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  4. Recombinant Pseudomonas aeruginosa PA-I galactophilic lectin (lecA)

Recombinant Pseudomonas aeruginosa PA-I galactophilic lectin (lecA)

  • 中文名称：
    铜绿假单胞菌lecA重组蛋白
  • 货号：
    CSB-YP313124EZX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    铜绿假单胞菌lecA重组蛋白
  • 货号：
    CSB-EP313124EZX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    铜绿假单胞菌lecA重组蛋白
  • 货号：
    CSB-BP313124EZX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    铜绿假单胞菌lecA重组蛋白
  • 货号：
    CSB-MP313124EZX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    lecA
  • Uniprot No.：
  • 别名：
    lecA; pa1L; PA2570; PA-I galactophilic lectin; PA-IL; Galactose-binding lectin
  • 种属：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-122
  • 氨基酸序列
    AWKGEVLAN NEAGQVTSII YNPGDVITIV AAGWASYGPT QKWGPQGDRE HPDQGLICHD AFCGALVMKI GNSGTIPVNT GLFRWVAPNN VQGAITLIYN DVPGTYGNNS GSFSVNIGKD QS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    D-galactose specific lectin. Binds in decreasing order of affinity: melibiose, methyl-alpha-D-galactoside, D-galactose, methyl-beta-D-galactoside, N-acetyl-D-galactosamine. Similar to plant lectins in its selective (carbohydrate-specific) hemagglutinating activity.
  • 基因功能参考文献：
    1. The crystal structure of LecA/melibiose complex shows a secondary sugar binding site with glucose bound. PMID: 24123124
    2. Study established the structural features of PA-IL (LecA} in complex with the three disaccharides by docking and molecular dynamics simulations. PMID: 20410292
    3. It is concluded that the carbohydrate recognizing site of the lectin can have a binding requirement of only one saccharide. PMID: 16542817
    4. Results describe the crystal structure of P. aeruginosa lectin I complexed to alphaGal1-3betaGal1-4Glc trisaccharide at 1.9-A resolution and reveal how the second galactose residue makes specific contacts with the protein surface. PMID: 18762193
    5. The results demonstrate that there is a relationship between the LecA and LecB lectins and the pathogenicity of P. aeruginosa. PMID: 19237519

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  • 亚细胞定位：
    Cytoplasm. Periplasm. Cell surface. Note=Low exposure on the cell surface.
  • 蛋白家族：
    LecA/PllA lectin family
  • 数据库链接：

    KEGG: pae:PA2570

    STRING: 208964.PA2570