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  4. Recombinant Mouse Sequestosome-1 (Sqstm1)

Recombinant Mouse Sequestosome-1 (Sqstm1)

  • 货号：
    CSB-YP723738MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP723738MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP723738MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP723738MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP723738MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Sqstm1; A170; STAP; Sequestosome-1; STONE14; Ubiquitin-binding protein p62
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-442
  • 氨基酸序列
    ASFTVKAYL LGKEEATREI RRFSFCFSPE PEAEAQAAAG PGPCERLLSR VAVLFPTLRP GGFQAHYRDE DGDLVAFSSD EELTMAMSYV KDDIFRIYIK EKKECRREHR PPCAQEAPRN MVHPNVICDG CNGPVVGTRY KCSVCPDYDL CSVCEGKGLH REHSKLIFPN PFGHLSDSFS HSRWLRKLKH GHFGWPGWEM GPPGNWSPRP PRAGDGRPCP TAESASAPPE DPNVNFLKNV GESVAAALSP LGIEVDIDVE HGGKRSRLTP TTPESSSTGT EDKSNTQPSS CSSEVSKPDG AGEGPAQSLT EQMKKIALES VGQPEEQMES GNCSGGDDDW THLSSKEVDP STGELQSLQM PESEGPSSLD PSQEGPTGLK EAALYPHLPP EADPRLIESL SQMLSMGFSD EGGWLTRLLQ TKNYDIGAAL DTIQYSKHPP PL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Autophagy receptor required for selective macroautophagy (aggrephagy). Functions as a bridge between polyubiquitinated cargo and autophagosomes. Interacts directly with both the cargo to become degraded and an autophagy modifier of the MAP1 LC3 family. Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures) and links ALIS to the autophagic machinery. Involved in midbody ring degradation. May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD. May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels. Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport. Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes. Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes.
  • 基因功能参考文献：
    1. A complex of C9ORF72 and p62 uses arginine methylation to eliminate stress granules by autophagy. PMID: 30022074
    2. Atg7(f/f) Tyr-Cre mice, in which autophagy-related 7 (Atg7) is conditionally deleted under the control of the tyrosinase promoter, are a model for accumulations of the autophagy adapter and substrate sequestosome-1/p62 in both neuronal and neuroepithelial cells. PMID: 29550918
    3. These results suggest that overexpression of SQSTM1 in SOD1 (H46R) mice accelerates disease onset by compromising the protein degradation pathways. PMID: 29843805
    4. The studies findings imply that p62 signaling plays a crucial role in suppressing inflammatory cytokine production by globular adiponectin in macrophages. PMID: 28341848
    5. Data, including data from studies in transgenic and knockout mice, suggest that p62/Sqstm1 is not required for normal pancreatic islet organization and beta-cell secretion of insulin. PMID: 28978813
    6. p62 serves to prevent endoplasmic reticulum stress in mouse hypothalamus by maintaining protein folding capacity PMID: 28619261
    7. Endogenous p62 undergoes E2-dependent ubiquitylation during upregulation of Ubiquitin (Ub) homeostasis, a condition termed as Ub(+) stress, that is intrinsic to Ub overexpression, heat shock or prolonged proteasomal inhibition by bortezomib, a chemotherapeutic drug. PMID: 28322253
    8. analysis of soluble SQSTM1 complexes and soluble complexes formed between SQSTM1 oligomers and LC3 using a combination of fluorescence microscopy-based biophysical approaches in living cells PMID: 27442348
    9. ENC1 interacts with the phosphorylated p62 to impair autophagic degradation of mutant huntingtin protein aggregates. PMID: 26637326
    10. this study proved that SYVN1 enhances SERPINA1(E342K)/ATZ degradation through SQSTM1-dependent autophagy and attenuates SERPINA1(E342K)/ATZ cytotoxicity. PMID: 28121484
    11. Data suggest that maternal nutrition modulates expression of autophagy proteins p62/Sqstm1 and Lc3-II/Map1lc3 in liver and hypothalamus of offspring (newborn, suckling, and adult stages); for example, high-fat diet leading to maternal obesity up-regulates p62 and down-regulates Lc3-II in neonatal liver. (p62/Sqstm1 = sequestosome 1; Lc3-II/Map1lc3 = microtubule-associated protein 1-light chain 3) PMID: 27180121
    12. TRIM11 suppresses AIM2 inflammasome by degrading AIM2 via p62-dependent selective autophagy. PMID: 27498865
    13. SQSTM1 dynamically associates with DNA damage foci (DDF) and regulates DNA repair. PMID: 27391408
    14. Thyroid hormone promotes selective autophagy via induction of DAPK2-SQSTM1 cascade, which in turn protects hepatocytes from diethylnitrosamine-induced hepatotoxicity or carcinogenesis. PMID: 27653365
    15. Our findings show how p62 helps maintain intracellular pools of glutathione needed to limit mitochondrial dysfunction in tumor cells with elevated mTORC1 PMID: 28512249
    16. TRIM14 inhibits cGAS degradation mediated by selective autophagy receptor p62 to promote innate immune responses. PMID: 27666593
    17. s found that p62/SQSTM1, a protein upregulated in liver parenchymal cells but downregulated in HCC-associated HSCs, negatively controls hepatic stellate cells activation. PMID: 27728806
    18. SQSTM1 or damaged proteins may be transported from the nose to the brain. These findings strongly implicate widespread protein damage in the etiology of flavorings-related lung disease. PMID: 27643531
    19. Promoting amino acid sensitivity of the mTOR pathway is dependent on p62 accumulation by inhibition of autophagy and this process plays an important role in the hepatic differentiation of stem/progenitor cells. PMID: 27748507
    20. role of p62 in MDB maturation and stability PMID: 27526095
