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  4. Recombinant Mouse Zinc finger protein Helios (Ikzf2)

Recombinant Mouse Zinc finger protein Helios (Ikzf2)

  • 货号：
    CSB-YP011576MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011576MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011576MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011576MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ikzf2
  • Uniprot No.：
  • 别名：
    Ikzf2; Helios; Zfpn1a2; Znfn1a2; Zinc finger protein Helios; Ikaros family zinc finger protein 2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-526
  • 氨基酸序列
    METDAIDGYI TCDNELSPEG EHANMAIDLT SSTPNGQHAS PSHMTSTNSV KLEMQSDEEC DRQPLSREDE IRGHDEGSSL EEPLIESSEV ADNRKVQDLQ GEGGIRLPNG KLKCDVCGMV CIGPNVLMVH KRSHTGERPF HCNQCGASFT QKGNLLRHIK LHSGEKPFKC PFCSYACRRR DALTGHLRTH SVGKPHKCNY CGRSYKQRSS LEEHKERCHN YLQNVSMEAA GQVMSHHVPP MEDCKEQEPI MDNNISLVPF ERPAVIEKLT ANMGKRKSST PQKFVGEKLM RFSYPDIHFD MNLTYEKEAE LMQSHMMDQA INNAITYLGA EALHPLMQHA PSTIAEVAPV ISSAYSQVYH PNRIERPISR ETSDSHENNM DGPISLIRPK SRPQEREASP SNSCLDSTDS ESSHDDRQSY QGNPALNPKR KQSPAYMKED VKALDATKAP KGSLKDIYKV FNGEGEQIRA FKCEHCRVLF LDHVMYTIHM GCHGYRDPLE CNICGYRSQD RYEFSSHIVR GEHTFH
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Associates with Ikaros at centromeric heterochromatin.
  • 基因功能参考文献：
    1. These data provide a genetic basis for the unstable phenotype of Helios-deficient Tregs within the inflammatory environment of tumors and suggest that immune milieu-dependent alterations in gene expression are a central feature of Treg conversion. PMID: 29440380
    2. He is involved in the correct development of a subset of striatopallidal medium spiny neurons. PMID: 28289129
    3. Helios controls certain aspects of Treg-suppressive function, differentiation, and survival. PMID: 26582951
    4. this study reports that impaired expression of the transcription factor Helios by FoxP3+ CD4 and Qa-1-restricted CD8 Tregs results in defective regulatory activity and autoimmunity in mice. PMID: 26472910
    5. data suggest that a subset of DN T cells, identified by the expression of PD-1 and Helios, represent self-reactive cells; results provide an explanation for the origin of DN T cells and introduce CD8 loss as a process associated with self-Ag encounter PMID: 25825451
    6. results demonstrate that Helios is expressed by thymic and peripheral T cells that are being driven to tolerance in response to a genuine autoantigen PMID: 24740292
    7. identify Helios as the first marker known to diverge during positive and negative selection of thymocytes PMID: 23337809
    8. Helios regulates IL-2 production in Tregs by suppressing Il2 gene transcription. Loss of Helios in Tregs breaks their anergic phenotype and results in derepression of the Il2 locus. PMID: 23275607
    9. Helios expression spatially coincides with the early pattern of expression of the mature markers of striatal projection neurons. PMID: 22142223
    10. In tumor-bearing mice, Helios(+) regulatory T cells were relatively over-represented in the tumor-mass. PMID: 22479644
    11. Data found that Helios-negative T cells are enriched for naive T cell phenotypes and vice versa. Moreover, Helios can be induced during T cell activation and proliferation, but regresses in the same cells under resting conditions. PMID: 21918685
    12. down-regulation of NK cell activity by NKp46 was associated with the silencing of the Helios transcription factor in NK cells PMID: 22267813
    13. show that Helios is upregulated in CD4(+)CD25(+) regulatory T cells. PMID: 20226531
    14. silencing of Helios is critical for normal B cell function PMID: 16148093
    15. results demonstrate a vital function for Helios in maintaining normal homeostasis of developing T cells and formally show that non-DNA-binding isoforms of Helios are lymphomagenic if aberrantly expressed within the T-cell lineage PMID: 17110463
    16. Helios is not essential for T cells; its function can be compensated for by other members of the Ikaros family. PMID: 19620299

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Ikaros C2H2-type zinc-finger protein family
  • 组织特异性：
    Restricted to the T-cell lineage. Abundant in thymus, low expression in bone marrow and brain and no detectable expression in spleen, liver, kidney or muscle.
  • 数据库链接：

    KEGG: mmu:22779

    STRING: 10090.ENSMUSP00000027146

    UniGene: Mm.106343