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  4. Recombinant Rat Cofilin-1 (Cfl1)

Recombinant Rat Cofilin-1 (Cfl1)

  • 货号：
    CSB-YP005280RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005280RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005280RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005280RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005280RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cfl1Cofilin-1; Cofilin; non-muscle isoform
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-166
  • 氨基酸序列
    ASGVAVSDG VIKVFNDMKV RKSSTPEEVK KRKKAVLFCL SEDKKNIILE EGKEILVGDV GQTVDDPYTT FVKMLPDKDC RYALYDATYE TKESKKEDLV FIFWAPESAP LKSKMIYASS KDAIKKKLTG IKHELQANCY EEVKDRCTLA EKLGGSAVIS LEGKPL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Important for normal progress through mitosis and normal cytokinesis. In conjunction with the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions via regulation of actin dynamics. Required for the centralization of the mitotic spindle and symmetric division of zygotes. Plays a role in the regulation of cell morphology and cytoskeletal organization in epithelial cells. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.
  • 基因功能参考文献：
    1. CMIP inhibits cofilin1 activity and alters podocyte cytoskeleton organization at least through RhoA signaling in PAinduced podocyte injury PMID: 28791377
    2. cofilin1 is involved in hypertensive nephropathy by modulating the nuclear translocation of NF-kappaB and the expression of its downstream inflammatory factors in renal tubular epithelial cells PMID: 26450610
    3. Cofilin1 regulates neocortical functional plasticity through the remodeling of dendritic spines within circuits that connect columns. PMID: 25723479
    4. Data indicate that RNAi-mediated silencing of cofilin induces dendritic spine shrinkage. PMID: 24740405
    5. Prevention of the hypertrophic response to adenosine receptor agonists by adenosine receptor activation is mediated by prevention of RhoA stimulation and actin polymerization. PMID: 24096734
    6. Although ADF and cofilin-1 have many redundant functions, each of these proteins has functional differences that affect F-actin structures, cell adhesion and lamellipodial dynamics, all of which are important determinants of cell migration. PMID: 24093776
    7. For the first time, the dynamic cofilin rod formation pattern is demonstrated in detail under stress stimulation by time lapse imaging in cultured neurons. PMID: 22981401
    8. Active cofilin (unphosphorylated form) and inactive cofilin (phosphorylated form) are colocalized at the sexually dimorphic nucleus in the preoptic area, suggesting that actin dynamics occurs in the area. PMID: 23537649
    9. Data indicate that PKCalpha phosphorylates cofilin at Ser-23 and/or Ser-24 during degranulation. PMID: 22855535
    10. This study identifies cofilin1 and its upstream regulators, LIMK and SSH1, as end targets of a NRG1 signaling pathway PMID: 22496574
    11. PGE(2)-dependent activation of cofilin-1 was mediated by the protein phosphatase activity of PTEN, with which it directly associated PMID: 22317922
    12. Cofilin-mediated Bax translocation is a key event in excitotoxic neuronal death as knock down of cofilin has a marked neuroprotective effect on NMDA-mediated neuronal death. PMID: 22117609
    13. Linkage of estrogen communication to cofilin via LIM kinase provides functionality to age-sensitive pLIMK immunoreactivity that has been observed in vivo. PMID: 20696146
    14. It is not yet clear how citron-K signals to cofilin, though it may involve signaling through ROCK, LIM kinase or by directly modulating cofilin. PMID: 20971191
    15. Leukotrienes target F-actin/cofilin-1 to enhance alveolar macrophage anti-fungal activity. PMID: 21715328
    16. RhoC activity is spatially confined to areas surrounding invadopodia; it is controlled by "spatially distinct regulatory elements" that confine RhoC activation within this compartment. RhoC activation enhances cofilin phosphorylation outside invadopodia. PMID: 21474314
    17. Slit-2 triggered the activation of cofilin and the inhibition of RhoA, leading to the collapse of the leading front of olfactory ensheathing cells migration. PMID: 21187345
    18. Slingshot plays a pivotal role in AMPA receptor trafficking and synaptic transmission by controlling actin cytoskeleton via cofilin activation PMID: 20442266
    19. There is a role for cofilin-actin rods in stress-induced disruption of cargo transport and synaptic function within hippocampal neurons. PMID: 19542631
    20. the presence of actin in the nucleus may be required for certain stress-induced responses and cofilin is essential for the nuclear import of actin PMID: 12566455
    21. non-muscle cofilin antibody exclusively labeled tubulobulbar complexes in testis PMID: 14627549
    22. Early phospholipase C and cofilin-dependent barbed end transient is required for the initiation of protrusions and is involved in setting the direction of cell movement in response to EGF. PMID: 15337778
    23. cofilin interacts with S18 protein at the actin-binding site PMID: 15532723
    24. Stimulation with epidermal growth factor (EGF) induces an increase in cofilin activity together with an increase in phosphorylated cofilin (p-cofilin), indicating that cofilin dephosphorylation is not coupled to cofilin activation in these cells. PMID: 16803871
    25. Spatial and temporal control of cofilin activity is required for directional sensing during chemotaxis. PMID: 17113383
    26. These results indicate that Ca(2+) influx through P2X receptors induces the formation of cofilin rods via calcineurin-dependent dephosphorylation of cofilin PMID: 17993279
    27. Coronin 2A and cofilin regulate a subset of focal-adhesion-turnover events. PMID: 19654210

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  • 亚细胞定位：
    Nucleus matrix. Cytoplasm, cytoskeleton. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, lamellipodium.
  • 蛋白家族：
    Actin-binding proteins ADF family
  • 数据库链接：

    KEGG: rno:29271

    STRING: 10116.ENSRNOP00000059624

    UniGene: Rn.11675