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  4. Recombinant Mouse Ribonucleoside-diphosphate reductase subunit M2 (Rrm2)

Recombinant Mouse Ribonucleoside-diphosphate reductase subunit M2 (Rrm2)

  • 货号：
    CSB-YP020519MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP020519MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP020519MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP020519MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP020519MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rrm2; Ribonucleoside-diphosphate reductase subunit M2; EC 1.17.4.1; Ribonucleotide reductase small chain; Ribonucleotide reductase small subunit
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-390
  • 氨基酸序列
    MLSVRTPLAT IADQQQLQLS PLKRLTLADK ENTPPTLSST RVLASKAARR IFQDSAELES KAPTNPSVED EPLLRENPRR FVVFPIEYHD IWQMYKKAEA SFWTAEEVDL SKDIQHWEAL KPDERHFISH VLAFFAASDG IVNENLVERF SQEVQVTEAR CFYGFQIAME NIHSEMYSLL IDTYIKDPKE REYLFNAIET MPCVKKKADW ALRWIGDKEA TYGERVVAFA AVEGIFFSGS FASIFWLKKR GLMPGLTFSN ELISRDEGLH CDFACLMFKH LVHKPAEQRV REIITNAVRI EQEFLTEALP VKLIGMNCTL MKQYIEFVAD RLMLELGFNK IFRVENPFDF MENISLEGKT NFFEKRVGEY QRMGVMSNST ENSFTLDADF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides. Inhibits Wnt signaling.
  • 基因功能参考文献：
    1. mice carrying extra alleles of the RNR regulatory subunit RRM2 (Rrm2(TG)) present supraphysiological RNR activity and reduced chromosomal breakage at fragile sites PMID: 25838540
    2. R2 expression may discriminate cervical cancer phenotype and radiochemotherapy outcome PMID: 23552804
    3. Cinobufotalin significantly inhibits the growth of the xenografts of endometrial carcinoma Ishikawa in nude mice by inhibiting RRM2 expression. PMID: 21275172
    4. Data suggest that RRM2 may be an important effector of progesterone signaling to induce cell proliferation and decidualization in uterus. PMID: 22403396
    5. exclusion as candidate congenital polycystic kidney gene PMID: 11701955
    6. illegitimate recombination initiated by c-Myc PMID: 11840336
    7. Adrenergic stimulation of brown adipocytes elevates ribonucleotide reductase subunit R2 in the proliferative stage of adipocyte development; mediating pathways include cAMP/PKA cascades, Src and Erk Map Kinases. PMID: 11900481
    8. Cobalt substitution of mouse R2 ribonucleotide reductase as a model for the reactive diferrous state. PMID: 12087093
    9. The small subunit R2 of murine ribonucleotide reductase (RR) may be important for nuclear RR activity and for RR activity in cells having abnormally low ATP levels, such as cancer cells. PMID: 12578384
    10. S Phase-specific transcription of the mouse ribonucleotide reductase R2 gene requires both a proximal repressive E2F-binding site and an upstream promoter activating region PMID: 14688249
    11. x-ray crystallographic analysis of the native dinuclear iron center of ribonucleotide reductase protein R2 PMID: 15322079
    12. Results, supported by molecular modeling, strongly suggest that Arg265 of ribonucleotide reductase R2 is involved in the proton-coupled electron transfer pathway and may act as a proton mediator during catalysis. PMID: 16829694
    13. Chk1 is required for DNA replication at least through regulating RNR2 gene transcription. PMID: 18616928
    14. results indicate that the affinity of the RNR R2 proteins for binding metals is determined by the nature of one specific residue in the vicinity of the dimetal site, namely the one that carries the tyrosyl radical in class Ia and Ib R2 proteins PMID: 19492792
    15. The structure of the active site of ribonucleotide reductase M2 (PDB 1XSM) in the radical state (tyrosyl radical) was studied using EPR. PMID: 19639135

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Ribonucleoside diphosphate reductase small chain family
  • 数据库链接：

    KEGG: mmu:20135

    STRING: 10090.ENSMUSP00000020980

    UniGene: Mm.471826