    21. two ALS-linked factors, SQSTM1 and ALS2, have distinct but additive protective roles against mutant SOD1-mediated toxicity by modulating neuronal proteostasis possibly through the autophagy-endolysosomal system. PMID: 27439389
    22. These results demonstrated that p62-dependent mitophagy has an immunosuppressive role to innate immune response and might serve as a potential immunomodulatory target for inflammation-associated diseases. PMID: 27458013
    23. results indicate that autophagy is important for the removal of excess Endoplasmic Reticulum and hepatic CYP enzymes in mouse livers, a process associated with the autophagy receptor protein p62. PMID: 27325701
    24. p62 reduces UVB-induced apoptosis by modulating intrinsic apoptotic signaling through Src phosphorylation. PMID: 27368125
    25. Our results indicate that JEV replication is impaired in p62-deficient MEFs, suggesting that p62 positively regulates JEV replication in host cells. PMID: 27624873
    26. Results demonstrate that p62 facilitates activation of NRF2 and mTORC1 and is essential for hepatocellular carcinomas (HCC) initiation. PMID: 27211490
    27. p62 may be a crucial mediator for the mitochondrial apoptosis pathway in Monosodium Urate crystal-induced inflammation, which is linked to the acute inflammatory response during the early phase of gout. PMID: 27271513
    28. Results show that Nrf2 prevents TM-induced apoptotic cell death and that TM promotes p62-dependent autophagic Keap1 degradation, leading to Nrf2 activation. In addition, we identified that p62 is essential to protect cells against TM-mediated oxidative damage through Nrf2 activation. PMID: 27780373
    29. the molecular basis for the escape of TBC1D25 from autophagic degradation by performing a chimeric analysis between TBC1D25 and SQSTM1/p62, was investigated. PMID: 26902585
    30. p62 are subjected to parkin mediated proteasomal degradation PMID: 26746706
    31. Data show that sequestosome 1 protein p62 deficiency increased atherosclerotic plaque burden in mice with a macrophage-specific ablation of autophagy-related 5 protein ATG5. PMID: 26732762
    32. These results indicate that Sqstm1-GFP(KI/+) mice are a useful tool for analyzing Sqstm1 in living cells and intact animals. PMID: 26483381
    33. p62 is a novel binding partner for ARIP4, and that its binding regulates the cellular protein level of ARIP4 under conditions of metabolic stress. PMID: 26412716
    34. p62 is recruited together with ubiquitin to the T. gondii PVs, following IFN-gamma stimulation, and plays a specific role in vacuolar-antigen-specific CD8+ T cell activation. PMID: 26440898
    35. TRIM21 plays an essential role in p62-regulated redox homeostasis and may be a viable target for treating pathological conditions resulting from oxidative damage. PMID: 26942676
    36. The p62 double conditional knockout mice demonstrated that p62 aggregate formation triggers aberrant superoxide increases by impairing superoxide dismutase functions. PMID: 26929451
    37. PKCzeta/p62 activation stimulated inflammatory cytokine production and enhanced IGF-I-stimulated VSMC proliferation PMID: 26231202
    38. High expression of cytoplasmic p62 is a novel prognostic biomarker of endometrial cancers PMID: 26162509
    39. Findings suggest that p62 and neighbor of the brca1 gene affect the pathogenesis of neurodegenerative diseases through the cooperative modulation of alpha-synuclein aggregation. PMID: 25412696
    40. autophagic clearance is impaired early after traumatic brain injury due to lysosomal dysfunction, and correlates with neuronal cell death PMID: 25484084
    41. we describe basic protocols to measure the levels of endogenous LC3 and p62 by immunoblotting in cultured mammalian cells PMID: 25484342
    42. These results strongly suggest that p62 plays a crucial role in preventing fenofibrate-induced cell death. PMID: 26282199
    43. Data propose that NF-Y is involved in a unique regulation mechanism of ER organization in mature neurons and its disruption causes previously undescribed novel neuropathology accompanying abnormal ubiquitin/p62 accumulation. PMID: 24566496
    44. Our findings would thus suggest that p62 could be a target for the therapeutic control of prion diseases PMID: 24675871
    45. p62 deficiency results in loss of autophagic repression of macrophage contact-dependent activation of osteoblast NF-kappaB signaling. PMID: 25533346
    46. In the steatotic liver, reduced expression of p62/SQSTM1 induced FasL/Fas overexpression and suppressed antioxidant genes, mainly through Nrf-2 inactivation. PMID: 24925527
    47. SQSTM1 is required for proper dynein motility and trafficking along microtubules. PMID: 25015291
    48. TLR4-activation by lipopolysaccharides in hepatocytes induces a p62-mediated, not beclin1-mediated, autophagy-like clearance pathway. PMID: 24683544
    49. the expression level of SQSTM1 in starved cells is determined by at least 3 factors: autophagic degradation, transcriptional upregulation, and availability of lysosomal-derived amino acids PMID: 24394643
    50. It is a selective substrate forautophagy and its functions are for maintaining adequate body weight and blood pressure. PMID: 25675818

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  • 亚细胞定位：
    Cytoplasm, cytosol. Late endosome. Nucleus. Endoplasmic reticulum. Lysosome. Cytoplasmic vesicle, autophagosome. Nucleus, PML body. Cytoplasm, myofibril, sarcomere.
  • 组织特异性：
    Widely expressed.
  • 数据库链接：

    KEGG: mmu:18412

    STRING: 10090.ENSMUSP00000099835

    UniGene: Mm.40828