589RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004589RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cbs
  • Uniprot No.：
  • 别名：
    CbsCystathionine beta-synthase; EC 4.2.1.22; Beta-thionase; Hemoprotein H-450; Serine sulfhydrase
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-561
  • 氨基酸序列
    PSGTSQCED GSAGCPQDLE VQPEKGQLEK GASGDKERVW ISPDTPSRCT WQLGRPMADS PHYHTVPTKS PKILPDILRK IGNTPMVRIN RISKNAGLKC ELLAKCEFFN AGGSVKDRIS LRMIEDAERA GTLKPGDTII EPTSGNTGIG LALAAAVKGY RCIIVMPEKM SMEKVDVLRA LGAEIVRTPT NARFDSPESH VGVAWRLKNE IPNSHILDQY RNASNPLAHY DDTAEEILQQ CDGKVDMLVA SAGTGGTITG IARKLKEKCP GCKIIGVDPE GSILAEPEEL NQTEQTAYEV EGIGYDFIPT VLDRAVVDRW FKSNDDDSFA FARMLISQEG LLCGGSSGSA MAVAVKAAQE LKEGQRCVVI LPDSVRNYMS KFLSDKWMLQ KGFMKEELSV KRPWWWHLRV QELSLSAPLT VLPTVTCEHT IAILREKGFD QAPVVNESGA ILGMVTLGNM LSSLLAGKVR PSDEVCKVLY KQFKPIHLTD TLGMLSHILE MDHFALVVHE QIQSRDQAWS GVVGGPTDRN NGVSSKQLMV FGVVTAIDLL NFVAAREQTR K
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine. Also involved in the production of hydrogen sulfide, a gasotransmitter with signaling and cytoprotective effects on neurons.
  • 基因功能参考文献：
    1. These findings indicate that decreased liver Bhmt and Cbs-mediated homocysteine metabolism might have a role in hyperhomocysteinemia in polycystic ovary syndrome and provides further evidence for a potential role of decreased liver function in PCOS. PMID: 29804940
    2. cystathionine-beta-synthase might be a potential target for treatment for chronic visceral hypersensitivity in patients with irritable bowel syndrome. PMID: 29712513
    3. Nitration-mediated deficiency of cystathionine beta-synthase activity accelerates the progression of hyperhomocysteinemia. PMID: 29102635
    4. Abundant expression of CBS, localized in renal tubular epithelial cells, was revealed in human and rat renal tissue, which correlated negatively with the progression of fibrotic disease. PMID: 28750410
    5. of CBS activity by O-(Carboxymethyl) hydroxylamine hemihydrochloride (AOAA) significantly attenuated pain hypersensitivity in lumbar disc herniation rats. PMID: 27905525
    6. These results demonstrated that oxygenglucose deprivation induced endoplasmic reticulum stress through the activation of nNOS/NO/AMPK signaling as a result of CBS/H2S system blockage. PMID: 28656194
    7. The results of this study suggested that neonatal HI brain injury may inflict long-term damage by upregulation of CBS and mTOR signaling. PMID: 27465493
    8. We showed that astrocytes in Stroke-prone spontaneously hypertensive rats vulnerable to oxidative stress may be caused by reduction of H2S through lower expression and activity of CBS PMID: 27904035
    9. These results suggest that the lower mRNA expression of cystathionine beta-synthase in the cerebellum and the smaller size of cerebellum may be related to the depression-like behavior in Wistar Kyoto rats. PMID: 26241765
    10. Results indicate that neurons are essential for maintaining the expression and hydrogen sulfide-producing activity of astrocytic Cystathionine beta-synthase in the rat spinal cord. PMID: 25797494
    11. Activation of TLR4 inflammatory bowel disease upregulates CBS expression, which is mediated by the NF-kappaB signaling pathway, contributing to visceral hypersensitivity. PMID: 26229403
    12. CBS was expressed in the rostral, intermediate, and caudal dorsal motor nucleus of the vagus. PMID: 25773343
    13. Results imply that in acute ischemic conditions, CBS is upregulated and activated by truncation causing an increased production of hydrogen sulfide, which exacerbate the ischemic injuries PMID: 25873304
    14. Together data implied that alleviation of plasma homocysteine level by phytoestrogen alpha-zearalanol might be related to the reduction of cystathionine beta-synthase nitration. PMID: 24783194
    15. endogenous hydrogen sulfide generating enzyme CBS plays an important role in temporomandibular joint inflammation. PMID: 24490955
    16. Hypoxia-inducible factors mediate tissue-specific cystathionine beta-synthase expression, which may augment cerebral vasodilation as an adaptive response to chronic hypoxia. PMID: 24328859
    17. Lon protease, a major degradation enzyme in mitochondrial matrix, recognized and degraded mitochondrial CBS by specifically targeting at the oxygenated heme group of CBS proteins. PMID: 23858469
    18. Upregulation of CBS expression might play an important role in developing visceral hyperalgesia via sensitization of sodium channels in peripheral nociceptors. PMID: 23285261
    19. Epigenetic regulation of CBS expression may contribute to inflammatory hyperalgesia. PMID: 23273102
    20. Upregulation of cystathionine beta-synthetase expression by nuclear factor-kappa B activation contributes to visceral hypersensitivity in adult rats with neonatal maternal deprivation. PMID: 23249427
    21. Lanthionine synthetase C-like protein 1 interacts with and inhibits cystathionine beta-synthase: a target for neuronal antioxidant defense. PMID: 22891245
    22. kidney CBS gene expression during ischemia/reperfusion is mediated via a decrease in Sp1 transcriptional activity PMID: 20392694
    23. expression is regulated by insulin administration in alloxan diabetic rat liver PMID: 12198128
    24. Hyperhomocysteinemia is associated with decreased activity and expression of CBS in renal proximal tubules because of defect of chromosome 13 in Dahl salt-sensitive rats. PMID: 16636197
    25. metabolic acidosis during ischemia and accumulation of NO metabolites during reperfusion contributed, in part, to reduced CBS activity leading to an elevation of renal Hcy levels, which in turn, played a detrimental role in the kidney PMID: 18701635
    26. Ischemia-reperfusion reduces cystathionine-beta-synthase-mediated hydrogen sulfide generation in the kidney. PMID: 19439522
    27. The CpG island of the cystathionine beta-synthase promoter was hypomethylated in B-12-deficient rats, and showed no recovery after methionine addition. PMID: 19776636

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Cysteine synthase/cystathionine beta-synthase family
  • 组织特异性：
    Expressed in liver, kidney and brain. Highly expressed in the hippocamus and cerebellum.
  • 数据库链接：

    KEGG: rno:24250

    STRING: 10116.ENSRNOP00000039968

    UniGene: Rn.87853