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  4. Recombinant Mouse Protein kinase C epsilon type (Prkce)

Recombinant Mouse Protein kinase C epsilon type (Prkce)

  • 货号：
    CSB-YP018704MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018704MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018704MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018704MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018704MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Prkce; Pkce; PkceaProtein kinase C epsilon type; EC 2.7.11.13; nPKC-epsilon
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-737
  • 氨基酸序列
    MVVFNGLLKI KICEAVSLKP TAWSLRHAVG PRPQTFLLDP YIALNVDDSR IGQTATKQKT NSPAWHDEFV TDVCNGRKIE LAVFHDAPIG YDDFVANCTI QFEELLQNGS RHFEDWIDLE PEGKVYVIID LSGSSGEAPK DNEERVFRER MRPRKRQGAV RRRVHQVNGH KFMATYLRQP TYCSHCRDFI WGVIGKQGYQ CQVCTCVVHK RCHELIITKC AGLKKQETPD EVGSQRFSVN MPHKFGIHNY KVPTFCDHCG SLLWGLLRQG LQCKVCKMNV HRRCETNVAP NCGVDARGIA KVLADLGVTP DKITNSGQRR KKLAAGAESP QPASGNSPSE DDRSKSAPTS PCDQELKELE NNIRKALSFD NRGEEHRASS ATDGQLASPG ENGEVRPGQA KRLGLDEFNF IKVLGKGSFG KVMLAELKGK DEVYAVKVLK KDVILQDDDV DCTMTEKRIL ALARKHPYLT QLYCCFQTKD RLFFVMEYVN GGDLMFQIQR SRKFDEPRSR FYAAEVTSAL MFLHQHGVIY RDLKLDNILL DAEGHCKLAD FGMCKEGIMN GVTTTTFCGT PDYIAPEILQ ELEYGPSVDW WALGVLMYEM MAGQPPFEAD NEDDLFESIL HDDVLYPVWL SKEAVSILKA FMTKNPHKRL GCVAAQNGED AIKQHPFFKE IDWVLLEQKK IKPPFKPRIK TKRDVNNFDQ DFTREEPILT LVDEAIIKQI NQEEFKGFSY FGEDLMP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays essential roles in the regulation of multiple cellular processes linked to cytoskeletal proteins, such as cell adhesion, motility, migration and cell cycle, functions in neuron growth and ion channel regulation, and is involved in immune response, cancer cell invasion and regulation of apoptosis. Mediates cell adhesion to the extracellular matrix via integrin-dependent signaling, by mediating angiotensin-2-induced activation of integrin beta-1 (ITGB1) in cardiac fibroblasts. Phosphorylates MARCKS, which phosphorylates and activates PTK2/FAK, leading to the spread of cardiomyocytes. Involved in the control of the directional transport of ITGB1 in mesenchymal cells by phosphorylating vimentin (VIM), an intermediate filament (IF) protein. In epithelial cells, associates with and phosphorylates keratin-8 (KRT8), which induces targeting of desmoplakin at desmosomes and regulates cell-cell contact. Phosphorylates IQGAP1, which binds to CDC42, mediating epithelial cell-cell detachment prior to migration. During cytokinesis, forms a complex with YWHAB, which is crucial for daughter cell separation, and facilitates abscission by a mechanism which may implicate the regulation of RHOA. In cardiac myocytes, regulates myofilament function and excitation coupling at the Z-lines, where it is indirectly associated with F-actin via interaction with COPB1. During endothelin-induced cardiomyocyte hypertrophy, mediates activation of PTK2/FAK, which is critical for cardiomyocyte survival and regulation of sarcomere length. Plays a role in the pathogenesis of dilated cardiomyopathy via persistent phosphorylation of troponin I (TNNI3). Involved in nerve growth factor (NFG)-induced neurite outgrowth and neuron morphological change independently of its kinase activity, by inhibition of RHOA pathway, activation of CDC42 and cytoskeletal rearrangement. May be involved in presynaptic facilitation by mediating phorbol ester-induced synaptic potentiation. Phosphorylates gamma-aminobutyric acid receptor subunit gamma-2 (GABRG2), which reduces the response of GABA receptors to ethanol and benzodiazepines and may mediate acute tolerance to the intoxicating effects of ethanol. Upon PMA treatment, phosphorylates the capsaicin- and heat-activated cation channel TRPV1, which is required for bradykinin-induced sensitization of the heat response in nociceptive neurons. Is able to form a complex with PDLIM5 and N-type calcium channel, and may enhance channel activities and potentiates fast synaptic transmission by phosphorylating the pore-forming alpha subunit CACNA1B (CaV2.2). Downstream of TLR4, plays an important role in the lipopolysaccharide (LPS)-induced immune response by phosphorylating and activating TICAM2/TRAM, which in turn activates the transcription factor IRF3 and subsequent cytokines production. In differentiating erythroid progenitors, is regulated by EPO and controls the protection against the TNFSF10/TRAIL-mediated apoptosis, via BCL2. May be involved in the regulation of the insulin-induced phosphorylation and activation of AKT1. Phosphorylates NLRP5/MATER and may thereby modulate AKT pathway activation in cumulus cells.
  • 基因功能参考文献：
