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  4. Recombinant Mouse Rho-related GTP-binding protein RhoB (Rhob)

Recombinant Mouse Rho-related GTP-binding protein RhoB (Rhob)

  • 货号：
    CSB-YP019683MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP019683MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP019683MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP019683MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP019683MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Rhob; Arhb; Rho-related GTP-binding protein RhoB
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-193
  • 氨基酸序列
    MAAIRKKLVV VGDGACGKTC LLIVFSKDEF PEVYVPTVFE NYVADIEVDG KQVELALWDT AGQEDYDRLR PLSYPDTDVI LMCFSVDSPD SLENIPEKWV PEVKHFCPNV PIILVANKKD LRSDEHVRTE LARMKQEPVR TDDGRAMAVR IQAYDYLECS AKTKEGVREV FETATRAALQ KRYGSQNGCI NCC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. Serves as a microtubule-dependent signal that is required for the myosin contractile ring formation during cell cycle cytokinesis. Required for genotoxic stress-induced cell death in breast cancer cells.
  • 基因功能参考文献：
    1. These results highlight a newly identified function for RhoB in supporting the specific production of pathogenic autoantibodies. PMID: 28882929
    2. the histone deacetylase inhibitor trichostatin A increased miR-223 expression obviously in TLR-triggered macrophages, which in turn suppressed RhoB expression and downstream IL-6 production, suggesting that the inhibition of miR-223 by histone deacetylation may be involved in the regulation of TLR-activated inflammatory response. PMID: 28881218
    3. these results suggest that RhoB plays an important role in the hypoxia-induced activation of macrophages and the inflammatory response. PMID: 26388235
    4. RhoB-deficient cells accumulate endogenous gammaH2AX and chromosomal abnormalities, suggesting that RhoB loss increases double-strand break-mediated genomic instability and tumor progression. PMID: 24912678
    5. Loss of RhoB decreases pathological angiogenesis in the ischemic retina and reduces angiogenesis in response to cutaneous wounding, but enhances lymphangiogenesis following both dermal wounding and inflammatory challenge PMID: 24280686
    6. UVB-induced RhoB expression participates in in vivo squamous cell carcinoma initiation by increasing keratinocyte survival. PMID: 23792460
    7. the cytoprotective RhoB response is not only evoked by bacterial protein toxins inactivating Rho/Ras proteins but also by the Rac1-activating toxin CNF1. PMID: 23732113
    8. In cancer cells in vitro or in vivo, RhoB functions as a tumor suppressor that restricts EGF receptor (EGFR) cell surface occupancy as well as Akt signaling PMID: 23135917
    9. RhoB mediates adaptational changes to acute hypoxia in the vasculature, but its continual activation by chronic hypoxia can accentuate vascular remodeling to promote development of pulmonary hypertension. PMID: 22539766
    10. These data directly implicate neuronal RhoB in caspase 3 activation and the initial stages of programmed cell death. PMID: 22098422
    11. miR-21 as a new angiogenesis inhibitor through repression of RhoB PMID: 21347332
    12. findings strongly support a physiological role of RhoB in regulation of thymus development and maintenance through the inhibition of TGF-beta signaling in thymic medullary epithelium PMID: 21865151
    13. RhoB links PDGF signaling to cell migration by coordinating activation and localization of Cdc42 and Rac. PMID: 21344485
    14. RhoB is a modifier of diabetes, important for the induction of beta-cell loss. PMID: 21224061
    15. These data implicate RhoB in cofilin regulation and dendritic and spine morphology, highlighting its importance in synaptic plasticity at a structural and functional level. PMID: 20203211
    16. RhoB-regulated mDia1 has a role in the regulation of hematopoietic progenitor cells PMID: 19768111
    17. Results suggest that constitutive activation of the Rho-ROCK-LIM kinase pathway by extracellular production of lysophosphatidic acid by the action of autotaxin is required to maintain the large size of lysosomes in visceral endoderm cells. PMID: 19808661
