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  4. Recombinant Mouse Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 (Pomgnt1), partial

Recombinant Mouse Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1 (Pomgnt1), partial

  • 中文名称：
    小鼠Pomgnt1重组蛋白
  • 货号：
    CSB-YP821008MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Pomgnt1重组蛋白
  • 货号：
    CSB-EP821008MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Pomgnt1重组蛋白
  • 货号：
    CSB-EP821008MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Pomgnt1重组蛋白
  • 货号：
    CSB-BP821008MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Pomgnt1重组蛋白
  • 货号：
    CSB-MP821008MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pomgnt1
  • Uniprot No.：
  • 别名：
    Pomgnt1; Protein O-linked-mannose beta-1,2-N-acetylglucosaminyltransferase 1; POMGnT1; EC 2.4.1.-
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.
  • 基因功能参考文献：
    1. Findings suggest that PomGnT1 promotes GBM progression via activation of beta-catenin and may serve as a prognostic factor for glioma patient survival as well as a novel molecular target for anticancer therapy in malignant glioma. PMID: 25085363
    2. POMGnT1 gene knockout cause basement membrane disruptions in a mouse model of dystroglycanopathy. PMID: 23454088
    3. Results describe the effect of altered glycosylation of alpha-dystroglycan caused by inactivation of POMGnT1 in the retina. PMID: 21447391
    4. classical beta1,2-elongation and beta1,6-GlcNAc branching of O-mannose glycan structures are dependent upon the POMGnT1 enzyme and O-mannosylation is not limited solely to alpha-DG in the brain PMID: 21460210
    5. POMGnT1 knockout mice were viable but with reduced fertility and variable lifespan PMID: 20816176
    6. The present study provides the first genetic animal model to further dissect the roles of POMGnT1 in MEB disease. PMID: 16458488
    7. From these findings, we propose that fukutin forms a complex with POMGnT1 and may modulate its enzymatic activity. PMID: 17034757
    8. POMGnT1(-/-) muscle showed minimal pathological changes with very low-serum creatine kinase levels, and had normally formed muscle basal lamina, but showed reduced muscle mass, reduced numbers of muscle fibers, and impaired muscle regeneration. PMID: 19114101

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  • 亚细胞定位：
    Golgi apparatus membrane; Single-pass type II membrane protein.
  • 蛋白家族：
    Glycosyltransferase 13 family
  • 组织特异性：
    Expressed at basal body and daughter centriole of photoreceptor cells (at protein level).
  • 数据库链接：

    KEGG: mmu:68273

    STRING: 10090.ENSMUSP00000102107

    UniGene: Mm.196045