    1. results demonstrate the mechanism for the maintenance of the undifferentiated state of embryonic stem cells via the inhibition of the FGF4-PKCzeta-MEK-ERK1/2 pathway by O-GlcNAcylation on PKCzeta. PMID: 29249667
    2. Data indicate that sirtuin 5 (SIRT5) is an important mitochondrial enzyme for protection against metabolic and ischemic stress following protein kinase C epsilon (PKCepsilon) activation in the brain. PMID: 27435822
    3. abnormal PKC-epsilon/PKC-delta ratio promotes the activation of Drp1 signalling, caspase cascades and GSK-3beta-dependent mPTP opening PMID: 28266127
    4. PKCepsilon deletion in the epidermis resulted in inhibition of UVR-induced Stat3 phosphorylation. PMID: 27102301
    5. Resistin-associated vascular smooth muscle cell dysfunction and intimal hyperplasia are related to PKCepsilon-dependent Nox activation and ROS generation PMID: 27573736
    6. PKCepsilon is a negative regulator of megakaryopoiesis PMID: 28783756
    7. study establishes that the pseudosubstrate of PKC-epsilon binds phosphatidylinositol 4-phosphate (PI4P), tethering it to the trans-Golgi network (TGN). This TGN-associated pool of PKC-epsilon is necessary for its concentration at the phagocytic cup and Fc [gamma] receptor (FcgammaR)-dependent membrane fusion PMID: 28539432
    8. that PKC-epsilon activation mediates 1) dysfunction of complexes I and III of the respiratory chain, 2) oxidant production, 3) morphological damage to the kidney, and 4) decreases in renal functions after ischemia PMID: 27760765
    9. Sstudy provides evidence that PKCepsilon-ALDH2 pathway positively regulates adipocyte differentiation by regulation of PPARgamma transcriptional activity. PMID: 27575855
    10. Data suggest that PKCe positively regulates beta-catenin expression and stabilization in a glycogen synthase kinase 3beta-independent manner; beta-catenin exhibits a perinuclear localization pattern in podocytes; however, beta-catenin is predominantly localized to nucleus in podocytes from PKCe knockout mice. (PKCe = protein kinase C epsilon) PMID: 28539358
    11. Results demonstrate that specific inhibition of PKCepsilon reduces ethanol consumption and prolongs ethanol intoxication, support the development of inhibitors of PKCepsilon catalytic activity as a strategy to reduce ethanol consumption, and they demonstrate that the AS- PKCepsilon mouse is a useful tool to study the role of PKCepsilon in behavior. PMID: 26947945
    12. PKCepsilon signaling in both the nucleus accumbens and central nucleus of the amygdala is a major contributor to binge alcohol drinking and to the genetic propensity to consume excessive amounts of alcohol. PMID: 25861702
    13. with strain there was significant PKCepsilon translocation to the Z-disc and co-localization with CapZbeta1 or alpha-actinin, which was quantified on confocal images. PMID: 26429793
    14. Protein kinase C epsilon phosphorylates the alpha4 nAChR subunit and regulates recovery from receptor desensitization. PMID: 26103136
    15. PKCepsilon negatively interferes with vessel progenitor differentiation via interaction with PAK-1 PMID: 25433270
    16. PKC-epsilon is the major isoform in the chiasmatic neurons. PMID: 24863469
    17. deficiency in kidney allografts attenuates ischemia/reperfusion induced apoptosis PMID: 25056349
    18. role in ethanol-induced steatosis PMID: 24483773
    19. PKCepsilon integrates with ERK1/2 to mediate both TPA and UVR-induced epidermal Stat3Ser727 phosphorylation PMID: 21480396
    20. HASF (GoPro49) is a stem cell paracrine factor that activates PKC epsilon mediated cytoprotection. PMID: 24269490
    21. In this mouse model of aging, decreases in Ciliary beat frequency are related to an increase in Protein Kinase C-epsilon activity. PMID: 24487393
    22. Vascular endothelial tight junctions and barrier function are disrupted by 15(S)-hydroxyeicosatetraenoic acid partly via protein kinase C epsilon-mediated zona occludens-1 phosphorylation at threonine 770/772. PMID: 24338688
    23. study is the first to report multiple nPKCepsilon-interacting proteins and their signaling pathways in HPC mouse brain, suggesting that nPKCepsilon signaling molecules is responsible for HPC-induced neuroprotection against cerebral ischemic injuries of mice PMID: 23665338
    24. selective activation of mitochondrial PKCepsilon preserves mitochondrial function in vitro and improves outcome in vivo after middle cerebral artery occlusion and 24 hr of reperfusion PMID: 23426889
    25. This study identifies PKCzeta as a novel key upstream regulator of BA-regulated SHP function, revealing the role of Thr-55 phosphorylation in epigenomic regulation of liver metabolism. PMID: 23824184