    18. RhoB is dispensable for normal cell physiology but it has a suppressor or negative modifier function in stress-associated processes including cancer. PMID: 11905808
    19. RhoB isoprenylation is necessary for its rapid turnover; there is a link between the cholesterol biosynthetic pathway and the regulation of G-protein expression PMID: 12384510
    20. The geranylgeranyltransferase-I inhibitor GGTI-298 inhibits the RhoB pathway and disrupts stress fiber and focal adhesion formation in NIH-3T3 cells. PMID: 12548020
    21. RhoB expression is suppressed by EGFR, ErbB2 and Ras, but not Src. PMID: 14647415
    22. RhoB has a role as a tumor suppressor in lung neoplasms PMID: 15102679
    23. suppression of RhoB as a mechanism by which the Ras/PI3K/Akt pathway induces tumor survival, transformation, invasion, and metastasis PMID: 15169915
    24. Ultraviolet light induces stress-activated signal transduction leading to nuclear/cytoplasmic shuttling of HuR proteinand subsequent stabilization of RhoB mRNA. PMID: 15543229
    25. RhoB mediated transcriptional suppression but also accumulation of Cyclin B1 in the cytosol at early times after farnesyl transferase inhibitor treatment, at a time before the subsequent reduction in steady-state protein levels PMID: 15548709
    26. RhoB and actin polymerization coordinate Src activation with endosome-mediated delivery to the membrane PMID: 15572128
    27. RhoB-F and RhoB-GG which differ only by a 5-carbon isoprene behave differently in rodent cells highlighting the important role of prenyl groups in protein function and emphasize the potency of RhoB to regulate negatively the oncogenic signal. PMID: 15748883
    28. Farnesylated Rhob inhibits radiation-induced mitotic cell death and controls radiation-induced centrosome overduplication. PMID: 15776002
    29. The ability of RhoB to support GSK-3-dependent turnover of c-Myc offers a mechanism by which RhoB acts to limit the proliferation of neoplastically transformed cells PMID: 16247449
    30. These data suggest that, although RhoB is expressed by Muller cells in chick and mouse, its role in the maintenance of cellular morphology and regulation may be different. PMID: 16385489
    31. RhoB was upregulated by Rho-/Ras-inactivating toxins, as a consequence of the inactivation of either Rho(A,B,C) or (H-,K-,N-)Ras. In TcdB-treated cells, RhoB escaped its inactivation and was required for the cytotoxic effect. PMID: 17397186
    32. RhoB affects cell shape and migration by regulating surface integrin levels. PMID: 17692842
    33. mouse RhoB transcription is epigenetically regulated in a tissue-specific manner during aging by histone modification, but not by CpG methylation PMID: 17706596
    34. Suggest RhoB is a critical regulator of PDGFR-beta trafficking and signaling in vascular smooth muscle cells. PMID: 17951322
    35. Vibrio parahaemolyticus induces a rapid remodeling of macrophage actin and activates RhoB GTPase via translocation of VP1686. PMID: 18239436
    36. In vivo restoration of RhoB expression leads to ovarian tumor regression. PMID: 18340357
    37. Rhob plays an important role in the antiproliferative effect of glucocorticoid receptor (GR) on RAW264.7 cells. PMID: 18840672
    38. Rhob expression is associated with hematopoietic stem cells that have durable self-renewal potential PMID: 19377048
    39. RhoB is essential for apoptosis and antineoplastic activity of farnesyltransferase inhibitors in a mouse model. PMID: 10913192
    40. Loss of RhoB did not adversely affect mouse development, fertility, or wound healing. However, embryo fibroblasts cultured in vitro exhibited a defect in motility, suggesting that RhoB has a role in this process that is conditional on cell stress PMID: 11564874

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  • 亚细胞定位：
    Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Cleavage furrow.
  • 蛋白家族：
    Small GTPase superfamily, Rho family
  • 数据库链接：

    KEGG: mmu:11852

    STRING: 10090.ENSMUSP00000067013

    UniGene: Mm.687