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  4. Recombinant Rat Adapter molecule crk (Crk)

Recombinant Rat Adapter molecule crk (Crk)

  • 中文名称：
    Recombinant Rat Adapter molecule crk(Crk),Yeast
  • 货号：
    CSB-YP723607RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat Adapter molecule crk(Crk),Yeast
  • 货号：
    CSB-EP723607RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat Adapter molecule crk(Crk),Yeast
  • 货号：
    CSB-EP723607RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat Adapter molecule crk(Crk),Yeast
  • 货号：
    CSB-BP723607RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat Adapter molecule crk(Crk),Yeast
  • 货号：
    CSB-MP723607RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Crk; CrkoAdapter molecule crk; Proto-oncogene c-Crk; p38
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-304
  • 氨基酸序列
    AGNFDSEER SSWYWGRLSR QEAVALLQGQ RHGVFLVRDS STSPGDYVLS VSENSRVSHY IINSSGPRPP VPPSPAQPPP GVSPSRLRIG DQEFDSLPAL LEFYKIHYLD TTTLIEPVSR SRQGSGVILR QEEAEYVRAL FDFNGNDEED LPFKKGDILR IRDKPEEQWW NAEDSEGKRG MIPVPYVEKY RPASASVSAL IGGNQEGSHP QPLGGPEPGP YAQPSVNTPL PNLQNGPIYA RVIQKRVPNA YDKTALALEV GELVKVTKIN VSGQWEGECN GKRGHFPFTH VRLLDQQNPE EDFS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in cell branching and adhesion mediated by BCAR1-CRK-RAPGEF1 signaling and activation of RAP1.; Regulates cell adhesion, spreading and migration. Mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling.
  • 基因功能参考文献：
    1. overexpression of miR-126 significantly inhibited the activation and migration of hepatic stellate cell through targeting CRK. PMID: 24480980
    2. The Y221 site in transfected rat CrkII regulates Rac membrane translocation upon cell adhesion, which is necessary for activation of downstream Rac signaling pathways. PMID: 12198159
    3. nitric oxide induced dissociation of the Cas-Crk complex was mimicked by overexpression of PTP-PEST in vascular smooth muscle PMID: 12714323
    4. CCK-induced CrkII electrophoretic shift was dependent on the Src family of tyrosine kinases and occurred in the intact animal, suggesting a physiological role of CrkII mediating CCK actions in the exocrine pancreas in vivo PMID: 14622258
    5. Rac1 activation by paxillin-Crk-DOCK180 signaling complex is antagonized by Rap1 in migrating rat cells PMID: 15308668
    6. Crk may be involved in regulation of cell motility by a hyaluronic acid-dependent mechanism through an association with ERMs PMID: 15326184
    7. ROS affect the key adapter protein CrkII signaling by two ways: stimulation of CkII phosphorylation and a functional consequence: formation of CrkII-protein complexes. PMID: 16187300
    8. study provides evidence that Crk phosphorylation induced specifically by engagement of immunoreceptor tyrosine-based inhibition motif-containing receptors contributes to inhibition of NK cells PMID: 18835194

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  • 亚细胞定位：
    Cytoplasm. Cell membrane. Note=Translocated to the plasma membrane upon cell adhesion.
  • 蛋白家族：
    CRK family
  • 组织特异性：
    CRK-II is expressed in all tissues and cells whereas CRK-I is expressed at lower level and in limited cell-types.
  • 数据库链接：

    KEGG: rno:54245

    STRING: 10116.ENSRNOP00000006407

    UniGene: Rn.96136