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  4. Recombinant Mouse Sphingomyelin phosphodiesterase 3 (Smpd3)

Recombinant Mouse Sphingomyelin phosphodiesterase 3 (Smpd3)

  • 中文名称：
    小鼠Smpd3重组蛋白
  • 货号：
    CSB-YP888332MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Smpd3重组蛋白
  • 货号：
    CSB-EP888332MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Smpd3重组蛋白
  • 货号：
    CSB-EP888332MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Smpd3重组蛋白
  • 货号：
    CSB-BP888332MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Smpd3重组蛋白
  • 货号：
    CSB-MP888332MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Smpd3
  • Uniprot No.：
  • 别名：
    Smpd3; Sphingomyelin phosphodiesterase 3; EC 3.1.4.12; Neutral sphingomyelinase 2; nSMase-2; nSMase2; Neutral sphingomyelinase II
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-655
  • 氨基酸序列
    MVLYTTPFPN SCLSALHAVS WALIFPCYWL VDRLLASFIP TTYEKRQRAD DPCCLQLFCT VLFTPVYLAL LVAALPFAFL GFIFWSPLQS ARRPYSYSRL EDKNPAGGAA LLSEWKGTGA GKSFCFATAN VCLLPDSLAR LNNVFNTQAR AKEIGQRIRN GAARPQIKIY IDSPTNTSIS AASFSSLVSP QGGDGSRAVP GSIKRTASVE YKGDGGRHPS DEAANGPASG EQADGSLEDS CIVRIGGEEG GRPQEADDPA AGSQARNGAG GTPKGQTPNH NQRDGDSGSL GSPSASRESL VKARAGQDSG GSGEPGANSK LLYKTSVVKK AAARRRRHPD EAFDHEVSAF FPANLDFLCL QEVFDKRAAA KLKEQLHGYF EYILYDVGVY GCHGCCNFKC LNSGLFFASR YPVMDVAYHC YPNGCSFDAL ASKGALFLKV QVGSTPQDQR IVGYIACTHL HAPPEDSAVR CEQLDLLQDW LADFRKSTSS TSTANPEELV VFDVICGDLN FDNCSSDDKL EQQHSLFTRY KDPCRLGPGE EKPWAIGTLL DTNGLYDEDV CTPDNLQKVL ESEEGRREYL AFPTSKSPGA GQKGRKDLLK GNGRRIDYML HAEEGLCPDW KAEVEEFSFI TQLSGLTDHL PVAMRLMVSA GEEEA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Regulates the cell cycle by acting as a growth suppressor in confluent cells. Probably acts as a regulator of postnatal development and participates in bone and dentin mineralization. Binds to anionic phospholipids (APLs) such as phosphatidylserine (PS) and phosphatidic acid (PA) that modulate enzymatic activity and subcellular location. May be involved in IL-1-beta-induced JNK activation in hepatocytes. May act as a mediator in transcriptional regulation of NOS2/iNOS via the NF-kappa-B activation under inflammatory conditions.
  • 基因功能参考文献：
    1. Data suggest that IL-1beta induction of ceramide, cytokine expression, and leukocyte influx are regulated by brain neutral sphingomyelinase II protein (nSMase). PMID: 28377412
    2. The enzymes involved in sphingolipid metabolism are expressed abnormally in B cells from lupus-prone mice. TLR signaling induced the abnormal expression of sphingomyelin phosphodiesterase 3 (SMPD3). TLR signaling also induced the transport of SMPD3 from the Golgi apparatus. Furthermore, the dysfunction of SMPD3 enhanced TLR-induced inflammatory response of B cells and macrophages in turn. PMID: 28889482
    3. SMPD3 in the regulation of the protein vesicular secretory pathway may become a diagnostic target in the etiology of unknown forms of juvenile growth and developmental inhibition. PMID: 27882938
    4. Study provides evidence that Smpd3 expression in both chondrocytes and osteoblasts is required for normal endochondral bone development. PMID: 27325675
    5. these results highlight the role of nSMase2 in apoptosis evoked by nutrient starvation that could contribute to the delayed apoptosis of hypertrophic chondrocytes in the growth plate, and emphasize the antiapoptotic properties of HAS2 PMID: 25555205
    6. nSMase2 activation is required for the development of infection-induced diaphragm calpain activation and muscle weakness. PMID: 26138644
    7. These results suggest that OTC is a potent stimulant of nSMase-2 expression and that there may be unanticipated complications of OTC supplementation. PMID: 25047167
    8. Src and p38 mitogen-activated protein kinase activities are critical for regulating nSMase2 phosphorylation. PMID: 25347576
    9. Smpd3 was identified as a redox-sensitive enzyme, whose basal activity mediated changes in its oligomeric state. PMID: 25287744
    10. Data confirms a crucial pro-survival role for SMPD3 during embryonic development. PMID: 24585429
    11. Smpd3/nSMase2-ceramide-Akt signaling axis negatively regulates BMP-induced chondrocyte maturation. PMID: 24505141
    12. Neutral sphingomyelinase 2 deficiency is associated with lung anomalies similar to emphysema. PMID: 22945695
    13. a requirement for nSMase2-mediated cancer cell exosomal miRNAs in the regulation of metastasis through the induction of angiogenesis in inoculated tumors. PMID: 23439645
    14. Smpd3 expression in odontoblasts is required for tooth mineralization. PMID: 23428435
    15. initial structure/function insights regarding nSMase2 phosphorylation PMID: 22074919
    16. The Col1a1-Smpd3 mice, in which osteoblast-specific expression of Smpd3 corrected the bone abnormalities observed in fro/fro embryos without affecting the cartilage phenotype. PMID: 21788370
    17. interactions between nSMase2 and anionic phospholipids PMID: 21550973
    18. This study identified nSMase2 as an important component for efficient memory formation and underscores the importance of ceramide in regulating synaptic events related to learning and memory. PMID: 20629193
    19. SMase2 is a structural gene for nSMase, acts as a bona fide N-SMase in cells, and has a role in the regulation of cell growth and cell signaling PMID: 12566438
    20. the smpd3-null mouse develops a novel form of dwarfism and delayed puberty as part of a hypothalamus-induced combined pituitary hormone deficiency PMID: 15764706
    21. role of deletion in gene leads to severe osteogenesis and dentinogenesis imperfecta PMID: 16025116
    22. this novel form of combined pituitary hormone deficiency is characterized by the perturbation of the hypothalamus-pituitary growth axis, associated with retarded chondrocyte development and enchondral ossification in the epiphyseal growth plate. PMID: 17591962
    23. in a mouse osteosarcoma model, a somatic homozygous deletion specifically targeting Smpd3 was identified PMID: 18299447
    24. Results suggest that the smpd3 promoter is activated by BMP2 and is directly regulated by the Runx2 transcription factor. PMID: 19250608

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  • 亚细胞定位：
    Golgi apparatus membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Note=May localize to detergent-resistant subdomains of Golgi membranes of hypothalamic neurosecretory neurons (PubMed:15764706).
  • 蛋白家族：
    Neutral sphingomyelinase family
  • 组织特异性：
    Predominantly expressed in brain (at protein level).
  • 数据库链接：

    KEGG: mmu:58994

    STRING: 10090.ENSMUSP00000069255

    UniGene: Mm.23298