    26. PKCepsilon is widespread in the spinal nociceptive system and it appears to be specifically involved in mediating the synergistic interaction between delta opioid receptors and alpha2A adrenergic receptors PMID: 23946412
    27. seemingly strong expression of PKC in heart and ganglia shown in this study suggests a important role of this isoform in the vascular and nervous systems during mouse development PMID: 23639204
    28. PKC-epsilon can affect insulin uptake in mouse embryonic fibroblasts through promotion of receptor-mediated endocytosis, and that this may be mediated by regulation of CEACAM1 expression. PMID: 23469261
    29. Phosphorylation of serine 779 in fibroblast growth factor receptor 1 and 2 by protein kinase C(epsilon) regulates Ras/mitogen-activated protein kinase signaling and neuronal differentiation. PMID: 23564461
    30. PKC-epsilon is critical for cardiac cell early gene expression, acting as a regulator that has to be fine tuned in precursor cardiac cells. PMID: 22936275
    31. Data indicate that CGI-58 knockdown leads to increased diacylglycerol (DAG) acumulation in the lipid droplet/ER, preventing DAG accumulation and PKCepsilon translocation to the plasma membrane. PMID: 23302688
    32. Deletion of protein kinase Cepsilon in mice has limited effects on liver metabolite levels but alters fasting ketogenesis and gluconeogenesis. PMID: 22814763
    33. results reveal a greater than additive role for PKCtheta; and PKCepsilon in supporting platelet activation under shear conditions and demonstrate that, in combination, the two novel PKCs support platelet activation PMID: 22812584
    34. data suggest that in mice myometrium, phosphorylation of h1 calponin induced by the PKC-epsilon might facilitate the contraction of uterine in labor and regulate pregnant myometrial contractility. PMID: 22551221
    35. nPKCepsilon may play an important role in the development of cerebral hypoxic preconditioning. PMID: 21166186
    36. These data identify PKCepsilon as a key regulator of cilia slowing in response to combined smoke and alcohol-induced lung injury. PMID: 22677421
    37. re-establishes the participation of macrophages as a relay in the action of fatty acids on muscle cells, and further identifies PKCtheta; and PKCepsilon as key elements in the inflammatory and insulin resistance responses of muscle cells to macrophage products PMID: 22046423
    38. Non-small cell lung carcinoma cell motility, rac activation and metastatic dissemination are mediated by protein kinase C epsilon PMID: 22384062
    39. PKCepsilon-mediated phosphorylation at S142 increased NaV1.8 currents, lowered the threshold voltage for activation, & produced a depolarizing shift in inactivation in wild-type - but not in PKCepsilon-null - sensory neurons. PMID: 22426212
    40. PKCzeta is essential for Gq-dependent ERK5 activation in cardiomyocytes and cardiac fibroblasts and indicate a key cardiac physiological role for the Galpha(q)/PKCzeta/ERK5 signaling axis. PMID: 22232556
    41. Data show that in Prkce(-/-) mice, conditioned place preference and self-administer behaviors are associated with reduced levels of alpha(6) and beta(3) nicotinic receptor subunit mRNA in the ventral midbrain and striatum. PMID: 21911393
    42. Enhanced PKCepsilon mediated phosphorylation of connexin43 at serine 368 by a carboxyl-terminal mimetic peptide is dependent on injury. PMID: 21532342
    43. Prkce deletion ameliorates diet-induced glucose intolerance. PMID: 21347625
    44. partial blockade of PKC potentiates aggregation and dense granule secretion by ADP in association with increased Ca(2+) PMID: 21489985
    45. these results establish a previously uncharacterized role forPKC-epsilon, PKC-eta, PKC-theta in T cell polarity. PMID: 21602810
    46. Data suggest that PKCepsilon may be potential target for prevention and/or treatment of PCa. PMID: 21406403
    47. Using yeast cells co-expressing the human wild-type p53 and a single mammalian PKCalpha, delta, epsilon or zeta, results showed a differential regulation of p53-mediated apoptosis by these PKC isoforms. PMID: 21338602
    48. PKCepsilon overexpression leads to the pre-neoplastic lesions int the prostate, therefore suggesting a potential link between PKCepsilon and prostate caner development. PMID: 21224724
    49. Expression of the PKC-epsilon in coronary vessels is under control of the g-protein Galpha11. PMID: 21190563
    50. The study shows a correlation between fatty acids, protein kinase C epsilon phosphorylation and downregulation of insulin receptor. PMID: 21236337

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, cytoskeleton. Cell membrane. Cytoplasm, perinuclear region. Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily
  • 数据库链接：

    KEGG: mmu:18754

    STRING: 10090.ENSMUSP00000094873

    UniGene: Mm.24614